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Method for lowering teratosperm ratio and dead sperm ratio in cattle and sheep sperm

A technology of deformed sperm and semen, applied in germ cells, vertebrate cells, animal cells, etc., to achieve the effect of improving sperm motility

Inactive Publication Date: 2014-04-09
内蒙古赛科星繁育生物技术(集团)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, density gradient centrifugation, filter column method, etc. are widely used to remove low-motility sperm for human sperm, and some results have been achieved after artificial insemination, but there are the following problems: 1. The kit is expensive and the cost is relatively high; 2. Repeated centrifugation It may cause physical damage to sperm, and the quality of semen will decrease after freezing; 3. It is difficult to completely remove the residue of the kit, and whether it will affect the follow-up of conception needs to be confirmed by experiments

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] NaHCO 3 10mmol / L,

[0057] Hepes 40mmol / L,

[0058] MgCl 2 0.4mmol / L,

[0059] NaLactate 25mmol / L,

[0060] KCl 3mmol / L,

[0061] Glucose 5mmol / L,

[0062] NaCl 95mmol / L,

[0063] Na 2 HPO 4 0.3mmol / L,

[0064] NaPyruvate 2mmol / L,

[0065] 0.3%BSA 3g / L;

[0066] Dissolve the above components into ultrapure water in turn, and make the final volume to 1L;

[0067] 2. Storage of Staining Talp dilution:

[0068] The Staining Talp dilution is stored at 4°C, and the shelf life is two weeks;

[0069] 3. Upstream processing of bovine fresh semen:

[0070] The experimental semen came from the bull of Inner Mongolia Saikexing Breeding Biotechnology Co., Ltd., code-named 15507212, and the semen was collected by the false vagina method;

[0071] (1) Preheat the container at 37°C in advance, then preheat 4ml of Staining Talp diluent to 37°C, and place it in the container;

[0072] (2) Select fresh bovine semen with a vitality range of 40%-60%, that is, a dead sper...

Embodiment 2

[0106] NaHCO 3 10mmol / L,

[0107] Hepes 40mmol / L,

[0108] MgCl 2 0.4mmol / L,

[0109] NaLactate 25mmol / L,

[0110] KCl 3mmol / L,

[0111] Glucose 5mmol / L,

[0112] NaCl 95mmol / L,

[0113] Na 2 HPO 4 0.3mmol / L,

[0114] NaPyruvate 2mmol / L,

[0115] 0.3%BSA 3g / L;

[0116] Dissolve the above components into ultrapure water in turn, and make the final volume to 1L;

[0117] 2. Storage of Staining Talp dilution:

[0118] The Staining Talp dilution is stored at 4°C, and the shelf life is two weeks;

[0119] 3. Upstream processing of sheep fresh semen:

[0120] The experimental semen is from the ram of Inner Mongolia Saikexing Breeding Biotechnology Co., Ltd., code-named R025, and the semen is collected by the false vagina method;

[0121] (1) Preheat the container at 37°C in advance, then preheat 4ml of Staining Talp diluent to 37°C, and place it in the container;

[0122] (2) Select fresh goat semen with a vitality range of 40%-60%, that is, a dead sperm rate of...

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Abstract

The invention relates to a method for lowering teratosperm ratio and dead sperm ratio in cattle and sheep sperm, which comprises the following steps: preparing a Staining Talp dilute solution from NaHCO3, Hepes, MgCl2, NaLactate, KCl, Glucose, NaCl, Na2HPO4, NaPyruvate and 0.3% BSA (bovine serum albumin), preheating the Staining Talp dilute solution to 37 DEG C, and putting into a container; injecting fresh sperm with the activity interval of 40-60% to the bottom of the Staining Talp dilute solution, and treating in a 37 DEG C warm bath; and since normal live sperm swims upwards and is on the supernatant of the dilute solution, absorbing the supernatant, and centrifugating at 3000 rpm / minute to collecting the sperm. The teratosperm ratio and dead sperm ratio in the cattle and sheep sperm treated by the upward swimming process are obviously lowered; and the method can enhance the X / Y sperm separation efficiency, enhance the sperm activity and increase the fertility rate and conception rate.

Description

technical field [0001] The invention belongs to the technical field of animal reproduction, in particular to a method for reducing the ratio of deformed sperm and dead sperm in bovine and sheep semen. Background technique [0002] Mammalian semen with high deformity rate and low motility will lead to low conception rate. For example, my country has formulated the "National Standard for Bovine Frozen Semen". %; sperm motility after thawing frozen semen ≥ 35%, sperm deformity rate ≤ 18%. At present, density gradient centrifugation, filter column method, etc. are widely used to remove low-motility sperm for human sperm, and some results have been achieved after artificial insemination, but there are the following problems: 1. The kit is expensive and the cost is relatively high; 2. Repeated centrifugation It may cause physical damage to sperm, and the quality of semen will decrease after freezing; 3. It is difficult to completely remove the residue of the kit, and whether it ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/076
Inventor 丁瑞周文忠韩红梅李喜和胡树香
Owner 内蒙古赛科星繁育生物技术(集团)股份有限公司
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