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Livestock semen normal-temperature preservative and preparation method and use method thereof

A preservative and semen technology, applied in the field of livestock semen preservatives at room temperature, can solve the problems of low sperm motility and sperm integrity, harsh cryopreservation conditions, and high workload of staff, and achieves easy large-scale popularization and application, reduces temperature limitations, and the effect of improving work efficiency

Active Publication Date: 2020-09-15
张良斌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, after thawing semen stored at low temperature, the activity and integrity rate of sperm are lower, which has a great impact on the later fertilization rate
Moreover, the conditions for cryopreservation are relatively harsh, usually at 4°C. If you need to carry a refrigerator for insemination, it is very inconvenient, the work intensity of the staff is relatively high, and the cost is relatively high.

Method used

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  • Livestock semen normal-temperature preservative and preparation method and use method thereof
  • Livestock semen normal-temperature preservative and preparation method and use method thereof
  • Livestock semen normal-temperature preservative and preparation method and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The screening of embodiment 1 egg yolk dosage

[0033] Set up 5 groups of test groups, which are respectively recorded as groups A1, A2, A3, A4, and A5. The dosages of egg yolk in groups A1, A2, A3, A4, and A5 are 5mL, 10mL, 15mL, 20mL, and 25mL, respectively. Same, prepare the preservative as follows:

[0034] (1) Take 6g of Tris, 1.8g of sodium citrate, 0.45g of vitamin C, 0.05g of glutathione, 0.17g of bovine serum albumin, 0.09g of ATP, 0.06g of penicillin, and 0.06g of streptomycin g, 1.75g ​​of trehalose was added to 20mL of double distilled water, and stirred evenly with a glass rod.

[0035] (2) Beat the eggs into a clean beaker (petri dishes and other utensils that can hold eggs are acceptable, and the eggs must not be muddy), insert a syringe without a needle into the egg yolk to absorb the egg yolk liquid and add it to the liquid prepared in the previous step.

[0036] (3) Add the solution prepared in step (2) into double distilled water to prepare a 100ml ...

Embodiment 2

[0041] Example 2 Screening of trehalose dosage

[0042] Set up 5 test groups, which are respectively recorded as B1, B2, B3, B4, B5 groups, and the amount of trehalose in B1, B2, B3, B4, B5 groups are 0.5g, 1.0g, 1.5g, 2.0g, 2.5g g, the remaining components are used in the same amount, and the preservative is prepared as follows:

[0043] (1) Take 6g of Tris, 1.8g of sodium citrate, 0.45g of vitamin C, 0.05g of glutathione, 0.17g of bovine serum albumin, 0.09g of ATP, 0.06g of penicillin, and 0.06g of streptomycin g, add 20 mL of double distilled water to trehalose, and stir evenly with a glass rod.

[0044] (2) Beat the eggs into a clean beaker (petri dishes and other utensils that can hold eggs are acceptable, and the eggs must not be muddy), insert a syringe without a needle into the egg yolk, absorb 15mL of egg yolk liquid and add it to the liquid prepared in the previous step.

[0045] (3) Add the solution prepared in step (2) into double distilled water to prepare a 10...

Embodiment 3

[0052] (1) Take 6g of Tris, 1.8g of sodium citrate, 0.45g of vitamin C, 0.05g of glutathione, 0.17g of bovine serum albumin, 0.09g of ATP, 0.06g of penicillin, and 0.06g of streptomycin g, add 20 mL of double-distilled water to 2 g of trehalose, and stir evenly with a glass rod.

[0053] (2) Beat the eggs into a clean beaker (petri dishes and other utensils that can hold eggs, the eggs must not be teased), insert a syringe without a needle (capacity greater than 10ml) into the egg yolk, absorb 10ml of egg yolk liquid and add it to the prepared in the previous step in liquid.

[0054] (3) Add the solution prepared in step (2) into double distilled water to prepare a 100ml solution, stir and mix with a glass rod to obtain the livestock semen room temperature preservative of the present invention.

[0055] When it needs to be used, prepare the preservative in advance according to the requirements. When the preservative is not in use, it should be stored in the refrigerator at 4°...

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Abstract

The invention discloses a livestock semen normal-temperature preservative and a preparation method and a use method thereof. Every 100 mL of double distilled water contains the following components: 5-7 g of tris(hydroxyl)aminomethane (Tris), 1.6-2.0 g of sodium citrate, 0.4-0.5 g of vitamin C, 0.04-0.06 g of glutathione, 0.16-0.18 g of bovine serum albumin, 0.08-0.09 g of adenosine triphosphate,0.1-0.14 g of antibiotics, 5-20 ml of egg yolk and 0.5-2.5 g of trehalose. By using the livestock semen normal-temperature preservative, fresh semen can be stored at 15 to 25 DEG C, the cost of carrying refrigeration appliances such as a liquid nitrogen tank and a refrigerator is reduced, working efficiency of technicians is effectively improved, the preservation time is 72 hours or longer, the 72-hour survival rate is 45% or higher, the conception rate is 40% or higher after two times of continuous insemination, artificial insemination within the range of 300 kilometers can be guaranteed, thepreparation process is simple, the production cost is low, and large-scale application and popularization are facilitated.

Description

technical field [0001] The invention relates to the field of livestock semen preservation agent, in particular to a livestock semen preservation agent at room temperature. Background technique [0002] With the development of animal husbandry industrialization, the production of high-quality mutton sheep is to meet the needs of the international market. However, the mutton sheep industry in my country started relatively late and has not yet formed a breeding system for mutton sheep. Most of the breeds are local breeds with poor meat production performance. The quality is also lower, and the market competitiveness is weaker. Since the 1980s, my country's sheep industry has successively introduced high-quality mutton sheep breeds from abroad to improve local sheep in my country, improve their meat production rate and mutton quality, and have made great contributions to improving the quality and meat of my country's sheep. [0003] At present, my country has introduced high-qua...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226A01N1/0215A01N1/0221
Inventor 张良斌柴局张仙保贺心康刘自运布鲁木图王永光张新杰高振江王雪峰赵启南刘自增李彦欣王亮明杜金丽
Owner 张良斌
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