Subfamily distant hybridization method for Xenocypris davidi Bleeker and Erythroculter ilishaeformis Bleeker

A technology of red-billed red catfish and yellow-tailed catfish, which is applied in fish farming, application, climate change adaptation, etc., can solve problems such as difficulties in distant hybridization, and achieve the effect of improving fertilization rate and hatching rate, and uniform traits.

Active Publication Date: 2013-01-30
HUNAN NORMAL UNIVERSITY
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  • Abstract
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Problems solved by technology

There are great differences in biological characteristics such as classification and body shape of these two fishes, so there are great difficulties in the distant hybridization between the two fishes

Method used

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  • Subfamily distant hybridization method for Xenocypris davidi Bleeker and Erythroculter ilishaeformis Bleeker
  • Subfamily distant hybridization method for Xenocypris davidi Bleeker and Erythroculter ilishaeformis Bleeker
  • Subfamily distant hybridization method for Xenocypris davidi Bleeker and Erythroculter ilishaeformis Bleeker

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Embodiment

[0017] A method for distant hybridization between the yellow-tailed pomfret of the present invention and the red pomfret subfamily, specifically comprising the following steps:

[0018] 1. Artificial induction: the female yellow-tailed pomfret is used as the female parent fish, and the male red pomfret is used as the male parent fish. In late June every year, female yellow-tailed pomfrets over two years old, with obvious sexual maturity characteristics and good physical signs are selected. The pomfret and the male red pomfret were induced to give birth. First inject the mixed oxytocic agent of lutein-releasing hormone analogue (LRH-A) and chorionic gonadotropin (HCG) to the mother brood fish to induce labor. The injection dosage of LRH-A is 20μg / kg, and the injection dosage of HCG is 1500IU / kg, using 45° pleural injection at the base of the pectoral fin depression without scales; after 4h to 5h, inject the male parent fish, the injection dose of the male parent fish is halved...

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Abstract

The invention discloses a subfamily distant hybridization method for Xenocypris davidi Bleeker and Erythroculter ilishaeformis Bleeker. The method comprises the following steps: by using Xenocypris davidi Bleeker as a female parent fish and Erythroculter ilishaeformis Bleeker as a male parent fish, firstly carrying out artificial induced spawning on the female parent fish and the male parent fish, carrying out artificial dry-method insemination on the female parent fish and the male parent fish which have good induced spawning effects, incubating with flowing water in a culture dish after insemination, farming after fries are incubated, and obtaining filial generations of Xenocypris davidi bleeker and Erythroculter ilishaeformis Bleeker by detection after breeding. The subfamily distant hybridization method provided by the invention has the advantages of simple steps, convenient operation, high fertility rate and hatching rate, good characters for later generations and the like.

Description

technical field [0001] The invention relates to a method for distant hybridization among fish subfamilies, in particular to a method for distant hybridization between yellow-tailed pomfret and red-billed pomfret subfamily. Background technique [0002] Distant hybridization of fish is the hybridization between two different species of fish, which can be the hybridization of different species of fish of the same genus, or the individual fishes of different genera, different subfamilies, different families or even different orders hybridization between. Hybridization can integrate the advantages of both parents, so that the hybrid offspring will show heterosis in terms of appearance, growth speed, survival rate and disease resistance. It is an important method for fish genetic breeding and trait improvement. Yellow-tailed pomfret ( Xenocypris davidi Bleeker ) belongs to the diploid fish (2n=48) in Cyprinidae and Pomfret subfamily, and has the advantages of good meat quality ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A01K67/027
CPCY02A40/81
Inventor 刘少军肖军陶敏张纯罗凯坤刘筠谢丽华
Owner HUNAN NORMAL UNIVERSITY
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