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Cultivation method of gynogenesis megalobrama amblycephala

A technology of gynogenesis and breeding method, which is applied in the field of fish breeding to achieve the effect of shortening breeding time and facilitating genetic research

Active Publication Date: 2010-06-09
HUNAN NORMAL UNIVERSITY
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  • Claims
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Problems solved by technology

Although the heterologous sperm has been inactivated, part of the genetic material of the heterologous sperm can be integrated into the genetic material of the egg through the "heterosperm effect", which may have a positive effect on the offspring of gynogenesis

Method used

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Embodiment

[0017] 2 to 3 months before the breeding period, select female bream and male red bream individuals with obvious sexual maturity, good body shape, bright color, strong physique, and no disease or injury as broodstock for intensive breeding in special ponds. Feeding feed to promote the good development of broodstock gonads.

[0018] In the breeding season, when the water temperature is stable at 24°C to 25°C, select the above-mentioned broodstock with good signs and well-developed gonads for artificial induction, and inject a mixture of HCG and LRH-A. Inject female bream first, the dosage of HCG is 800-1000IU / kg, and the dosage of LRH-A is 20-30μg / kg, then inject male red bream 3-4 hours later, the dose is halved; after injection, press 1 : The quantity ratio of about 1.3 put the female and male fish into the same circular spawning pool, and hang a screen mesh on the water surface to detect the estrus and spawning of the broodstock; the injections are all injected into the abdo...

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Abstract

The invention discloses a cultivation method of a gynogenesis megalobrama amblycephala, which comprises the following steps of: firstly selecting and breeding a female megalobrama amblycephala and a male erythroculter ilishaeformis bleeker as parent fishes; then carrying out artificial induced spawning to obtain the sperms of the erythroculter ilishaeformis bleeker and the ripe ovums of the megalobrama amblycephala; mixing the erythroculter ilishaeformis bleeker sperms inactivated by ultraviolet rays with the ripe ovums of the megalobrama amblycephala; 3-4 minutes later, placing the ovums activated at 0-4 DEG C for cold shock treatment for 20-30 minutes; and after incubating embryos treated by cold shock at 23-24 DEG C, obtaining diploid gynogenesis megalobrama amblycephalas through a flow type cell DNA content determination method and a chromosome ploidy detection method of peripheral blood cell culturing. The cultivation method of the gynogenesis megalobrama amblycephala has the advantages of short cultivation time, fast growth speed, high incubation rate, excellent offspring property, and the like, and has great importance on aspects of fish inheritance breeding and biological evolution researches.

Description

technical field [0001] The invention relates to a method for cultivating fish, in particular to a method for gynogenesis of carps. Background technique [0002] Gynogenetic technology plays an important role in fish purification and breeding, research on fish sex determination and fish parthenoculture. Generally, artificial gynogenetic fish is cultivated by using heterologous sperm to activate the development of haploid eggs, and then the eggs undergo chromosome doubling to form diploid offspring that mainly rely on the genetic material of eggs to develop. Although the heterologous sperm has been inactivated, part of the genetic material of the heterologous sperm can be integrated into the genetic material of the egg through the "heterosperm effect", which may have a positive effect on the offspring of gynogenesis. However, since the vitality, density, and tolerance to radiation of sperm vary with the species, it is necessary to choose an effective stimulus source, a suitab...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 刘少军刘筠罗凯坤张纯陶敏赵如榕肖俊何伟国肖军
Owner HUNAN NORMAL UNIVERSITY
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