Cryopreservation solution for the semen of red catfish

A technology of cryopreservation liquid and red bream with cocked mouth, which is applied in the preservation, application, and animal husbandry of human or animal bodies

Inactive Publication Date: 2016-09-14
HUZHOU TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is still no unified conclusion on the composition of the cryopreservation solution so far, and it is generally believed that it should be screened one by one according to the characteristics of different fish sperm

Method used

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  • Cryopreservation solution for the semen of red catfish
  • Cryopreservation solution for the semen of red catfish
  • Cryopreservation solution for the semen of red catfish

Examples

Experimental program
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Effect test

Embodiment 1

[0014] 1. Preparation of cryopreservation solution for the semen of red catfish containing trehalose and glutamine

[0015] Preparation of diluent: Accurately weigh 7.896g of sodium chloride, 0.396g of potassium chloride, 0.195g of magnesium sulfate heptahydrate, 0.072g of disodium hydrogen phosphate dodecahydrate, 0.054g of potassium dihydrogen phosphate, 0.345g of sodium bicarbonate3, glucose Dissolve 0.99g in 1000mL distilled water, mix thoroughly, dispense into centrifuge tubes, and freeze in the refrigerator for later use.

[0016] Preparation of cryopreservative solution: add antifreeze ethylene glycol to the final concentration of 8% (volume percentage) in the prepared HBSS dilution, and the final concentrations of trehalose and glutamine are 34.23g / L and 2.9g / L respectively . That is to say, the composition of the cryopreservation solution for the semen of the red catfish per 1000mL is as follows:

[0017] Ethylene glycol 80mL

[0018] Trehalose 34.23g

[0019] Glu...

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Abstract

The invention relates to an erythroculter ilishaeformis semen cryopreservation solution. The erythroculter ilishaeformis semen cryopreservation solution takes a diluent as a matrix; each 1,000 mL cryopreservation solution contains 34.23g of trehalose, 2.9g of glutamine, and 8 percent (volume percentage) glycol. By adopting the cryopreservation solution disclosed by the invention, after erythroculter ilishaeformis semen is unfrozen, and the survival rate of semen is over 70 percent; compared with the fertilized eggs obtained by performing artificial insemination by fresh semen, the fertilized eggs obtained by performing the artificial insemination by frozen semens which are subjected to cryopreservation and anabiosis has the characteristic that the average hatching rates have no significant difference.

Description

technical field [0001] The invention relates to a cryopreservation solution for the semen of red catfish, which belongs to the technical field of aquaculture. Background technique [0002] Under the ultra-low temperature of liquid nitrogen (-196°C), the metabolism of the cells basically stops, and the sperm is in a state of suspended animation and can be stored for a long time. The cryopreservation of fish sperm is of great significance for fish breeding, resource protection and genetic improvement. Some fish species-specific sperm freezing protocols have been established. To freeze fish sperm, firstly dilute the semen in the cryopreservation solution, let it balance for a period of time, and then cool down and freeze it. Cryopreservation solution in addition to inorganic salts (such as NaCl, KCl, NaHCO 3 、KHCO 3 And sodium citrate, etc.) and penetrating antifreeze, often also add some non-permeable antifreeze. Impermeable antifreeze is a class of macromolecular substan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 采克俊曹访朱俊杰李景芬叶金云
Owner HUZHOU TEACHERS COLLEGE
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