Efficient breeding method for safety thermo-sensitive genic sterile line and two-line hybrid rice

Abstract

The invention belongs to the technical field of hybrid rice and discloses an efficient breeding method for a safety thermo-sensitive genic sterile line and two-line hybrid rice. The genetic analysis for low-temperature sterile characteristics of lifelong sterile strain ZHS of thermo-sensitive genic sterile line finds that the critical sterile temperature of the thermo-sensitive genic sterile line is regulated under the combined action of fertile conversion regulating gene (A) and repressor (B); the critical sterile temperature of the AAbbss sterile strain is ultralow and the AAbbss sterile strain is lifelong sterile; the critical sterile temperature of the aaBBss sterile strain is higher and the aaBBss sterile strain is normally transformed under the low temperature in local place and Sanya City of Hainan Province; the critical sterile temperature of the AABBss sterile strain meets the requirement of safety thermo-sensitive genic sterile line. According to the invention, the safety thermo-sensitive genic sterile line is bred in the manner of continuous selfing of the sterile strain with the genotype of aaBBss in F2 group and paired crossing and continuous backcross with the thermo-sensitive genic sterile line with the genotype of AABBss. According to the invention, the investment is less, the breeding efficiency is high, the seeding period is short and the mistaken elimination of breeding materials is avoided.

Description

technical field [0001] The invention belongs to the technical field of hybrid rice, and in particular relates to a high-efficiency breeding method for a safe thermosensitive male sterile line and a two-line hybrid rice. Background technique [0002] Compared with three-line hybrid rice, two-line hybrid rice has the advantages of dual-purpose in one line, free combination, and easy utilization of heterosis between subspecies, so it has been widely used in production. At present, most of the two-line male sterile lines used on a large scale in production are temperature-sensitive male sterile lines whose sterile gene is tms5. There is a great risk of reproduction and seed production in utilization. The critical sterile temperature is a key indicator of whether this type of temperature-sensitive GMS line has production and utilization value. Breeding a temperature-sensitive GMS line with an appropriate critical sterility temperature, that is, a safe temperature-sensitive GMS l...

AI Technical Summary

Problems solved by technology

[0003] To sum up, the problems existing in the existing breeding methods are: there are errors in the theory on which the breeding methods are based; the investment is large and the cost is high; the breeding efficiency of excellent sterile lines is low; it is easy to cause the mistaken elimination of excellent breeding materials ; CMS breeding progress is slow and the breeding cycle is long

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