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Ultra-high activity tubule semen freezing method for sheep

A freezing method and sheep thin tube technology, which is applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of unstable freezing effect, low viability and vitality of sheep frozen semen, and achieve optimal pH value and Effects of osmotic pressure, resolution of low viability and vitality, and improvement of pregnancy rate

Inactive Publication Date: 2012-09-12
SHANXI AGRI UNIV
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AI Technical Summary

Problems solved by technology

[0003] In order to solve the problems of low viability and vitality and unstable freezing effect of existing frozen sheep semen, the present invention provides a method for freezing sheep thin tube semen with ultra-high vitality

Method used

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Examples

Experimental program
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Embodiment Construction

[0018] A method for freezing ultra-high vitality goat straw semen, comprising the following steps:

[0019] (1) High motility screening of goat sperm before freezing:

[0020] First prepare the cleaning solution: add 21.0 parts of potassium chloride (KCl), 653.0 parts of sodium chloride (NaCl), sodium dihydrogen phosphate monohydrate (NaH 2 PO 4 ·H 2 O) 6.2 parts, sodium bicarbonate (NaHCO 3 ) 209.0 parts, sodium lactate (C 3 h 5 NaO 3 ) 11.0 parts, magnesium chloride hexahydrate (MgCl 2 ·6H 2 O) 9.0 parts, Hepes buffer salt 122.0 parts, sodium propionate (C 3 h 5 NaO 2 ) 10.5 parts, glucose (C 6 h 12 o 6 ) 255.0 parts, bovine serum albumin 600 parts and mixed evenly to obtain the cleaning solution; put the cleaning solution into the first sterile centrifuge tube and store in CO 2 Pre-equilibrate in the incubator for 2 hours;

[0021] from CO 2 Take out the first sterile centrifuge tube from the incubator, use a straw to suck out the goat semen from the s...

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Abstract

The invention relates to a semen freezing method for sheep, in particular to an ultra-high activity tubule semen freezing method for sheep. The method solves the problems that after the conventional frozen semen of sheep thaws, the motility rate and the activity are low, and the frozen effect is unstable. The method comprises the following steps of: filling fresh semen of sheep into the bottom part of a first centrifuging tube holding cleaning liquid; standing in a CO2 incubator for 30 to 60 minutes, wherein the semen with high activity goes up in supernatant; sucking the supernatant and thenfilling into a second centrifuging tube for centrifugation, wherein the semen with high density and high activity is obtained at the bottom of the second centrifuging tube; and sucking the condensed high-activity semen, adding into a frozen diluent, uniformly mixing and filling in tubules; performing program freezing and storing in liquid nitrogen, and thawing the stored semen before semen deposition. The sheep semen is subjected to high activity screening before freezing according to the method, and the motility rate of the thawing tubule frozen semen of sheep reaches 0.45 to 0.60, requirements on the artificial fertilization of the frozen semen of sheep are fully met, and the method can be widely applied to mass production of sheep.

Description

technical field [0001] The invention relates to a method for freezing sheep semen, in particular to a method for freezing ultra-high vitality sheep capillary semen. Background technique [0002] The study of sheep frozen semen began in the 1930s abroad and in 1978 in my country, but so far no mature and stable programmed freezing technology has been formed. Sheep sperm are more fragile than chicken and bovine sperm, and are easily damaged during the freezing and thawing process, thus losing their fertilization ability; although the sperm used for freezing requires a liveness rate of more than 0.8 before freezing, but due to individual differences in different rams The motility of the sperm produced varies, and the effect of sperm freezing is very unstable. At the same time, the existing sheep frozen semen has a viability of less than 0.35 after thawing and there are many "senile" sperm and weak dead sperm, with few effective sperm, low vitality and poor quality. Frozen she...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 张建新白元生杨子森刘晓妮师周戈张春香任有蛇焦光月
Owner SHANXI AGRI UNIV
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