Experimental method for preserving diluent of sheep semen under room temperature

Abstract

The invention discloses an experimental method for preserving a diluent of sheep semen under a room temperature. The experimental method comprises the following specific steps: 1) extracting the fresh sheep semen; 2) putting a semen collecting cup into a thermal insulation barrel and returning the semen collecting cup to a laboratory; 4) preparing four diluents; 5) putting the diluents into a 37-DEG C water-bath pot; 6) diluting the fresh semen; 7), storing diluted semen I, II, III and IV obtained in the step 6), tightly covering a centrifugal tube cover, putting the centrifugal tube on a foamed centrifugal tube rack, and placing and storing under the room temperature; 8), measuring a sperm motility rate in diluted semen; and 10), detecting a sperm plasma membrane integrity rate in the diluted semen. According to the experimental method for preserving the diluent of the sheep semen under the room temperature, disclosed by the invention, the semen with a relatively high survival rate is extracted, and influences, on experimental results, of a low sperm survival rate are reduced; and survival rates of sheep sperms are separately detected through the four groups of diluents with different proportions, and the measured diluent proportion of the diluent III is more beneficial for survival of the sperms.

Description

technical field [0001] The invention relates to the technical field of an experimental method for storing a diluent of sheep semen at room temperature, in particular to an experimental method for storing a diluent of sheep semen at room temperature. Background technique [0002] Since the invention of the sheep semen preservation technology, it has become one of the most effective ways to improve the utilization rate of high-quality breeding stock and accelerate the improvement of varieties, and it can also reduce the cost of reproduction. The popularization and application of artificial insemination technology has greatly promoted the development of the sheep industry. . The storage methods of semen are divided into cryopreservation, cryopreservation and normal temperature preservation. [0003] Cryopreservation is for the preservation of variety resources and the exchange of germplasm between regions, and can be maintained for a long time; low temperature preservation (0-...

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide an experimental method for storing diluents of sheep semen at room temperature, so as to solve the problem that the components of the diluents proposed in the above-mentioned background technology are mostly complicated and numerous, and the preservation effect is not very ideal, and the preservation time is generally 5 to 72 Hours, reducing the utilization rate of fine rams and increasing the cost of raising sheep

Images