Method for polymerizing plurality of one-way cross-incompatible genes of corn

Abstract

The invention discloses a method for polymerizing a plurality of one-way cross-incompatible genes of corn. The risk that a single Ga-S gene is subjected to outcrossing seed set when being used as an isolation tool is solved aiming at solving the difficulty in polymerizing a plurality of different one-way cross-incompatible genes. The method is characterized in that the affinity of F1 of a Ga-S gene is utilized, and Ga1-S and Ga2-S are polymerized by pollinating the F1, selfing offspring and being assisted by closely linked molecular marker selecting. In the corn seed production process, the one-way cross-incompatible gene is used for reproduction isolation, the outcrossing risk is reduced, reproduction isolation of different types of corns by using the one-way cross-incompatible gene in production is further promoted, and a large amount of manpower, material resources and financial resources are saved.

Description

technical field [0001] The invention belongs to the field of creation of new germplasm of crops. It specifically relates to a method for aggregating multiple maize unidirectional hybridization incompatibility genes. Background technique [0002] Maize is a typical cross-pollination crop. Normally, it can accept its own pollen and other corn's pollen to produce fruit. However, there are some types of corn in nature that break this rule: they only accept the pollen of the same type of corn, and do not accept the pollen of non-identical corn. At the same time, they can also pollinate most non-identical corn. . This phenomenon is called unidirectional outcross incompatibility in maize. Because it is related to the transmission of male and female gametes in maize, the gene controlling this phenomenon is called Gametophyte factor, that is, the unidirectional hybridization incompatibility Ga gene. [0003] The earliest Ga genes in nature only exist in popcorn, a very small numb...

AI Technical Summary

Problems solved by technology

Subsequent studies have shown that there are a few materials in common cultivated maize, which can penetrate the incompatibility of Ga1-S, Ga2-S and Tcb1-S respectively. The existence of this type of material limits the use of a single Ga gene in maize production. The application potential of different Ga-S genes in the same material can solve this problem

However, since Ga1-S and Ga2-S are incompatible with each other, that is, the hybridization between the two is not strong, how to aggregate the two sites of Ga1-S and Ga2-S into one is extremely difficult and unprecedented matter

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