1461 results about "Centrifugation Tube" patented technology

The invention provides a method for extracting carbon quantum dots from activated carbon, which comprises the following steps of: adding dry activated carbon powder to salpeter solution and stirring for backflow; performing reduced pressure distillation for evaporating suspension obtained by backflow to dryness; dispersing obtained black solids in water, and neutralizing obtained solution with sodium hydroxide; and finally, centrifugating neutralized black suspension for removing precipitation, separating supernatant fluid by using an ultrafiltration centrifugal tube or an ultrafiltration membrane, collecting filtrate, and drying the filtrate to obtain carbon quantum dots. The method uses cheap and available activated carbon as carbon sources, and can obtain a large number of carbon quantum dots by simple chemical oxidation process and simple subsequent processes of evaporation, saturation, centrifugation and ultrafiltration. The carbon quantum dots are graphite structure nanocrystals with the grain diameter of 3 to 5 nm, the surfaces of which have a large amount of hydroxide radicals. The carbon quantum dots have good fluorescence and electrochemiluminescence.

The invention discloses a coaxial centrifugal spinning device and method. The device comprises a direct-current motor, a supporting device, a spinning support, coaxial centrifuge tubes, spinning needle heads and a receiving device, wherein the direct-current motor is fixed to the supporting device, the spinning support is directly and fixedly connected with an output shaft of the direct-current motor, the coaxial centrifuge tubes are fixed to the spinning support, and under the action of the direct-current motor, the spinning support drives the coaxial centrifuge tubes to rotate at high speed; each coaxial centrifuge tube comprises an outer layer centrifuge tube and an inner layer centrifuge tube, each spinning needle head comprises an outer layer needle head and an inner layer needle head, during spinning, a spinning solution or melt in the outer layer centrifuge tubes and the inner layer centrifuge tubes is tossed out under the action of centrifuge force, an outer layer solution wraps an inner layer solution, and superfine fiber of a skin-core structure is formed due to centrifuge force drafting, solvent volatilization and phase separation. The coaxial centrifugal spinning device is simple, high in spinning speed and production efficiency and capable of producing coaxial superfine fiber on a large scale.

A fluid aspirating/dispensing member includes a sample cavity for sample acquisition and a sealable cavity that, once sealed, permits the separation of particles from the remainder of a fluid sample within the sample cavity after centrifugation or other separation means. The fluid aspirating/dispensing members, either individually or as part of an array, increase the efficiency of sample processing before analysis by a clinical analyzer.

The invention relates to the field of analytical chemistry and food safety, and provides a high performance liquid chromatography detection method for residual quantity of multiple ultraviolet absorbents in cosmetics. A cosmetic sample of 0.4g is weighed and placed in a plastic centrifuge tube of 50 ml, sample extraction liquid of 20ml is added, after the cosmetic sample of 0.4g and the sample extraction liquid of 20ml are mixed for 2 minutes in a vortex mode, sonic oscillation extraction is carried out for 20 minutes, 8000 revolutions per minute centrifugation is carried out for 10 minutes and, and supernate is to be purified; and after test solutions sequentially pass through microfiltration membranes of 0.45 micrometer and 0.20 micrometer, a high performance liquid chromatograph is adopted to carry out qualitative determination and quantitative assay. The detection method has the advantages of being simple, fast, accurate, efficient, and the like, and can detect 12 ultraviolet absorbents at the same time. Technical indexes of detection minimum, the recovery rate, the degree of precision, and the like all meet relevant demands for ultraviolet absorbent detection in sun block cosmetics at home and abroad. The method is suitable for detection and monitoring of the ultraviolet absorbents in the sun block cosmetics, and has great significance on promoting of import and export trade of cosmetics of our country, ensuring of safety of the cosmetics, and guarantee of human body health.

The invention discloses an extraction and purification method of total plant endophyte genome DNA for colony analysis. The method comprises the following steps of: shearing and grinding the surface bacteria removed fresh plant tissues into paste, soaking the plant tissues into sterilized phosphate buffer solution, performing thermostatic shaking for 1 hour in a table concentrator to separate out microbial cells from the plant tissues, standing, transferring the suspension to a centrifuge tube, performing centrifugal collection on microbial bacteria, then adding lysing solution, shaking and uniformly mixing the solution to release DNA from the cells, repeatedly extracting protein by chloroform-isoamylol, performing centrifugal sedimentation with isopropanol and washing with 70 percent ice ethanol, performing purification by using a DNA specific centrifugal adsorption column to obtain total crude extracted genome DNA, and finally, performing polymerase chain reaction (PCR) amplificationby using special primers to obtain high-quality plant endophyte specific DNA fragments for subsequent analysis. The invention has the advantages that: the method is simple and effective, high in quality, low in pollution and the like, and provides high-quality guarantee for comprehensively and completely researching a plant endophyte colony structure.

A system for extracting and processing adipose tissue to generate a therapeutically effective amount of adipose-derived stem cells, comprising an adipose tissue extraction device and a modified centrifuge tube comprising a plurality of lipoaspirate inlet fittings, a plurality of processing fluid inlet fittings, and a plurality of pellet extraction tubes. The adipose tissue extraction device is used to extract a quantity of adipose tissue from a human being, the lipoaspirate is moved into the first modified centrifuge tube via a sterile transfer, a plurality of processing steps are performed to clean and dissociate the lipoaspirate, and a pellet containing an enhanced fraction of stem cells is obtained by centrifuging the modified centrifugal tube. The pellet is resuspended in a fluid and administered to a human patient for a therapeutic or cosmetic purpose.

The invention relates to a method for quickly determining the content of additive of a plurality of types in food. An adopted technical scheme is as follows: a solid sample is accurately weighed into a centrifuge tube, petroleum ether or n-hexane is additionally arranged to become homogenate, and supernatant fluid is removed by centrifugation; after the petroleum ether or the n-hexane in the residue are volatilized, the mixed solvent of ethanol, ammonia and water is additionally arranged, ultrasonic extraction is carried out, and the supernatant fluid is obtained by centrifugation as sample liquid for testing; the sample liquid for testing is adopted, potassium ferrocyanide solution and zinc acetate solution are additionally arranged, the mixture is mixed uniformly and centrifuged; the supernatant fluid is concentrated in water bath at 60DEG C to 100DEG C; concentrated solution is transferred to a measuring flask with water; the pH value is regulated to 5 to 7, water is additionally arranged to fix the capacity to a scale; the mixture is mixed uniformly, is filtered by a 0.45mum microfiltration membrane and arranged in a sample bottle as sample liquid; and the sample liquid is tested in a liquid chromatograph by gradient elution under variable wavelengths. According to the invention, the method for quickly determining the content of additive of a plurality of types in food is simple and easy, has high precision, high accuracy, high sensitivity, has the recovery rate being above 80 percent, and is used for determining the content of additive of a plurality of types in food at the same time.

The invention provides a preparation method for a chlorella polypeptide microcapsule. The method comprises the following steps: extracting chlorella protein by using a low temperature ultrahigh pressure continuous flow cell crusher; hydrolyzing the chlorella protein with papain; filtering the hydrolyzed chlorella protein with an ultrafiltration centrifuge tube; then carrying out separation and purification by using ion exchange chromatography DEAE-52 and gel chromatography dextrangel G-25 columns; and finally utilizing complex coacervation to prepare the chlorella polypeptide microcapsule. The chlorella polypeptide microcapsule prepared in the invention has antitumor activity, e.g., the chlorella polypeptide microcapsule has inhibitory effects on in vitro growth of human hepatoma carcinoma cells HepG2 and the inhibition rate of the chlorella polypeptide microcapsule reaches 38% when concentration is 400 mu g/mL. Therefore, the chlorella polypeptide microcapsule prepared in the invention is beneficial for development and utilization of antitumor health food and medicinal products.

The invention discloses a preparation method of a hydrogen evolution electric catalyst based on a metal-organic framework compound. The method includes the following steps that 1, cupric acetate aquo-complex Cu (OAc) <2>H<2>O is dissolved in acetic acid for preparing a cupric acetate solution; 2, trimesic acid (H<3>BTC) particles are dissolved in absolute ethyl alcohol for preparing a trimesic acid solution; 3, the trimesic acid solution is transferred to and mixed with the cupric acetate solution; 4, the mixed solution is subjected to ultrasonic operation; 5, the mixed solution is placed in a centrifugal tube for centrifugal operation and activating treatment; 6, a centrifugal product obtained after activating treatment is placed in a drying oven for being dried; 7, after samples and organic solvent are mixed according to proportion, the Cu-MOF@Nafion hydrogen evolution catalyst is obtained. The obtained Cu-MOF@Nafion hydrogen evolution catalyst has unique physical properties of good transmission protons, good electrochemical stability is achieved, efficiency of a hydrogen evolution reaction can be improved, and the recycling service life can be greatly prolonged.

The invention relates to an extraction method of platelet rich plasma (PRP) and extracted PRP and particularly relates to a method for extracting PRP from blood. The method comprises the following steps of: (a) putting the collected whole blood in a container containing an anticoagulant, and fully and uniformly mixing the blood and the anticoagulant; (b) putting the blood mixed with the anticoagulant in a centrifuge tube, carrying out primary centrifugation, and enabling the blood to be divided into three layers; (c) extracting the uppermost layer and most of the middle layer, transferring the uppermost layer and most of the middle layer into a new centrifuge tube, mixing uniformly and carrying out secondary centrifugation; and (d) discarding the plasma at the upper layer of the centrifuge tube, and resuspending the precipitated plasma by utilizing the remaining plasma, thus obtaining the PRP. The method provided by the invention has the advantages that the operation process is simple, the yield is high and the concentration of platelet in rich plasma is high.

The invention relates to a method for detecting 153 pesticide residues in a heart benefiting and pulse restoring particle. The method is a liquid chromatography-mass spectrometry method. The method comprises the following steps: preparing a sample solution, preparing a matrix mixed reference substance working solution, and detecting the solutions through using the high performance liquid chromatography-mass spectrometry, wherein the sample solution is prepared through the following steps: crushing the heart benefiting and pulse restoring particle, weighing 1.8-2.2 g of the heart benefiting and pulse restoring particle, adding 80-120 [mu]L of an internal standard solution with the concentration of 5 [mu]g/mL, adding 8-12 mL of water, infiltrating the particle for 28-32 min, adding 8-12 mL of an acetonitrile solution containing 0.08-0.12% of acetic acid, oscillating the obtained solution in a swirl mixing oscillator at a rate of 4000-6000 r/min for 1.8-2.2 min, adding 3-5 g of anhydrous magnesium sulfate and 0.08-1.2 g of sodium acetate, carrying out swirl shaking for 4-6 min, centrifuging the obtained solution at a speed of 4000-6000 r/min for 4-6 min, taking 0.8-1.2 mL of the obtained supernatant, adding the supernatant to a centrifuging tube which is filled with 140-460 mg of anhydrous sodium sulfate, 23-27 mg of PSA, 48-52 mg of C18 and 2.3-2.7 mg of GCB in advance, carrying out swirl shaking for 2-4 min, centrifuging the obtained solution at a rate of 4000-6000 r/min for 4-6 min, taking the obtained supernatant, and filtering the supernatant by a 0.2-0.24 [mu]m filter membrane.

An analysis method for a trace tobacco specific N-nitrosamine (TSNAs) in an animal blood sample is characterized by comprising the following steps of: placing the animal blood sample into a centrifuge tube with ethylene diamine tetraacetic acid EDTA-k2 saturated aqueous solution, performing the centrifugation at 10,000 rpm; adding the upper blood plasma into the mixed internal standard solution of NNN-d4, NNK-d4, NAT-d4, NAB-d4 and NNAL-d3; after standing for 30min to precipitate protein, and filtering upper solution; and detecting the content of the tobacco specific N-nitrosamine in the sample by adopting LC-MS/MS. The method has the advantages of exactly determining the content of the tobacco specific N-nitrosamine (TSNAs) and the content of metabolites thereof in the complex matrix sample of the animal blood. Compared with the prior art, the invention has the characteristics of simplicity in operation, high sensitivity and reliability in result, and creates the method of determining the content of the tobacco specific N-nitrosamine (TSNAs) and the content of the metabolites thereof in the complex matrix sample of the animal blood.

The invention relates to a semen freezing method for sheep, in particular to an ultra-high activity tubule semen freezing method for sheep. The method solves the problems that after the conventional frozen semen of sheep thaws, the motility rate and the activity are low, and the frozen effect is unstable. The method comprises the following steps of: filling fresh semen of sheep into the bottom part of a first centrifuging tube holding cleaning liquid; standing in a CO2 incubator for 30 to 60 minutes, wherein the semen with high activity goes up in supernatant; sucking the supernatant and thenfilling into a second centrifuging tube for centrifugation, wherein the semen with high density and high activity is obtained at the bottom of the second centrifuging tube; and sucking the condensed high-activity semen, adding into a frozen diluent, uniformly mixing and filling in tubules; performing program freezing and storing in liquid nitrogen, and thawing the stored semen before semen deposition. The sheep semen is subjected to high activity screening before freezing according to the method, and the motility rate of the thawing tubule frozen semen of sheep reaches 0.45 to 0.60, requirements on the artificial fertilization of the frozen semen of sheep are fully met, and the method can be widely applied to mass production of sheep.

The invention provides a manufacturing method for the oblique rolling of a bimetallic compound seamless steel pipe by a centrifugal blank, which comprises the following steps: producing a bimetallic compound circular pipe blank on a centrifugal pipe die by adopting a centrifugal casting process and pushing a pipe when the temperature of an inner-layer metal layer is 950 to 1400 DEG C; carrying out heat treatment on the bimetallic compound circular pipe blank according to the purpose of the bimetallic compound circular pipe; carrying out machining or surface oxidation treatment on the pipe blank according to the purpose after rolling; rolling the bimetallic compound circular pipe blank by adopting an oblique rolling machine set; and carrying out the heat treatment on the bimetallic compound circular pipe blank after being rolled. According to the manufacturing method for the oblique rolling of the bimetallic compound seamless steel pipe by the centrifugal blank in the production of an oblique rolling process, which is adopted by the invention, crystal grains are thinned by the centrifugal blank through the deformation of each passage of the oblique rolling machine set, and the integral performance of the seamless steel pipe is enhanced. Meanwhile, the bonding strength between two layers of metal of the bimetallic compound seamless steel pipe with the oblique rolling of the centrifugal blank is ensured by adopting the centrifugal blank and the oblique rolling process, the processing cost is also lowered, and the metal yield is enhanced.

The invention relates to a preparation method and application of a temperature-responded silver nanocluster/polymer hydrogel composite material, relates to a preparation method and application of a silver nanocluster/polymer hydrogel composite material, and aims to solve the problems that an existing method for detecting hexavalent chromium ions needs to depend on a large analyzing tester or sample preparation is difficult, detection time is long and the like. The method comprises the following steps: firstly, dissolving weighed temperature-sensitive polymeric monomers A in deionized water, adding polymeric monomers B, mechanically stirring, heating, adding an initiator, and reacting under protection of nitrogen to obtain a solution; secondly, placing the solution in a centrifugal tube, carrying out centrifuging, and taking supernate after centrifuging to obtain a polymeric hydrogel nanoparticle solution; and thirdly, weighing silver nitrate, adding the silver nitrate in the polymeric hydrogel nanoparticle solution, and carrying out photoreduction under ultraviolet light to obtain the temperature-responded silver nanocluster/polymer hydrogel composite material. The preparation method is used for preparing fluorescent metal nanoclusters.

The invention discloses a simple nucleic acid simplifying method which comprises processes of cracking, adsorption promotion, washing and elution. The method is characterized by comprising preparation methods and use methods of a simple purifying column, cracking liquid, adsorption promoting liquid and washing liquid special for nucleic acid purification, wherein the simple purifying column is a 1.5 ml centrifuge tube with a hole on the bottom; a small mass of absorbent cotton is compacted on the tube bottom; 0.3-1.0 g of glass powder with diameter of 120-130 mum is added as a purifying column body; an uncovered 2.0 ml centrifuge tube is sleeved outside the column body to serve as a collection tube, therefore, the collection tube is easily manufactured by using a common EP (Eppendorf) tube, cotton and glass powder. With a wide application range, the method is directly applied to the extraction and purification of genome DNA, plasmid DNA and total RNA, as well as the recycling, centrifugation and purification of PCR (polymerase chain reaction) products; the operation steps are concise and easy to master, and the requirements on the experiment operation technique are not high; moreover, the method has the advantages of high purification purity, high integrity, short purification time and low experiment cost; and as toxic substances such as phenol and chloroform are not used in the purification process, the method is environmentally friendly and energy-saving.

An automated process for converting samples includes: receiving tube strips having a number of sample tubes and samples therein, transferring multiple tube strips to a tube strip holder, dispensing sample conversion buffer into each tube, shaking the tube strip holder a first time, centrifuging the tube strip holder, removing a liquid supernatant from each tube, simultaneously inspecting the contents of each tube, dispensing a specimen transport medium and a denaturation reagent into each tube, shaking the tube strip holder a second time, heating the tube strip holder for a first length of time, shaking the tube strip holder a third time, heating the tube strip holder for a second length of time, shaking the tube strip holder a fourth time, and transferring at least a portion of each sample to a respective well on an output plate.

The invention discloses a rapid detection method for nitrites in blood and urine. The method comprises: utilizing a diazotization coupling reaction of nitrites and Griess reagent to form an azo compound, performing cloud point extraction, precipitating the colored azo compound at the bottom of a centrifuge tube, comparing the red color at the tube bottom with standard color gradation, and determining the content of the nitrite according to the color depth. Because the enriching multiple of cloud point extraction is large and blood and urine are subjected to decoloring and protein-removal processing, the interference is substantially eliminated, and the detection limit of nitrites in the system can reach 0.01 mu g/mL. The method has the characteristics of simpleness, rapidness, high sensitivity, on-site detection and the like.

The invention discloses a single-filtration seminal fluid separating centrifuge tube, which comprises a sleeve, wherein a filtering pipe is arranged in the sleeve; the outer wall of the upper part of the sleeve has threads; the upper part of the filtering pipe is cylindrical and the lower part of the filtering pipe is shaped like a truncated cone; the bottom end of the filtering pipe is connected with a conduit with a diameter smaller than that of the filtering pipe; the filtering pipe is communicated with the conduit; a buffering net is arranged on the upper end of the truncated cone of the filter pipe; the outer wall of the buffering net is in tight fit with the inner wall of the upper end of the truncated cone; upper, middle and lower three layers of polyester fiber filtering membranes are arranged between the buffering net and a supporting net frame; through holes are formed on the filtering membranes uniformly; and a first supporting ring, a second supporting ring and a third supporting ring are matched with the inner wall of the truncated cone respectively. In the invention, a purely physical method is used to filter a seminal fluid sample which has a liquefaction abnormal, so fibrin filaments, gel particles and cell components in the seminal fluid are removed, the seminal fluid is completely liquefied, the sperms move freely, and part of sperms with special shapes and part of dead sperms can be removed at the same time.

The invention discloses a method for detecting benzopyrene in edible oil. The method comprises the following steps of weighing an oil sample; adding a potassium hydroxide ethanol water solution; vibrating a mixture until the mixture is clarified; saponifying the clarified mixture in a water bath at the temperature of 50 DEG C for 1 hour; cooling the mixture until the temperature decreases to room temperature, and adding water into a sample centrifugation tube; after the mixture and water are shaken uniformly, adding 15ml of normal hexane, performing uniform mixing and centrifugation, and washing two times by 5ml of ultrapure water; concentrating an extraction solution in a nitrogen blowing concentrator until the extraction solution is dried completely; making up to constant volume through a mixed solution consisting of 20 percent of tetrahydrofuran and 80 percent of acetonitrile; and performing filtration through a filtering membrane, and then determining a result through a high performance liquid chromatograph. The analysis method disclosed by the invention has the advantages of simplicity in operation, high stability and high recovery rate.

The invention relates to a platelet-rich plasma rapid separating device and a method, and belongs to the field of platelet-rich plasma extracting devices and methods. The device comprises a centrifugal tube; the centrifugal tube comprises an inner tube body, an outer tube body and a sealing cover, wherein the inner tube body is sleeved with the sealing cover; the inner tube body is provided with a shrinking channel, blood is centrifuged, then suction of cell components can be avoided in the mode that the channel is closed through a buckling device, the centrifugal operation procedure is simplified, and the concentration and the preparing efficiency of the platelet-rich plasma after centrifugation are increased; meanwhile, the outer tube body of the centrifugal tube is in a centrifugal-tube shape, the centrifugal tube can be in fit with a universal centrifugal device in the market, and a platelet-rich plasma enrichment device is easy to operate, low in cost and capable of providing a high-concentration and low-cost platelet-rich-plasma and platelet-rich-gel device and method for clinical research.

The invention discloses a simple method for extracting and determining peanut fatty acids, which comprises the following steps: carrying out freeze-drying on a fresh sample to obtain dry tissues; weighing the dry sample, putting into a mortar, and grinding and pulverizing with liquid nitrogen; or putting the dry sample into a jaw-top empty bottle, and crushing with a glass bar; adding reagents I and II, evenly mixing by using ultrasonic, adding a reagent III, optionally adding an internal standard, and carrying out water bath reaction; after the reaction is finished, cooling, adding an organic reagent and a salt solution, evenly mixing, sucking out the solution, transferring into a centrifuge tube, and standing; carrying out centrifugal separation, and absorbing the supernate for machine determination; and calculating the content of the fatty acid component by a normalization process or internal standard process. The method simplifies the sample pretreatment process, is simple and quick to operate, greatly shortens the testing time, has the advantages of low cost, low quantity of required samples and favorable universality, and is a simple and accurate method for extracting and measuring peanut fatty acids in the laboratory.

The invention discloses a method for determining the residue of triazine pesticide in tea by dispersive liquid-liquid micro-extraction and magnetic solid-phase extraction. The method is a tea sample pretreatment method. The tea sample pretreatment method comprises the following steps of: putting 0.5 g of sample into a 10ml polypropylene centrifugal tube with a stopper, adding 3 ml of acetonitrile, shaking, centrifuging, moving supernatant to another centrifugal tube, adding 3 ml of acetonitrile into residue, repeatedly executing the steps, and combining extracting solutions which are obtained at two times; drying by nitrogen, moving 5 ml of water, whirling, adding 20 microliters of n-octyl alcohol, shaking, adding 200 microliters and 20 mg/ml of magnetic nano particle solution, shaking, attracting magnetic nano particles to the wall of the centrifugal tube by using a magnet, and removing effluent; and adding 50 microliters of methanol into the centrifugal tube, whirling, attracting the magnetic nano particles to the bottom of the centrifugal tube by using the magnet, taking the solution out by using a microsyringe, and performing efficient liquid chromatography-mass spectrometry analysis. The method is easy and convenient to operate, short in analysis time, low in cost, high in sensitivity and applicable to detection of pesticide residue of the tea.

The invention belongs to the blood product technology field, and discloses a preparation method for leukocyte-depleted platelet rich plasma. The method comprises the following steps: firstly, anticoagulants and blood are drawn and mixed, and whole blood to be separated is obtained; secondly, first centrifugation is carried out, a centrifugate divided into three layers from bottom to top is obtained, and platelet and plasma components in the upper layer are obtained; thirdly, the obtained upper layer components are centrifuged again, and platelet deposition at the bottom and a plasma supernatant are formed; part of the plasma supernatant is drawn, the platelet deposition is subjected to resuspension by the left plasma supernatant, and leukocyte-depleted platelet rich plasma is prepared. The method achieves elimination of leukocytes and enrichment of platelets at the same time, operation is convenient, consumed time is short, the technical indexes of the prepared plasma product are better than technical indexes of present products with the same kind. Centrifugation for two times in the method can be carried out in two centrifuge tubes respectively, or be carried out in one centrifugal device. The invention provides a centrifugal device used for performing the preparation method.