Method for polymerizing plurality of one-way cross-incompatible genes of corn

A gene and affinity technology, which is applied in the field of new crop germplasm creation, can solve the problems of mutual hybridization, lack of fruit, and limit the application potential of a single Ga gene

Inactive Publication Date: 2021-06-04
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI +1
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Subsequent studies have shown that there are a few materials in common cultivated maize, which can penetrate the incompatibility of Ga1-S, Ga2-S and Tcb1-S respectively. The existence of this type of material limits the use of a single Ga gene in maize production. The application potential...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for polymerizing plurality of one-way cross-incompatible genes of corn
  • Method for polymerizing plurality of one-way cross-incompatible genes of corn
  • Method for polymerizing plurality of one-way cross-incompatible genes of corn

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0015] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0016] Maize inbred lines SDGa25 (genotype Ga1-SGa1-Sga2ga2) and Zheng 58 (genotype ga1ga1ga2ga2) were collected and preserved in our laboratory. Inbred line 511L (genotype ga1ga1Ga2-SGa2-S) is preserved by Maize GeneticsCooperation Stock Center, Co-op ID: 511L; Description: 511L Ga2-S(mexicana).

[0017] The present invention will be described in detail below with reference to the polymerization examples of Ga1-S and Ga2-S.

[0018] 1) In the winter of 2013, Hainan Ledongnan Breeding Base, with Zheng 58 (genotype ga1ga1ga2ga2) as the female parent, SDGa25 (genotype Ga1-SGa1-Sga2ga2), 511L (genotype ga1ga1Ga2-SGa2-S) respectively As the female parent, get the corresponding F1.

[0019] 2) In the summer of 2014, two F1s crosse...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for polymerizing a plurality of one-way cross-incompatible genes of corn. The risk that a single Ga-S gene is subjected to outcrossing seed set when being used as an isolation tool is solved aiming at solving the difficulty in polymerizing a plurality of different one-way cross-incompatible genes. The method is characterized in that the affinity of F1 of a Ga-S gene is utilized, and Ga1-S and Ga2-S are polymerized by pollinating the F1, selfing offspring and being assisted by closely linked molecular marker selecting. In the corn seed production process, the one-way cross-incompatible gene is used for reproduction isolation, the outcrossing risk is reduced, reproduction isolation of different types of corns by using the one-way cross-incompatible gene in production is further promoted, and a large amount of manpower, material resources and financial resources are saved.

Description

technical field [0001] The invention belongs to the field of creation of new germplasm of crops. It specifically relates to a method for aggregating multiple maize unidirectional hybridization incompatibility genes. Background technique [0002] Maize is a typical cross-pollination crop. Normally, it can accept its own pollen and other corn's pollen to produce fruit. However, there are some types of corn in nature that break this rule: they only accept the pollen of the same type of corn, and do not accept the pollen of non-identical corn. At the same time, they can also pollinate most non-identical corn. . This phenomenon is called unidirectional outcross incompatibility in maize. Because it is related to the transmission of male and female gametes in maize, the gene controlling this phenomenon is called Gametophyte factor, that is, the unidirectional hybridization incompatibility Ga gene. [0003] The earliest Ga genes in nature only exist in popcorn, a very small numb...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H1/02C12Q1/6895
CPCA01H1/02C12Q1/6895C12Q2600/13
Inventor 陈化榜陈智斌李凯张照贵
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap