Distant hybridization method for grass carp and erythroculter ilishaeformis subfamily

Abstract

The invention discloses a distant hybridization method for grass carp and an erythroculter ilishaeformis subfamily. The method comprises the following steps that the grass carp are adopted as female parent fishes, erythroculter ilishaeformis is adopdted as a male parent fish, the female parent fishes and the male parent fish are subjected to artificial hasten parturition in a breeding season, drymethod artificial insemination is carried out, and obtained germ cells are subjected to lotic hatching in a hatching trough; after fry are demoulded, all the fry hatch and point waists appear, farmingis carried out, detecting and screening are carried out, and diploid hybrid fishes and triploid hybrid fishes of the grass carp and the erythroculter ilishaeformis are obtained. According to the hybridization method, the advantages of herbivority, high growth speed and the slim figure of the female parent grass carp and the advantages of tender meat quality, high stress resisting and disease resisting ability, high growth speed and the like of the male parent erythroculter ilishaeformis are combined, the diploid hybrid fishes and the triploid hybrid fishes with obvious hybridization advantages are creatively cultivated, precious resources are provided for forming of subsequent novel grass carp strains, and important biological significance and economic value are achieved in the aspect offish genetic breeding and production application.

Description

technical field [0001] The invention relates to the technical field of distant hybridization of fish, in particular to a method for distant hybridization between grass carp and the subfamilies of the genus chinensis. Background technique [0002] Distant hybridization refers to the hybridization between species whose kinship is between species or above. It is an important method of fish genetics and breeding to quickly obtain significant trait improvement. It can integrate a whole set of exogenous genes, thereby changing the gene expression of hybrid offspring Regulation, so that the hybrid offspring may show heterosis in terms of meat quality, stress resistance, disease resistance and growth speed. [0003] The grass carp (Ctenopharyngodon idellus) adopted in the present invention belongs to Cyprinidae, Cyprinidae, the diploid fish (2n=48) in the subfamily of genus, generally reaches sexual maturity in 4 to 5 years, and grass carp is herbivorous Fish, which grow fast, are t...

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Problems solved by technology

However, due to its carnivorous nature, it may pose a threat to the survival and reproduction of other

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