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Preservation method of animal fresh semen and fresh semen preserving diluent specially used by preservation method

A preservation method and diluent technology, applied in the biological field, can solve problems such as increased embryo mortality, reduced conception rate, shortened sperm survival time, etc., and achieve the effect of important application value

Inactive Publication Date: 2017-06-20
张志刚 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the extension of storage time, the motility of semen decreases, the integrity of the plasma membrane of sperm is destroyed, and the survival time of sperm in the reproductive tract of ewes is also shortened, which leads to the increase of embryo mortality and the decrease of conception rate

Method used

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  • Preservation method of animal fresh semen and fresh semen preserving diluent specially used by preservation method
  • Preservation method of animal fresh semen and fresh semen preserving diluent specially used by preservation method
  • Preservation method of animal fresh semen and fresh semen preserving diluent specially used by preservation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, the preparation of fresh essence preservation diluent

[0034] According to the formula in Table 1, fresh essence storage dilution A was prepared. According to the formula in Table 2, fresh essence storage dilution B was prepared. According to the formula in Table 3, fresh essence storage dilution C was prepared.

[0035] Table 1

[0036] Reagent Dosage for preparing 78mL fresh essence preservation diluent A Concentration in Fresh Semen Preservation Diluent A Tris 2.97g 38mg / mL penicillin 40,000 units 0.051 million units / mL streptomycin 45,000 units 0.058 million units / mL citric acid 2.00g 26mg / mL glucose 0.72g 9mg / mL Vitamin B12 0.027g 0.35mg / mL glycerin 7.0mL 0.09mL / mL water 71.0mL /

[0037] Note: " / " means it does not exist.

[0038] Table 2

[0039]

[0040]

[0041] Note: " / " means it does not exist.

[0042] table 3

[0043] Reagent The amount ...

Embodiment 2

[0045] The application of the fresh essence preservation diluent prepared in embodiment 2 and embodiment 1 in preservation of sheep essence

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Abstract

The invention discloses a preservation method of animal fresh semen and fresh semen preserving diluent specially used by the preservation method. The preservation method of the animal fresh semen, which is provided by the invention, comprises the following step of preserving the animal fresh semen by adopting the fresh semen preserving diluent; the fresh semen preserving diluent is prepared from 38 to 47mg / mL Tris, 510 to 640 units / mL penicillin, 580 to 710 units / mL streptomycin, 26 to 38mg / mL citric acid, 9 to 11mg / mL glucose, 0.35 to 0.42mg / mL vitamin B12 and 0.09 to 0.11mL / mL glycerinum. Experiments prove that by the method provided by the invention, the fresh semen of a breeding ram can be preserved for 24 to 96h, then insemination is performed on ewes, the conception rate of the ewes is between 83.5% and 86.2%, and the conception rate is not significantly different from the natural mating conception rate of the ewes. Therefore, by adopting the method provided the invention, the animal fresh semen can be preserved, and the preservation method has important application value.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preserving animal fresh essence and a special fresh essence preservation diluent thereof. Background technique [0002] Artificial insemination is a powerful means for efficient multiplication of fine sheep populations, and the quality of semen determines the success or failure of artificial insemination techniques. At present, most sheep farms in China use low-temperature storage to store fresh sheep essence for a short period of time, and the effective storage time of fresh essence is only 1-2 days. With the prolongation of storage time, the motility of semen decreases, the integrity of sperm plasma membrane is destroyed, and the survival time of sperm in the reproductive tract of ewes is also shortened, which leads to the increase of embryo mortality and the decrease of conception rate. Therefore, how to effectively preserve sheep's fresh essence plays an important r...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0226
Inventor 张志刚任俊光
Owner 张志刚
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