Preparation method of frozen bovine semen diluting liquid

A technology for freezing semen and diluting liquid, applied in the field of animal husbandry and breeding, can solve the problems of reducing sperm motility, killing sperm, lack of rationality in dosage, and achieving the effect of improving the dilution effect.

Pending Publication Date: 2017-06-13
潘越博
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the operators in each link have received vocational skills training and are highly standardized. The biggest difference is that the semen dilution solution is different, resulting in low sperm motility after dilution.
[0007] First of all, glucose and sucrose are not enough nutrients for the frozen semen diluent in bovine thin tubes. From the perspective of benefiting sperm metabolism and absorption, fructose should be chosen, because fructose has a simple structure and is more conducive to sperm metabolism and absorption under the same conditions. Sperm nutritional requirements; secondly, the amount of sodium citrate used to maintain the osmotic pressure balance of the internal and external environment of the sperm is not accurate enough to maintain the isotonicity of the solution and cause sperm deformation; thirdly, penicillin and streptomycin, as an antibacterial substance, were originally In order to improve the anti-pollution ability of sperm, if the amount is out of control, especially if the amount is too high, it will kill the sperm or reduce the vitality of the sperm; fourth, the freshness and disinfection of eggs are not strictly controlled, and the nutritional value is reduced; fifth, the amount of antifreeze glycerin is unreasonable , failed to achieve the corresponding antifreeze effect, but reduced the absorption of nutrients by sperm when the excess amount was exceeded; sixth, the operational details of bovine frozen semen production lacked standardization
[0008] In short, the dosage of each substance in the diluent formula is unreasonable. At the same time, it is necessary to further explore a more scientific production process of bovine thin tube frozen semen

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Proportionally, accurately weigh 5 g of fructose and 26.6 g of sodium citrate into a 1000 mL beaker without loss, add 800 mL of distilled water, and stir with a sterilized glass rod or magnetic stirrer to fully dissolve the fructose and sodium citrate , then filter the solution with filter paper into a Erlenmeyer flask or a saline bottle, tie or stopper the bottle tightly, put it in a water bath at 75°C for 30 minutes, cool it down to room temperature, and then add 180 mL of fresh egg yolk, penicillin and streptomycin Each 400,000 IU unit, this solution is called Ⅰ solution; take 430 mL of Ⅰ solution and add 65 mL glycerin, and stir well with a magnetic stirrer, then put it in a 1°C refrigerator or biochemical incubator for use, and this solution is called Ⅱ liquid. It is worth noting that the prepared dilution should not be left for more than 24 hours.

[0065] Slowly add the first solution at 35°C to the original qualified semen, shake it gently, put an appropriate a...

Embodiment 2

[0067] Proportionally, accurately weigh 8 g of fructose and 26.6 g of sodium citrate into a 1000 mL beaker without loss, add 800 mL of distilled water, and stir with a sterilized glass rod or magnetic stirrer to fully dissolve the fructose and sodium citrate. Then filter the solution into a Erlenmeyer flask or a saline bottle with filter paper, tie or stopper the bottle tightly, put it in a water bath at 80°C for 30 minutes, and then add 220 mL of fresh egg yolk and each of penicillin and streptomycin after cooling to room temperature. 500,000 IU units, this solution is called Ⅰ solution; take 430 mL of Ⅰ solution and add 75 mL of glycerin, and stir well with a magnetic stirrer, then put it in a 4°C refrigerator or biochemical incubator for use, this solution is called Ⅱ liquid. It is worth noting that the prepared dilution should not be left for more than 24 hours.

[0068] Slowly add the first solution at 35°C to the original qualified semen, shake it gently, put an appropr...

Embodiment 3

[0070] Proportionally, accurately weigh 6 g of fructose and 23 g of sodium citrate into a 1000 mL beaker without loss, add 800 mL of distilled water, and stir with a sterilized glass rod or magnetic stirrer to fully dissolve the fructose and sodium citrate , and then filter the solution into a Erlenmeyer flask or a saline bottle with filter paper, tie or stopper the bottle tightly, put it in a water bath at 78°C for 30 minutes, and then add 200 mL of fresh egg yolk, penicillin and streptomycin after cooling to room temperature 450,000 IU units each, this solution is called Ⅰ solution; take 430 mL of Ⅰ solution and add 70 mL glycerin, and stir well with a magnetic stirrer, then put it in a refrigerator or a biochemical incubator at 2°C for use, and this solution is called Ⅱ liquid. It is worth noting that the prepared dilution should not be left for more than 24 hours.

[0071] Slowly add the first liquid at 35°C to the original qualified semen, shake it gently, put an appropr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method of frozen bovine semen diluting liquid. The preparation method comprises the following steps: weighing 5g to 8g of fructose and 19.8g to 26.6g of sodium citrate; adding 800mL of distilled water to stir and filtering a solution with filter paper into a conical flask or a saline solution bottle; putting into a water-bath pot at 75 DEG C to 80 DEG C and disinfecting for 30min; cooling to room temperature and adding 180mL to 220mL of fresh yolk; adding 0.4 to 0.5 million IU units of penicillin and 0.4 to 0.5 million IU units of streptomycin, wherein a solution prepared according to the ratio is named as a first solution; adding 430mL of the first solution into 65mL to 75mL of glycerol; after stirring, putting a mixture into a refrigerator at 3 DEG C to 5 DEG C to obtain a second solution; then diluting semen, freezing and packaging. By adopting the preparation method, a fresh semen diluting effect and the live rate of sperm of excellent breeding bulls are improved, and frozen semen is effectively preserved for a long period.

Description

technical field [0001] The invention belongs to the technical field of animal husbandry and relates to a method for preparing a diluent of bovine frozen semen. Background technique [0002] The technology of bovine frozen semen and artificial insemination has been widely used in the production of cattle breeding in my country, so the production and quality control of bovine frozen semen has always been concerned by the industry. [0003] Practice has proved that the production of frozen semen is the core business of bull breeding stations in various places. The production process system should be established on the basis of standardized operating procedures, including cleaning and disinfection of equipment for producing frozen semen, dilution preparation, semen collection, semen processing, Semen freezing, thawing of frozen semen, microscopy and inspection rules of frozen semen, packaging of frozen semen, storage of frozen semen, and transportation of frozen semen. [0004]...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 潘越博黄龙艳黄龙梅张登辉卢旺银贺军王玺年刘辉
Owner 潘越博
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap