High performance liquid chromatography detection method for residual quantity of multiple ultraviolet absorbents in cosmetics

A high-performance liquid chromatography and ultraviolet absorber technology is applied in the field of detection of residual ultraviolet absorbers in cosmetics, and can solve problems such as the lack of a method for detecting multiple ultraviolet absorbers at the same time.

Inactive Publication Date: 2013-04-24
林维宣
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High-performance liquid chromatography has the characteristics of relatively simple pretreatment and relatively high sensitivity, and is widely used, but most of them can only detect several ultraviolet absorbers,

Method used

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  • High performance liquid chromatography detection method for residual quantity of multiple ultraviolet absorbents in cosmetics
  • High performance liquid chromatography detection method for residual quantity of multiple ultraviolet absorbents in cosmetics
  • High performance liquid chromatography detection method for residual quantity of multiple ultraviolet absorbents in cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Detection method of residual amount of various ultraviolet absorbers in ointment sample (cream)

[0044] 1. Extraction of samples

[0045] Weigh 0.4 g (accurate to 0.01 g) of cosmetic samples into a 50 mL plastic centrifuge tube, add 20 mL of sample extract, vortex and mix for 2 minutes, extract by ultrasonic oscillation for 20 minutes, centrifuge at 8000r / min for 10 minutes, and the supernatant To be purified.

[0046] 2. Purification

[0047] After the test solution passes through 0.45 μm and 0.20 μm microporous membranes in sequence, it is to be tested on the machine.

[0048] 3. Determination by high performance liquid chromatography

[0049] According to the content of UV absorber in the sample, select the standard working solution with similar response value to carry out HPLC determination together, inject the standard working solution and sample liquid with equal volumes, and measure the UV absorption in the standard working solution and sample liqui...

Embodiment 2

[0078] Example 2 Detection method of residual amount of multiple ultraviolet absorbers in emulsion

[0079] 1. Sample extraction: Same as Example 1.

[0080] 2. Purification: same as example 1.

[0081] 3. Determination by high performance liquid chromatography: with example 1.

[0082] 4. Linear relationship test: Same as Example 1.

[0083] 5. Determination of lower limit of detection: same as Example 1.

[0084] 6. Recovery rate and precision: 3 different levels of 12 kinds of ultraviolet absorbers were added to the milky blank sample, and the addition levels are shown in Table 5. 6 parallel experiments were performed at each level. Analyze as above. The calculation results of recovery rate and coefficient of variation are shown in Table 5.

[0085] The recoveries and relative standard deviations (n=6) of 12 kinds of ultraviolet absorbers in table 5 milky samples

[0086]

[0087] It can be seen from the results in Table 5 that this method detects milky cosmetics:...

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Abstract

The invention relates to the field of analytical chemistry and food safety, and provides a high performance liquid chromatography detection method for residual quantity of multiple ultraviolet absorbents in cosmetics. A cosmetic sample of 0.4g is weighed and placed in a plastic centrifuge tube of 50 ml, sample extraction liquid of 20ml is added, after the cosmetic sample of 0.4g and the sample extraction liquid of 20ml are mixed for 2 minutes in a vortex mode, sonic oscillation extraction is carried out for 20 minutes, 8000 revolutions per minute centrifugation is carried out for 10 minutes and, and supernate is to be purified; and after test solutions sequentially pass through microfiltration membranes of 0.45 micrometer and 0.20 micrometer, a high performance liquid chromatograph is adopted to carry out qualitative determination and quantitative assay. The detection method has the advantages of being simple, fast, accurate, efficient, and the like, and can detect 12 ultraviolet absorbents at the same time. Technical indexes of detection minimum, the recovery rate, the degree of precision, and the like all meet relevant demands for ultraviolet absorbent detection in sun block cosmetics at home and abroad. The method is suitable for detection and monitoring of the ultraviolet absorbents in the sun block cosmetics, and has great significance on promoting of import and export trade of cosmetics of our country, ensuring of safety of the cosmetics, and guarantee of human body health.

Description

technical field [0001] The invention relates to the fields of analytical chemistry and food safety, in particular to a method for detecting the residual amount of ultraviolet absorbers in cosmetics. Background technique [0002] In recent years, the amount of ultraviolet radiation has been increasing along with the destruction of the ozone layer, and the impact on human beings has been recognized worldwide. At present, ultraviolet absorbers with safe and efficient absorption of ultraviolet radiation have been widely used in cosmetics, hair care products, fabric treatment and detergents to protect the human body from excessive ultraviolet radiation. [0003] The mechanism of action of UV Absorbent is based on its intramolecular hydrogen bond. The intramolecular hydrogen bond formed between the hydroxyl hydrogen on the benzene ring and the adjacent carbonyl oxygen forms a chelate ring. After absorbing ultraviolet light, the molecule undergoes thermal vibration, the hydrogen b...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/08
Inventor 林维宣孙兴权马杰谢琰于灵
Owner 林维宣
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