78 results about "Hormone analog" patented technology

The invention relates to an intermedin analogue prepared by bonding a ring core sequence with biotin or cell-penetrating peptides and a preparation method and application of the intermedin analogue. The intermedin analogue is prepared by bonding a functional unit with the biotin or a load unit; the functional unit is a seven-peptide structure formed by connecting the ring core sequence c (Asp-Dab-D-Phe-Arg-Trp-Lys) with a connection unit Arg, the structure is Arg-c (Asp-Dab-D-Phe-Arg-Trp-Lys); the load unit is the 48th-57th peptide segments Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg or the 55th-57th peptide segments Arg-Arg-Arg in the 47th-60th segments of HIV (human immunodeficiency virus)-1TAT protein. The intermedin analogue disclosed by the invention can penetrate through mucosa or skin to be absorbed, and can be applied to treatment of diseases such as male and female sexual dysfunction, obesity, pigmentation deficiency and the like.

The present invention relates to a composite microsphere system comprising poly(D,L-lactide-co-glycolide) (PLGA), poly(acryloyl hydroxyethyl starch) (AcHES), and a pharmaceutically effective amount of a biologically active compound. The active compound may be, for example, an insulin, an interferon, a luteinizing hormone-releasing hormone (LHRH) analog, a somatostatin and/or derivatives thereof, a calicitonin, a parathyroid hormone (PTH), a bone morphogenic protein (BMP), an erythropoietin (EPO), an epidermal growth factor (EGF) or a growth hormone. This invention also relates to methods of using the composite microspheres, and methods of preparing same.

A method for inducing melanogenesis in a human subject having a melanocortin 1 receptor (MC1R) variant allele associated with loss of or diminished receptor function comprises administering to said subject an amount of an α-melanocyte stimulating hormone (α-MSH) analogue effective to induce melanogenesis by the melanocytes in the skin or other epidermal tissue of the subject.

The invention discloses an artificial propagation method for Taiwan mud fishes and belongs to the propagation technologies for fresh water mud fishes. The method includes the following steps that (1), parent mud fish fries are selected; (2), artificial spawning induction is performed; (3), artificial fertilization is performed; (4), hatching is performed. According to the technology, the method that human chorionic gonadotropin (HCG), hypophyses or luteotropin releasing hormone d-ala analogs and other spawning induction medicine are injected to the backs of the parent mud fishes is adopted, so that the spawning induction rate of the parent mud fishes is increased over 91.0%; according to the technology, the fertilization rate of the parent mud fishes is increased over 96.6% through artificial fertilization, and the hatching rate of the parent mud fishes is increased over 98.0% through still water hatching; the survival rate of the parent mud fishes after artificial propagation reaches 90.9%. Propagation is performed by the utilization of the method, so that the survival rate, the spawning induction rate, the fertilization rate and the hatching rate of the Taiwan mud fishes are much higher than the corresponding rates through natural propagation and traditional propagation method for the mud fishes, industrialized production of the Taiwan mud fishes can be achieved, the fries are provided for large-scale farming, and high economical benefits are obtained.

The invention relates to an artificial induced spawning method of Glyptosternum maculatum Regan originally produced in Yarlung Zangbo river in Tibet. The invention discloses an artificial induced spawning method of the Glyptosternum maculatum Regan. By adopting the exogenous induced-spawning medicine, the gonads of the naturally matured parent Glyptosternum maculatum Regans are promoted to further develop and breed by using the artificial induced spawning method so as to obtain fertilized eggs. The method comprises the following steps: (1) the mature parent Glyptosternum maculatum Regans selected and are matched according to the number ratio of 2-3:1 of the male parent Glyptosternum maculatum Regan and the female parent Glyptosternum maculatum Regan; (2) the induced-spawning medicine composition is prepared, and the dosage of the induced-spawning medicine composition for the female parent Glyptosternum maculatum Regan is 2mg domperidone maleate/kg (weight) + 10-15mg luteotropin releasing hormone d-ala analog/kg (weight) + 3-5mg carp pituitary/kg (weight); the dosage of the induced-spawning medicine composition for the male parent Glyptosternum maculatum Regan is one seconds of that of the induced-spawning medicine composition for the female parent Glyptosternum maculatum Regan; (3) the induced-spawning medicine is prepared to be pasty according to the formula; and (4) an administration method of injection by two needles is provided. The effect time of the prepared induced-spawning medicine composition for the male parent Glyptosternum maculatum Regan and the female parent Glyptosternum maculatum Regan is 10-12h; and the method can achieve the induced spawning ratio of 85 percent.

The invention relates to a method for inducing gonad maturity and ovulation of a verasper variegates parent fish by implanting a slow-release hormone, which comprises two aspects of cultivating an optimally selected parent fish and adjusting temperature and light to promote the gonad development of the parent fish, and implanting the slow-release hormone for promoting a metrocyte of the parent fish to reach final maturity and ovulation. In the invention, the slow-release hormone is a salmon gonadotropin-releasing hormone analog (sGnRHa) and is coated with a safe and high-efficiency polymer slow-release transitive system (95 percent of cholesterol and 5 percent of cellulose); a female parent fish with gonad in fourth stage is selected by using a living body sampling method or a light table method; a special implanting gun is used for implanting in back muscles, and the effect time of the slow-release hormone lasts for 14-18 days. The method can enable the metrocyte of the verasper variegates parent fish to achieve final maturity and ovulation, has low duress on parent fish reproduction, high rate of parent fish survival and gonad maturity, high fertility rate being 72 percent and high hatching rate being 56.2 percent, and can acquire a bulk of high-quality oosperms. The invention is a set of complete control technique method, can stably regulate and control the development, the maturity and the ovulation of the gonad of the verasper variegates parent fish, and acquire high-quality oosperms.

The invention provides a method for breeding tridentiger trigonocephalus by artificial induced spawning and insemination, which relates to the artificial propagation of gobies. The method required tobe provided for artificially breeding the tridentiger trigonocephalus comprises the steps of ripe fish screening, maturity acceleration, insemination, incubation and larval fish culture, wherein the maturity acceleration of ripe fishes uses luteotropin to release hormone analogues, domperidone maleate and chorionic gonadotropin, and is characterized in that the three hormones are used twice for the maturity acceleration; in the maturity acceleration, the water temperature of culture is between 18 and 21 DEG C, the salinity is between 2 and 6, the dissolved oxygen is between 3.5 and 6.5mg/L, and the pH is between 7.5 and 9.0; and after the second time of the maturity acceleration, in 8 to 10 hours, mature ova and mature sperms are selected for artificial insemination which comprises the following steps that: the activated sperms are poured on the ova for sufficient contact so as to obtain fertilized ova; the fertilized ova are placed in a hatchery pond for incubation; and the hatched larval fish is placed in a culture pond for incubation, and chlorella and yolk powder of eggs are placed in water from the third day after the larval fish is hatched to feed food for the larval fish. The method is used for the artificial breeding of the tridentiger trigonocephalus.

The invention discloses a method for batch breeding of loach fry, which notably improves catalytic production rate of the loach. The method comprises the steps of: a, selecting loach parent for breeding to perform prenatal strengthening cultivation; b, building a third-level filtering reservoir device for spawning and hatching; c, selecting composite of the maleic acid domperidone and the trout luteinizing hormone releasing hormone analogue as a catalyst to catalyze male and female loaches, wherein per kilogram of female loaches need 10-15mg of the catalyst; per kilogram of male loaches need 5-7.5mg of the catalyst; enabling the female loaches and the male loaches to spawn and inseminate in a net cage in the jar according to the ratio of the female loaches to the male loaches being 1:2; taking out the loaches, enabling the spawns to continuously hatch; producing loach fry in a large scale; d, feeding the initial feed, putting the loaches to the pond; according to organ development characteristic of the loach fry, controlling diet conversion time point, electing palatable bait, scientifically feeding the bait, and strengthening water quality and disease control and prevention.

The invention discloses a loach fry cultivation method. In April in spring, when a natural water body is higher than 18 DEG C in temperature, mature loaches which are regular in body form, strong in physique, much in mucus, healthy and scot-free are used as parents; LRH-A2 and DOM are used for hastening parturition; incubating is carried out at the water temperature which is 25 DEG C after oviposition, and loach fry cultivation is carried out after yolk sacs of most fries are basically disappeared after loach fry incubation; the loach fries are transferred to a pond to conduct cultivation according to a conventional summer fingerling cultivation technology after cultivation is carried out for seven days. According to the loach fry cultivation method, mixed feeding is carried out with yolk liquid and juvenile prawn mixed feed, the nutritional requirement of the mouth-opening periods of the loach fries is met, the loach fries rapidly grow, the complex process of rotifer cultivation is eliminated, the whole cultivation process is easy to operate, the labor amount is greatly reduced, meanwhile, the loach fry pond entering cultivation specification is increased, and the fry cultivation survival rate is improved.

The invention relates to the field of aquaculture, in particular to a manual induced spawning method for platichthys stellatus parental fishes. Trout gonadotrophic hormone releasing hormone analogues (S-GnRHa) and domperidone (DOM) are used for performing induced spawning on the platichthys stellatus parental fishes, and female fishes older than 2.5 years and male fishes older than 2 years are selected for being put into a parental fish breeding pond for ripening acceleration. The sexual maturity is controlled in an intensifying mode through water temperature, illumination stimulation and baits. The S-GnRHa and DOM are used for performing induced spawning on the platichthys stellats parental fishes, medication injection is performed on the female and male parental fishes two times according to weights, so that the effect of performing manual induced spawning on the platichthys stellatus parental fishes is achieved and artificial insemination is performed. According to the method, the platichthys stellatus parental fishes are matured synchronously, spawning can be performed within a preset period in a concentrated mode, the spawning quantity is large, the spawning period is shortened by 10-15 days, the insemination rate reaches 80%, the hatching rate reaches 70%, manual induced spawning is performed on the parental fishes, the success rate of performing induced spawning on the male fishes reaches 98%, and the success rate of performing induced spawning on the female fishes reaches 95%.

A method for preparing sturgeon caviar from eggs collected from live sturgeon is characterized in that the method comprises following steps: selecting sturgeon whose ovary develops to the end of IV stage, injecting luteinizing hormone-releasing hormone analogue, hocusing the sturgeon with water containing clove oil 80 to 120 ppm when the ovary is completely free, cutting the belly to collect eggs, sterilizing eggs at 0 to 4 DEG C, adding non-iodized salt to prickle the eggs, draining with a screen, sterilizing with UV ray, suturing the sturgeon, disinfecting with medical hydrogen peroxide solution, injecting gentamicin with 2,000 to 20,000 international units, and temporarily culturing in clean water tank. The method is simple, convenient and needs no complex equipment. The caviar can be made from sturgeons fished naturally or cultured sturgeons without killing the sturgeons. The survival sturgeons are reintroduced into the original water area, and the survive rate is ensured to above 80%, thus protecting resources, achieving sustainable use, improving the utilization efficiency of sturgeons, and maintaining the ecological balance.

The invention relates to a method for inducing hormone soaked loaches. The method comprises the following steps: (1) selecting a mature loach parent in water at the temperature of between 21 and 29 DEG C; and (2) taking a mixture of domperidone maleate, luteotropin releasing hormone d-ala analog and chorionic gonadotropin as oxytocic hormones, and soaking the loach parent with a soaking solution which is prepared by mixing the oxytocic hormone, pentoxifylline and edible oil in normal saline, wherein the dosage of the domperidone maleate is between 100 and 300mg/kg, the dosage of the luteotropin releasing hormone d-ala analog is between 200 and 600mu g/kg, the dosage of the chorionic gonadotropin is between 15,000 and 30,000IU/kg, and the dosage of the normal saline is between 0.2 and 0.3kg/kg; and when the loach parent is soaked, the temperature of the soaking solution is controlled to be between 21 and 29 DEG C, and the soaking time is controlled to be between 0.5 and 8 hours. The aim of inducing the loach parent can be realized through soaking instead of injection.

The invention relates to a complete artificial propagation method for acrossocheilus fasciatus. The method comprises: acquiring wild acrossocheilus fasciatus juvenile fishes for acclimation, culturing until sex matured, using exogenous parturifacient chorionic gonadotropin (HCG), lutropin releasing hormone analog (LHRH-A3) and domperidone (DOM), through a one-time injection method, to promote sexual gland of parent fishes of acrossocheilus fasciatus which is mature naturally to develop further, through dry insemination to obtain oosperms, and through a special hatching equipment, performing flowing hatching until hatching. Rate of inducing spawning of parent fishes reaches up to 85%, and fertility rate reaches above 85%, and hatchability is above 70%. The method solves problems of large-scale production of acrossocheilus fasciatus fries.

The invention discloses an artificial spawning induction method for rhinogobio ventralis. According to the method, the male and female proportion of mature parent fishes is 1:1.5; spawning induction medicine is lutropin releasing hormone analogies, and the dosage of the medicine is 6 micrograms per kilogram of fish weight; according to an artificial fertilization method, female fishes awaiting spawning are caught, moisture of fish bodies are wiped to be dry, ova are extruded into a dry container, then male fishes awaiting spawning are caught, genital openings are wiped to be dry, seminal fluid is sucked up through an injector, placed in a sperm dilution and then mixed with the ova, activation is performed by clear water, standing is performed for two minutes, and then fertilized ova are poured into an incubator for incubation; the flow speed of water bodies in the incubator before stripping of the fertilized ova is 0.4 m/s, the flow speed after stripping is 0.2 m/s, after stripping, the fertilized ova grow in the incubator until fish fry swim bladders are inflated, and then fish fry cultivation can be performed. The spawning induction rate is larger than 65%, the fertilization rate is larger than 85%, the incubation rate is larger than 80%, a large number of fish fries can be provided for batch production of the fish fries of the specification of rhinogobio ventralis, and therefore proliferation liberation of the rhinogobio ventralis is achieved.

A method for controlling the maturation of sex gland of verasper and kareius features that a slow-releasing hormone for cold-water fish, which contains water, gelatin, gonad stimulating hormone or ganodotropic releasing hormone or luteinizing hormone, etc. is embedded in the artificially cultured parents of verasper and kareius.

The invention discloses a breeding method for distant hybridization between erythroculter ilishaeformis and ancherythropterus nigrocauda. The method includes the following steps: 1) selecting selectively-bred erythroculter ilishaeformis and selectively-bred ancherythropterus nigrocauda as parents; 2) conducting parents strengthening breeding including erythroculter ilishaeformis strengthening breeding and ancherythropterus nigrocauda strengthening breeding; 3) conducting artificial spawning induction: respectively injecting spawning induction medicine hypophysis cerebri or dopamine antagonist DOM, lutein releasing hormone analogue and chorionic gonadotropin which are used singly or in mixing mode on the two parents; 4) conducting artificial semen and spawn collection and insemination including dry method insemination and semi-dry method insemination; and 5) conducting artificial incubation. Hybridized culter 'vanguard 1' obtained by distant hybridization has the advantages of good body type and large size of the erythroculter ilishaeformis, also has the advantages of low breeding cost, mild temper, easiness in catching and easiness in live marketing of the ancherythropterus nigrocauda and further has excellent hybridization performances like high growing speed.

The extraction method of insect molting hormone analog in bulge relates to extraction of botanical insect hormone analog. The extraction method of insect molting hormone analog in bulge is one combining alcohol extraction, water precipitation and ultrasonic extraction. Through ultrasonic vibration and repeated extraction of the residue, the present invention has high extracting rate, high extract purity, simple extraction process and no environmental pollution.

The invention relates to the fields of molecular biology and biological technology, and discloses Eriocheir sinensis GnRH (gonadotropin-releasing hormone) analogues with respective amino acid sequence of QSYHFSHGWKP and QAYHFSHGWKP . Compared the the amino acid sequences of the two subtypes of Eriocheir sinensis GnRH analogues with those of the GnRH of other species, the results show that the former and the latter have similar amino acid conservative sites; the the sequence structures of the Eriocheir sinensis GnRH analogues are similar to that of invertebrate GnRH; and the Eriocheir sinensis GnRH analogues have similar functions as vertebrate GnRH, can stimulate breakdown of oocyte germinal vesicle and have distinct stimulative effect on crab oocyte maturation and important application value for artificial ripening of the Eriocheir sinensis.

The invention relates to an injection oxytocin for loach. The oxytocin is mixed from three hormones of DOM, LRHA and chorionic gonadotropin, wherein an amount of the DOM is 45-55mg/kg; an amount of the LRHA is 90-110[mu]g/kg, and an amount of the chorionic gonadotropin is 7000-8000IU/kg. According to the invention, the three hormones are reasonably proportioned to realize an objective of low usage amount and high induction efficiency.