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Method for lowering teratosperm ratio and dead sperm ratio in cattle and sheep sperm
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A technology of deformed sperm and semen, applied in germ cells, vertebrate cells, animal cells, etc., to achieve the effect of improving sperm motility
Inactive Publication Date: 2014-04-09
内蒙古赛科星繁育生物技术(集团)股份有限公司
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Problems solved by technology
At present, density gradientcentrifugation, filter column method, etc. are widely used to remove low-motility sperm for human sperm, and some results have been achieved after artificial insemination, but there are the following problems: 1. The kit is expensive and the cost is relatively high; 2. Repeated centrifugation It may cause physical damage to sperm, and the quality of semen will decrease after freezing; 3. It is difficult to completely remove the residue of the kit, and whether it will affect the follow-up of conception needs to be confirmed by experiments
Method used
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[0070] The experimental semen came from the bull of Inner Mongolia Saikexing Breeding Biotechnology Co., Ltd., code-named 15507212, and the semen was collected by the false vagina method;
[0071] (1) Preheat the container at 37°C in advance, then preheat 4ml of Staining Talp diluent to 37°C, and place it in the container;
[0072] (2) Select fresh bovine semen with a vitality range of 40%-60%, that is, a dead sper...
[0120] The experimental semen is from the ram of Inner Mongolia Saikexing Breeding Biotechnology Co., Ltd., code-named R025, and the semen is collected by the false vagina method;
[0121] (1) Preheat the container at 37°C in advance, then preheat 4ml of Staining Talp diluent to 37°C, and place it in the container;
[0122] (2) Select fresh goat semen with a vitality range of 40%-60%, that is, a dead sperm rate of...
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Abstract
The invention relates to a method for lowering teratosperm ratio and dead sperm ratio in cattle and sheep sperm, which comprises the following steps: preparing a Staining Talp dilute solution from NaHCO3, Hepes, MgCl2, NaLactate, KCl, Glucose, NaCl, Na2HPO4, NaPyruvate and 0.3% BSA (bovine serum albumin), preheating the Staining Talp dilute solution to 37 DEG C, and putting into a container; injecting fresh sperm with the activity interval of 40-60% to the bottom of the Staining Talp dilute solution, and treating in a 37 DEG C warm bath; and since normal live sperm swims upwards and is on the supernatant of the dilute solution, absorbing the supernatant, and centrifugating at 3000 rpm / minute to collecting the sperm. The teratosperm ratio and dead sperm ratio in the cattle and sheep sperm treated by the upward swimming process are obviously lowered; and the method can enhance the X / Y sperm separation efficiency, enhance the sperm activity and increase the fertility rate and conception rate.
Description
technical field [0001] The invention belongs to the technical field of animal reproduction, in particular to a method for reducing the ratio of deformed sperm and dead sperm in bovine and sheep semen. Background technique [0002] Mammalian semen with high deformity rate and low motility will lead to low conception rate. For example, my country has formulated the "National Standard for Bovine Frozen Semen". %; sperm motility after thawing frozen semen ≥ 35%, sperm deformity rate ≤ 18%. At present, density gradientcentrifugation, filter column method, etc. are widely used to remove low-motility sperm for human sperm, and some results have been achieved after artificial insemination, but there are the following problems: 1. The kit is expensive and the cost is relatively high; 2. Repeated centrifugation It may cause physical damage to sperm, and the quality of semen will decrease after freezing; 3. It is difficult to completely remove the residue of the kit, and whether it ...
Claims
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Application Information
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