Preparation method of frozen bovine semen diluting liquid

Abstract

The invention discloses a preparation method of frozen bovine semen diluting liquid. The preparation method comprises the following steps: weighing 5g to 8g of fructose and 19.8g to 26.6g of sodium citrate; adding 800mL of distilled water to stir and filtering a solution with filter paper into a conical flask or a saline solution bottle; putting into a water-bath pot at 75 DEG C to 80 DEG C and disinfecting for 30min; cooling to room temperature and adding 180mL to 220mL of fresh yolk; adding 0.4 to 0.5 million IU units of penicillin and 0.4 to 0.5 million IU units of streptomycin, wherein a solution prepared according to the ratio is named as a first solution; adding 430mL of the first solution into 65mL to 75mL of glycerol; after stirring, putting a mixture into a refrigerator at 3 DEG C to 5 DEG C to obtain a second solution; then diluting semen, freezing and packaging. By adopting the preparation method, a fresh semen diluting effect and the live rate of sperm of excellent breeding bulls are improved, and frozen semen is effectively preserved for a long period.

Description

technical field [0001] The invention belongs to the technical field of animal husbandry and relates to a method for preparing a diluent of bovine frozen semen. Background technique [0002] The technology of bovine frozen semen and artificial insemination has been widely used in the production of cattle breeding in my country, so the production and quality control of bovine frozen semen has always been concerned by the industry. [0003] Practice has proved that the production of frozen semen is the core business of bull breeding stations in various places. The production process system should be established on the basis of standardized operating procedures, including cleaning and disinfection of equipment for producing frozen semen, dilution preparation, semen collection, semen processing, Semen freezing, thawing of frozen semen, microscopy and inspection rules of frozen semen, packaging of frozen semen, storage of frozen semen, and transportation of frozen semen. [0004]...

AI Technical Summary

Problems solved by technology

Most of the operators in each link have received vocational skills training and are highly standardized. The biggest difference is that the semen dilution solution is different, resulting in low sperm motility after dilution.

[0007] First of all, glucose and sucrose are not enough nutrients for the frozen semen diluent in bovine thin tubes. From the perspective of benefiting sperm metabolism and absorption, fructose should be chosen, because fructose has a simple structure and is more conducive to sperm metabolism and absorption under the same conditions. Sperm nutritional requirements; secondly, the amount of sodium citrate used to maintain the osmotic pressure balance of the internal and external environment of the sperm is not accurate enough to maintain the isotonicity of the solution and cause sperm deformation; thirdly, penicillin and streptomycin, as an antibacterial substance, were originally In order to improve the anti-pollution ability of sperm, if the amount is out of control, especially if the amount is too high, it will kill the sperm or reduce the vitality of the sperm; fourth, the freshness and disinfection of eggs are not strictly controlled, and the nutritional value is reduced; fifth, the amount of antifreeze glycerin is unreasonable , failed to achieve the corresponding antifreeze effect, but reduced the absorption of nutrients by sperm when the excess amount was exceeded; sixth, the operational details of bovine frozen semen production lacked standardization

[0008] In short, the dosage of each substance in the diluent formula is unreasonable. At the same time, it is necessary to further explore a more scientific production process of bovine thin tube frozen semen