Single-filtration seminal fluid separating centrifuge tube

Abstract

The invention discloses a single-filtration seminal fluid separating centrifuge tube, which comprises a sleeve, wherein a filtering pipe is arranged in the sleeve; the outer wall of the upper part of the sleeve has threads; the upper part of the filtering pipe is cylindrical and the lower part of the filtering pipe is shaped like a truncated cone; the bottom end of the filtering pipe is connected with a conduit with a diameter smaller than that of the filtering pipe; the filtering pipe is communicated with the conduit; a buffering net is arranged on the upper end of the truncated cone of the filter pipe; the outer wall of the buffering net is in tight fit with the inner wall of the upper end of the truncated cone; upper, middle and lower three layers of polyester fiber filtering membranes are arranged between the buffering net and a supporting net frame; through holes are formed on the filtering membranes uniformly; and a first supporting ring, a second supporting ring and a third supporting ring are matched with the inner wall of the truncated cone respectively. In the invention, a purely physical method is used to filter a seminal fluid sample which has a liquefaction abnormal, so fibrin filaments, gel particles and cell components in the seminal fluid are removed, the seminal fluid is completely liquefied, the sperms move freely, and part of sperms with special shapes and part of dead sperms can be removed at the same time.

Description

technical field [0001] The invention relates to a semen separation device, which is a disposable filter type semen separation centrifuge tube. Background technique [0002] Non-liquefaction of semen will hinder sperm swimming, which will eventually lead to infertility. Men with infertility due to non-liquefied semen account for about 7%-8% of infertility. At present, the treatment of these infertile patients generally uses assisted reproductive technology to achieve better results. However, an important prerequisite for assisted reproductive technology is how to obtain a large number of high-quality sperm from fresh semen. At present, semen samples with abnormal liquefaction are difficult to optimize in vitro. Even if there are enough high-quality sperm, it is difficult to effectively separate them. For this reason, those skilled in the art add enzyme preparations to semen samples with abnormal liquefaction to promote their liquefaction, and then perform intrauterine artif...

AI Technical Summary

Problems solved by technology

However, an important prerequisite for assisted reproductive technology is how to obtain a large number of high-quality sperm from fresh semen. At present, semen samples with abnormal liquefaction are difficult to optimize in vitro. Even if there are enough high-quality sperm, it is difficult to effectively separate them. For this reason, those skilled in the art add enzyme preparations to semen samples with abnormal liquefaction to promote their liquefaction, wash them before performing intrauterine artificial insemination

Although this method is simple and easy to operate, the PH value of the semen will change after adding enzyme preparations to the semen, which will easily reduce the survival rate and vitality of the sperm, and cause damage to the structure of the sperm, especially the sperm membrane, resulting in a lower pregnancy rate. At the same time, It is difficult to control the amount of enzyme preparation added

In order to solve the above problems and increase the pregnancy rate, in recent years, those skilled in the art have devoted themselves to studying the use of purely physical methods to separate abnormally liquefied semen, so as to achieve the purpose of separating and obtaining high-quality sperm. Some devices that have been reported, although used for separating Sperm in semen, however, due to different uses, its structure is complex, and the separation process is complicated to operate, which affects the vitality of sperm, and it is still difficult to increase the pregnancy rate

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