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Seed production method by interspecific crossing of green abalone and Pacific abalone

A wrinkled plate abalone and hybrid seed production technology, which is applied in the fields of fish farming, application, climate change adaptation, etc., can solve the problems of green abalone self-propagation offspring loss, failure, and green abalone and wrinkled plate abalone have not yet been seen. Achieving high temperature resistance and remarkable growth rate

Active Publication Date: 2014-11-12
福建闽锐宝海洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of the introduction and cross breeding of green abalone, Wang Zichen (1985) introduced green abalone from California, USA, and carried out experiments on self-propagation of green abalone and hybridization with wrinkled pan abalone species. Difficult to adapt to survive in Dalian's climatic conditions, did not succeed
Xu Guoling et al. (Xu Guoling, Lao Zan, Yang Xiaoli, American green abalone domestication and artificial breeding, Tropical Ocean, 1989, 8(3):75-80) introduced 40 green abalones of 6-7cm in November 1985. Abalone, this batch of green abalone gave birth to offspring in multiple batches in January 1987. Due to the limitation of breeding technology at that time, due to the lack of suitable algae food, the offspring of green abalone self-propagation was seriously lost, so there was no follow-up report
Fabiola (Fabiola Lafarga de la Cruz, Cristian Gallardo-Escarate. Intraspecies and interspecies hybrids in Haliotis: natural and experimental evidence and its impact on abalone aquaculture. Reviews in Aquaculture, 2011, 3:74–99) on the current international The interspecific hybridization research of abalone has been reviewed. According to the literature, there is no report on the successful interspecific hybridization between green abalone and wrinkled pan abalone at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Green abalone and wrinkled pan abalone with good individual vitality and no muscle damage are selected as seed abalones. The average shell length of green abalone is 7.54cm, and the average shell length of wrinkled pan abalone is 8.32cm; the water temperature is controlled at 24°C through circulating water cooling facilities. Females and males were respectively placed in different parental abalone breeding ponds for breeding; after the gonads of the species abalone matured, the individuals with mature gonads were dried in the shade for 70 minutes, and then injected into seawater treated with ultraviolet light at an irradiation intensity of 1000mWh / L, and the seawater temperature was 22°C. Replace the ultraviolet-treated seawater every 60 minutes until the male and female abalones spawn and ejaculate. During the process of artificial induced labor, the male and female breeding abalones of green abalone and wrinkled pan abalone were placed in different containers, and the ...

Embodiment 2

[0020] Similar to Example 1, the difference is that the sperm of the green abalone and the eggs of the wrinkled abalone are mixed, and the concentration of the sperm of the wrinkled abalone is 2.58×10 6 pcs / ml, the result is green abalone ♀ × wrinkled plate abalone The hybrid fertilization rate was 18.32±3.12%. According to the conventional larvae, seedlings and intermediate breeding methods, the reciprocal abalone seedlings of green abalone and wrinkled abalone were finally obtained. The hybrid grows rapidly, has a high survival rate, is strong in high temperature resistance, has obvious heterosis, and has the potential to develop into a new abalone breeding variety.

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PUM

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Abstract

The invention relates to a seed production method by interspecific crossing of green abalone and Pacific abalone and relates to a hybrid seed production method for aquatic shellfishes. Pacific abalone and green abalone are used as parent abalones. Sea water temperature is controlled to 24 DEG C-26 DEG C through a temperature control facility to allow for synchronous accelerated ripening of gonads of the parent abalones. The male abalones and female abalones are placed in different culture ponds for breeding. Sex character of the parent abalones in each culture pond needs to be equal. The individuals with maturely developed gonads are induced for spawning by drying in the shape, ultraviolet irradiation and sea water stimulation until the female and male abalones lay eggs and discharge semen. Insemination is allowed at the water temperature of 20 DEG C to 22 DEG C. Inseminated hybrid abalone fry are obtained by direct cross combination, namely female Pacific abalone with green abalone, or by reciprocal cross combination, namely female abalone with Pacific abalone. The inseminated hybrid abalone fry are bred by conventional larva, offspring and intermediate breeding methods, and direct-cross or reciprocal-cross fry of green abalone and Pacific abalone are obtained finally.

Description

technical field [0001] The invention relates to a hybrid seed production method of shellfish aquatic organisms, in particular to a hybrid seed production method between green abalone and wrinkled pan abalone. Background technique [0002] Abalone is a kind of marine shellfish, known as the first of the eight seafood treasures, known as "soft gold", and has extremely high economic value. In recent years, the abalone breeding industry in Fujian Province has developed rapidly. In 2011, the output exceeded 60,000 tons, accounting for about 70% of the country's total, with an output value of nearly 10 billion yuan. The abalone breeding industry has become the fastest growing and most economically beneficial aquatic product pillar in Fujian Province. industry. [0003] Haliotis discus hannai is a cold-water species. It was originally the main abalone breeding object in northern my country, but after years of domestication in the south, it has become the most important abalone spe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 游伟伟柯才焕骆轩范飞龙
Owner 福建闽锐宝海洋生物科技有限公司
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