Platelet-rich plasma rapid separating device and method

A platelet-rich plasma and platelet-rich technology, applied in chemical instruments and methods, centrifuges, laboratory equipment, etc., can solve the problems of limiting the clinical application of platelet-rich plasma, slow enrichment speed of platelet-rich plasma, high preparation cost, etc. Achieve the effects of increasing concentration and preparation efficiency, solving long preparation cycle and low cost

Active Publication Date: 2017-04-26
SHENZHEN DAKEWE BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the preparation devices of platelet-rich plasma at home and abroad are based on density gradient centrifugation, buoy centrifugal enrichment, multi-bag separation, etc. Because the sterility of platelet-rich plasma must be maintained, these devices are generally complicated in structure and high in preparation costs. And it needs the supporting centrifuge device to assist in the separation
[0006] At present, the platelet-rich plasma preparation kits produced on the market are separated by cen...

Method used

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  • Platelet-rich plasma rapid separating device and method
  • Platelet-rich plasma rapid separating device and method

Examples

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Effect test

Embodiment 1

[0064] A device for rapidly extracting high-concentration platelet-rich plasma, including a centrifuge tube, the centrifuge tube includes an inner tube body, an outer tube body and a sealing cover sleeved on the inner tube body, and the upper part of the inner tube body in sealing contact with the sealing cover is a hard It is made of high-quality polymer materials, and the rest is made of soft polymer materials. There is a contraction channel in the middle, and the inner tube body and the outer tube body are set together; the contraction channel is equipped with a buckle device, which can close the contraction channel. The outer tube body is a conical centrifugal tube made of hard polymer material; the top surface of the sealing cover body is provided with a syringe needle penetrable area, and the side lower edge of the sealing cover body and the upper edge of the outer tube body are in the inner tube body external connections.

[0065] The inner tube body is made of polyethy...

Embodiment 2

[0083] The method for one-step separation and preparation of low-concentration platelet-rich plasma using a device for rapidly extracting high-concentration platelet-rich plasma is characterized by comprising the following steps:

[0084] A. Collect 25ml of peripheral blood from volunteers for anticoagulation;

[0085] B. Add the blood sample into a 50ml centrifuge tube with a flat needle syringe;

[0086] C. Put the centrifuge tube into the centrifuge and centrifuge for 10 minutes at a centrifugal force of 1000g. The blood is divided into three layers: the upper layer is the plasma layer, the middle layer is the platelet layer, and the lower layer is the red blood cell layer;

[0087] D. If the buckle is a split type buckle, lift the centrifuge tube with the cover out of the inner tube, use the buckle to close the contraction channel at the interface between plasma and red blood cells, and put the inner tube back into the outer tube; if the buckle is integrated Buckle, then ...

Embodiment 3

[0090] The method for two-step separation and preparation of high-concentration platelet-rich plasma using a device for rapidly extracting high-concentration platelet-rich plasma is characterized by comprising the following steps:

[0091] A. Collect 25ml of peripheral blood from volunteers for anticoagulation;

[0092] B. Add the blood sample into a 50ml centrifuge tube with a flat needle syringe;

[0093] C. Put the centrifuge tube into the centrifuge and centrifuge for 10 minutes at a centrifugal force of 1000g. The blood is divided into three layers. The upper layer is the plasma layer, the middle layer is the platelet layer, and the lower layer is the red blood cell layer;

[0094] D. If the buckle is a split type buckle, lift the centrifuge tube with the cover out of the inner tube, use the buckle to close the contraction channel at the interface between plasma and red blood cells, and put the inner tube back into the outer tube; if the buckle is integrated Buckle, then...

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Abstract

The invention relates to a platelet-rich plasma rapid separating device and a method, and belongs to the field of platelet-rich plasma extracting devices and methods. The device comprises a centrifugal tube; the centrifugal tube comprises an inner tube body, an outer tube body and a sealing cover, wherein the inner tube body is sleeved with the sealing cover; the inner tube body is provided with a shrinking channel, blood is centrifuged, then suction of cell components can be avoided in the mode that the channel is closed through a buckling device, the centrifugal operation procedure is simplified, and the concentration and the preparing efficiency of the platelet-rich plasma after centrifugation are increased; meanwhile, the outer tube body of the centrifugal tube is in a centrifugal-tube shape, the centrifugal tube can be in fit with a universal centrifugal device in the market, and a platelet-rich plasma enrichment device is easy to operate, low in cost and capable of providing a high-concentration and low-cost platelet-rich-plasma and platelet-rich-gel device and method for clinical research.

Description

[0001] 【Technical field】 [0002] The invention relates to a device and method for rapidly extracting platelet-rich plasma, and belongs to the field of platelet-rich plasma extraction equipment and methods. [0003] 【Background technique】 [0004] Platelet rich plasma (platelet rich plasma, PRP) is the platelet concentrate obtained by centrifugation and separation of whole blood, which is rich in platelets. When platelets are activated, they can release a variety of growth factors, which play an important role in promoting the proliferation, growth, differentiation and tissue formation of bone cells and osteoblasts. Since Marx et al first used PRP composite bone graft to repair mandibular defects in 1998, PRP has been gradually used in tissue repair in the fields of stomatology, plastic surgery, orthopedics, ENT, neurosurgery and other fields, as well as medical cosmetology and hair loss treatment. and anti-aging fields. [0005] At present, the preparation devices of platele...

Claims

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Application Information

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IPC IPC(8): B01L3/14B04B7/00
Inventor 姜维吴庆军
Owner SHENZHEN DAKEWE BIOENG
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