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Pig frozen semen basic diluent as well as preparation method and application thereof

A technology of frozen semen and diluent, which is applied in the field of porcine frozen semen diluent formula, achieving the effects of simple configuration, low cost and convenient operation

Active Publication Date: 2021-02-19
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At this stage, the research on the key technology of frozen production and preservation of pig semen has attracted the attention and attention of the global pig industry. Although the research on pig frozen semen in my country has made some breakthroughs in technology, there are also many studies reporting that pigs, especially local Frozen semen production of pig breeds still needs to be further improved technically

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A preparation method of the basic diluent of pig frozen semen is as follows: Weigh glucose, citric acid, Tris, cholesterol from pig liver, L-proline, and double distilled water, put them into a beaker and mix them, and heat to dissolve the above substances completely And cool down.

[0037] You can weigh 1.598g of glucose, 1.967g of citric acid, 3.52g of Tris, 0.483g of cholesterol from pig liver, 0.345g of L-proline, and 100ml of double distilled water.

[0038] A preparation method of pig frozen semen cryopreservation solution comprises: first weighing glucose, citric acid, Tris, pig liver source cholesterol, L-proline, double distilled water, putting them into a beaker and mixing, heating, so that the above-mentioned substances are completely After dissolving and cooling down, take the diluted solution and add 20% fresh egg yolk, penicillin and streptomycin, mix well, cool down to 4°C, let the sediment settle for 12 hours, take the supernatant and centrifuge at 3850 ...

Embodiment 2

[0050] The local pig breeds used in this example were Laiwu black pig, Dapulian pig and Licha black pig.

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PUM

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Abstract

The invention provides a pig frozen semen basic diluent. The basic diluent comprises glucose, citric acid, cholesterol, L-proline, Tris and double distilled water. A frozen pig semen cryopreservationliquid further comprises the following components in percentage by volume: 20% of fresh yolk, 1*10<6> IU / L of penicillin, 1*10<6> IU / L of streptomycin, 3%-4% of glycerinum and 1%-2% of Equex.STM. A pig fresh-keeping semen diluent further comprises isotonic skimmed milk. According to the invention, the survival rate of local pig fresh semen in the long-distance transportation process can be effectively maintained, and the damage of pig sperms in the freezing process is prevented or significantly reduced by adding pig liver-derived cholesterol, L-proline and an antifreeze agent Equex.STM; and through an effective thawing method, the operation is convenient, and the sperm motility and quality are improved.

Description

technical field [0001] The invention belongs to the technical field of agricultural biological breeding, and in particular relates to a formula of pig frozen semen diluent. Background technique [0002] Over the past few decades, due to the introduction and large-scale breeding of a large number of foreign pig breeds, the number of local pig breeds in my country has decreased sharply, and the number of bloodlines within the breed has also continued to decrease, and the genetic diversity has decreased, making many local pig breeds on the verge of extinction. In recent years, as the awareness of protecting local breed resources has increased, various measures have been taken to protect local pig breeds, and cryopreservation of boar semen is a convenient and effective means of preservation. Frozen semen is a more biosafe semen source. It can be stored in spare time to balance supply and demand. Due to the short storage time of fresh essence, the supply is in short supply duri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 张国梁宋俊霖
Owner QINGDAO AGRI UNIV
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