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Cultivation method of gynogenesis megalobrama amblycephala

A technology of gynogenesis and breeding methods, applied in the field of fish breeding, to achieve the effect of shortening breeding time and facilitating genetic research

Active Publication Date: 2011-07-27
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

Although the heterologous sperm has been inactivated, part of the genetic material of the heterologous sperm can be integrated into the genetic material of the egg through the "heterosperm effect", which may have a positive effect on the offspring of gynogenesis

Method used

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Embodiment

[0017] In the first 2 to 3 months of the breeding period, select individual females with obvious sexual maturity, good body shape, bright body color, strong physique, disease-free and harmless, as broodstock for intensive breeding in special ponds, which is reasonable Feeding feed to promote good development of broodstock gonads.

[0018] In the breeding season, when the water temperature stabilizes at 24°C to 25°C, the broodstock with good physical signs and well-developed gonads are selected for artificial induction and injected with a mixture of HCG and LRH-A. Inject the female bream first, the dosage of HCG is 800~1000IU / kg, the dosage of LRH-A is 20~30μg / kg, 3~4h later, then the male bream is injected, the dose is halved; after injection, press 1 :The number ratio is about 1.3. Put the female and male fish into the same round spawning pool, and hang a screen mesh on the water surface to detect the oestrus and spawning of the broodstock; the injection is performed in the abdo...

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Abstract

The invention discloses a cultivation method of a gynogenesis megalobrama amblycephala, which comprises the following steps of: firstly selecting and breeding a female megalobrama amblycephala and a male erythroculter ilishaeformis bleeker as parent fishes; then carrying out artificial induced spawning to obtain the sperms of the erythroculter ilishaeformis bleeker and the ripe ovums of the megalobrama amblycephala; mixing the erythroculter ilishaeformis bleeker sperms inactivated by ultraviolet rays with the ripe ovums of the megalobrama amblycephala; 3-4 minutes later, placing the ovums activated at 0-4 DEG C for cold shock treatment for 20-30 minutes; and after incubating embryos treated by cold shock at 23-24 DEG C, obtaining diploid gynogenesis megalobrama amblycephalas through a flow type cell DNA content determination method and a chromosome ploidy detection method of peripheral blood cell culturing. The cultivation method of the gynogenesis megalobrama amblycephala has the advantages of short cultivation time, fast growth speed, high incubation rate, excellent offspring property, and the like, and has great importance on aspects of fish inheritance breeding and biological evolution researches.

Description

Technical field [0001] The invention relates to a method for cultivating fish, in particular to a method for gynogenesis of cyprinids. Background technique [0002] Gynogenesis technology plays an important role in fish purification and breeding, exploring the sex determination of fish, and fish unisex culture. Generally, the cultivation of artificial gynogenesis fish uses heterologous sperm to activate the development of haploid eggs, and then the eggs undergo chromosome doubling to form diploid offspring that mainly rely on the genetic material of the eggs to develop. Although the heterologous sperm is inactivated, part of the genetic material of the heterologous sperm can be integrated into the genetic material of the egg through the "hetero-sperm effect", which may have a positive effect on gynogenesis offspring. However, since sperm motility, density, and tolerance to radiation are different from species to species, it is necessary to select an effective stimulation source,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 刘少军刘筠罗凯坤张纯陶敏赵如榕肖俊何伟国肖军
Owner HUNAN NORMAL UNIVERSITY
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