Preparation method for chlorella polypeptide microcapsule

A technology of chlorella peptides and microcapsules, which is applied in peptide preparation methods, food preparation, chemical instruments and methods, etc., can solve the problems of tumor cell drug resistance, low treatment efficiency, and large toxic and side effects, and achieve beneficial The effect of development and utilization

Inactive Publication Date: 2013-01-02
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Malignant tumors are one of the diseases that seriously threaten human health and life. Although the existing anti-tumor drugs have certain curative effects on most tum...

Method used

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  • Preparation method for chlorella polypeptide microcapsule
  • Preparation method for chlorella polypeptide microcapsule
  • Preparation method for chlorella polypeptide microcapsule

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Embodiment 1

[0029] The preparation method of chlorella polypeptide microcapsules, the steps are as follows:

[0030] (1) Chlorella protein was extracted using a low-temperature ultrahigh-pressure continuous flow cell crusher: Mix chlorella powder and pure water at a mass ratio of 1:20 and stir for 50 minutes to obtain a mixed solution. Extract the chlorella protein from the above mixed solution at a temperature of 6°C and a pressure of 100 MPa, and obtain the crude extract as the next extraction solution for re-extraction. The number of extractions is 3 times, and the time for each extraction is 20min. Finally, the obtained solution was centrifuged at a temperature of 4 °C and a speed of 6000 r / min for 30 min to remove the precipitate to obtain a protein supernatant, and then rapidly freeze-dried in a vacuum to obtain a chlorella protein powder.

[0031] (2) Based on the chlorella protein powder obtained in step (1), prepare a chlorella protein solution with a concentration of 2%, and ad...

Embodiment 2

[0037] The preparation method of chlorella polypeptide microcapsules, the steps are as follows:

[0038] (1) Chlorella protein was extracted using a low-temperature ultrahigh-pressure continuous flow cell crusher: Mix chlorella powder and pure water at a mass ratio of 1:30 and stir for 80 minutes to obtain a mixed solution. Extract the chlorella protein from the above mixed solution at a temperature of 8°C and a pressure of 150 MPa, and obtain the crude extract as the next extraction solution for re-extraction. The number of extractions is 6 times, and the time for each extraction is 50min. Finally, the obtained solution was centrifuged at a temperature of 4 °C and a speed of 5000 r / min for 20 min to remove the precipitate to obtain a protein supernatant, and then rapidly freeze-dried in a vacuum to obtain a chlorella protein powder.

[0039] (2) Based on the chlorella protein powder obtained in step (1), configure a chlorella protein solution with a concentration of 2%, and ...

Embodiment 3

[0043] The preparation method of chlorella polypeptide microcapsules, the steps are as follows:

[0044] (1) Chlorella protein was extracted using a low-temperature ultra-high pressure continuous flow cell disruptor: Mix chlorella powder and pure water at a mass ratio of 1:35 and stir for 100 minutes to obtain a mixed solution. Extract the chlorella protein from the above mixed solution at a temperature of 9°C and a pressure of 200 MPa, and obtain the crude extract as the next extraction solution for re-extraction. The number of extractions is 2 times, and the time for each extraction is 10min. Finally, the obtained solution was centrifuged at 4 °C and 8000 r / min for 30 min to remove the precipitate to obtain the protein supernatant, and then quickly freeze-dried in vacuum to obtain the chlorella protein powder.

[0045] (2) Based on the chlorella protein powder obtained in step (1), prepare a chlorella protein solution with a concentration of 2%, and add papain for hydrolysi...

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Abstract

The invention provides a preparation method for a chlorella polypeptide microcapsule. The method comprises the following steps: extracting chlorella protein by using a low temperature ultrahigh pressure continuous flow cell crusher; hydrolyzing the chlorella protein with papain; filtering the hydrolyzed chlorella protein with an ultrafiltration centrifuge tube; then carrying out separation and purification by using ion exchange chromatography DEAE-52 and gel chromatography dextrangel G-25 columns; and finally utilizing complex coacervation to prepare the chlorella polypeptide microcapsule. The chlorella polypeptide microcapsule prepared in the invention has antitumor activity, e.g., the chlorella polypeptide microcapsule has inhibitory effects on in vitro growth of human hepatoma carcinoma cells HepG2 and the inhibition rate of the chlorella polypeptide microcapsule reaches 38% when concentration is 400 mu g/mL. Therefore, the chlorella polypeptide microcapsule prepared in the invention is beneficial for development and utilization of antitumor health food and medicinal products.

Description

technical field [0001] The invention relates to a preparation method of chlorella polypeptide microcapsules, which can be applied to functional food and medicine, and belongs to the field of chlorella deep processing and biotechnology. Background technique [0002] Chlorella pyenoidosa is a kind of universal single-celled green algae, which belongs to Chlorophyta and the genus Chlorella. It grows fast, is easy to cultivate, and has high application value. As a new type of health food and medicine, it has great potential. Chlorella has anti-tumor activity, increases immunity, detoxifies and protects the liver, and lowers blood pressure. Its crude protein content is high (about 50%) and its quality is good. It has become a very active and highly valued application field of chlorella. aspect. [0003] Bioactive peptides refer to those peptides with special physiological activity or functional properties. The vast majority of proteins are precursors of biologically active pep...

Claims

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Application Information

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IPC IPC(8): A61K38/01A61K9/52A61P35/00A61P1/16A23L1/29C12P21/06C07K1/18C07K1/16A23L33/00
Inventor 张学武王晓琴
Owner SOUTH CHINA UNIV OF TECH
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