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A kind of Hu sheep semen cryopreservation solution and its application

A cryopreservation solution and cryopreservation technology, which is applied in the field of Hu sheep breeding, can solve the problems such as difficult to meet artificial insemination, few effective sperm, and poor quality, so as to improve the survival rate, stabilize the conception effect, and meet the requirements of artificial insemination Effect

Active Publication Date: 2020-12-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing sheep frozen semen has a low viability after thawing and there are many "aging" sperm and weak dead sperm. The effective sperm is less, the vitality is low, and the quality is poor. It is difficult to meet the needs of artificial insemination.

Method used

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  • A kind of Hu sheep semen cryopreservation solution and its application

Examples

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Effect test

Embodiment 1

[0021] An embodiment of the Hu sheep semen cryopreservation solution of the present invention, the Hu sheep semen cryopreservation solution contains components of the following concentrations: Tris 30g / L, glucose 10g / L, raffinose 40g / L, malt oil 3% (v / v), sodium citrate 18g / L, glycerol 5% (v / v), cryoprotectant 0.1g / L, penicillin 1,000,000 IU / L and streptomycin 1g / L; Described cryoprotectant contains astragalus extract, acerola cherry extract and bamboo leaf extract, and the mass ratio of the astragalus extract, acerola cherry extract and bamboo leaf extract is 2:1:1.

[0022] The preparation method of the Hu sheep semen cryopreservation solution comprises the following steps: (1) Weighing Tris, glucose, raffinose, sodium citrate and cryoprotectant in proportion, adding double distilled water, fully dissolving and mixing uniformly, Filter with a 0.2um filter membrane, then add penicillin and streptomycin, mix well to get solution A; (2) add malt oil to solution A in proportion,...

Embodiment 2

[0024] An embodiment of the Hu sheep semen cryopreservation solution of the present invention, the Hu sheep semen cryopreservation solution comprises the following concentrations of components: Tris 33g / L, glucose 15g / L, raffinose 30g / L, malt oil 5% (v / v), sodium citrate 20g / L, glycerin 6% (v / v), cryoprotectant 0.06g / L, penicillin 1,000,000 IU / L and streptomycin 1g / L; Described cryoprotectant contains astragalus extract, acerola cherry extract and bamboo leaf extract, and the mass ratio of the astragalus extract, acerola cherry extract and bamboo leaf extract is 2:1:1.

[0025] The preparation method of the Hu sheep semen cryopreservation solution is the same as that in Example 1.

Embodiment 3

[0027] An embodiment of the Hu sheep semen cryopreservation solution of the present invention, the Hu sheep semen cryopreservation solution contains the following components: Tris 35g / L, glucose 12g / L, raffinose 35g / L, malt oil 2% (v / v), sodium citrate 16g / L, glycerol 8% (v / v), cryoprotectant 0.13g / L, penicillin 800,000 IU / L and streptomycin 0.8g / L; It comprises astragalus extract, acerola cherry extract and bamboo leaf extract, and the mass ratio of the astragalus extract, acerola cherry extract and bamboo leaf extract is 2:1:1.

[0028] The preparation method of the Hu sheep semen cryopreservation solution is the same as that in Example 1.

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Abstract

The invention discloses a Hu sheep semen cryopreservation solution, which comprises the following components: Tris, glucose, raffinose, malt oil, sodium citrate, glycerin, cryoprotectant, penicillin and streptomycin; the cryoprotectant Herbs include Astragalus Extract, Acerola Cherry Extract, and Bamboo Leaf Extract. The invention also discloses the preparation method and application of the Hu sheep semen cryopreservation solution. The cryopreservation solution provided by the present invention can effectively improve the survival rate, vigor and integrity of the Hu sheep semen during the preservation process, and the preserved semen still has good insemination ability after thawing, and the conception effect is stable, which can meet the requirements of artificial Hu sheep frozen semen. Insemination requirements, broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of Hu sheep breeding, and in particular relates to a Hu sheep semen cryopreservation solution and its application. Background technique [0002] Hu sheep is one of the important livestock in the Taihu Plain, and it is a first-class protected local livestock breed in my country. Hu sheep have medium physique, male and female have no horns, long and narrow head, protruding nose bridge, large and drooping ears, long and thin neck, long and narrow body, straight back and waist, slightly drooping abdomen, flat and round tail with upturned tail tip, and partial limbs. Thin and tall. The coat is all white, the belly hair is thick, thin and short, and the body is strong. Hu sheep is a rare breed of white lambskin sheep with excellent traits such as early maturity, estrus in four seasons, two litters a year, multiple lambs per litter, good lactation performance, fast growth and development, ideal meat production pe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 刘莉君邱寒峰彭彩娥王邵雨王争光
Owner ZHEJIANG UNIV
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