Diluent of frozen semen of milk goat, and preparation method and diluting method thereof as well as preparation method of frozen semen of milk goat in thin pipe

A technology for freezing diluent and freezing semen is applied in the biological field and can solve the problems of difficulty in improving the conception rate and large-scale production, affecting the integrity of the plasma membrane, sperm motility rate, fertilization ability, and changes.

Inactive Publication Date: 2014-01-22
NORTHWEST A & F UNIV
View PDF8 Cites 22 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of frozen semen technology and artificial insemination technology in dairy cows and beef cattle in my country has been relatively well developed, which has fully improved the economic benefits of breeding. However, although goat semen cryopreservation and artificial insemination technology have been used for many years, the frozen semen is generally frozen after thawing. , the sperm motility rate (0.3-0.5) is difficult to increase the conception rate (35%-50%) to meet the needs of large-scale production
The reason is that the structure of the plasma membrane of the sperm is changed by the shock of the sperm, which seriously affects the integrity of the plasma membrane and the viability and fertilization ability of the recovered sperm.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Diluent of frozen semen of milk goat, and preparation method and diluting method thereof as well as preparation method of frozen semen of milk goat in thin pipe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0166] Step 1: Prepare base dilution:

[0167] (1) Prepare the components according to the following proportions:

[0168] Tris-aminomethane 300 mM;

[0169] Citric acid 100 mM;

[0170] Glucose 35mM;

[0171] Trehalose 260 mM;

[0172] Vitamin C 4 g / L;

[0173] Reduced glutathione 4 mM;

[0174] Bovine serum albumin 8 g / L;

[0175] Adenosine triphosphate 0.7 mM;

[0176] (2) Mix the above components with double-distilled water in proportion, filter through a 0.2 um filter membrane, and add compound antibiotics, that is, add 0.05 g / L tylosin, 0.27 g / L gentamicin, 0.16 g / L of lincomycin and 0.3 g / L of spectinomycin;

[0177] (3) According to the ratio of 15% by volume, add egg yolk additive solution to the mixed solution obtained in step 2, and mix well to obtain the basic dilution solution;

[0178] The egg yolk supplement is prepared by the following steps:

[0179] Fresh eggs free of pathogenic bacteria were soaked in chlorhexidine to extract yolk liquid, and sodiu...

Embodiment 2

[0189] Step 1: Prepare base dilution:

[0190] (1) Prepare the components according to the following proportions:

[0191] Tris-aminomethane 350 mM;

[0192] Citric acid 115 mM;

[0193] Glucose 40mM;

[0194] Trehalose 280 mM;

[0195] Vitamin C 5 g / L;

[0196] Reduced glutathione 5 mM;

[0197] Bovine serum albumin 10 g / L;

[0198] Adenosine triphosphate 1.1 mM;

[0199] (2) Mix the above components with double-distilled water in proportion, filter through a 0.2 um filter membrane, and add compound antibiotics, that is, add 0.30 g / L tylosin, 0.27 g / L gentamicin, 0.17 g / L of lincomycin and 0.30 g / L of spectinomycin;

[0200] (3) According to the ratio of 20% by volume, add egg yolk additive solution to the mixed solution obtained in step 2, and mix well to obtain the basic dilution solution;

[0201] The egg yolk supplement is prepared by the following steps:

[0202] Fresh eggs free of pathogenic bacteria were soaked in chlorhexidine to extract yolk liquid, and sodiu...

Embodiment 3

[0212] Step 1: Prepare base dilution:

[0213] (1) Prepare the components according to the following proportions:

[0214] Tris-aminomethane 400 mM;

[0215] Citric acid 130 mM;

[0216] Glucose 45mM;

[0217] Trehalose 300 mM;

[0218] Vitamin C 6 g / L;

[0219] Reduced glutathione 6 mM;

[0220] Bovine serum albumin 12 g / L;

[0221] Adenosine triphosphate 1.5 mM;

[0222] (2) Mix the above components with double-distilled water in proportion, filter through a 0.2 um filter membrane, and add compound antibiotics, that is, add 0.055 g / L tylosin, 0.28 g / L gentamicin, 0.18 g / L of lincomycin and 0.35 g / L of spectinomycin;

[0223] (3) According to the ratio of 25% by volume, add egg yolk additive solution to the mixed solution obtained in step 2, and mix well to obtain the basic dilution solution;

[0224] The egg yolk supplement is prepared by the following steps:

[0225] Fresh eggs free of pathogenic bacteria were soaked in chlorhexidine to extract yolk liquid, and so...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a diluent of frozen semen of a milk goat, and a preparation method and a diluting method thereof as well as a preparation method of frozen semen of the milk goat in a thin pipe. In an existing frozen semen storage method, sperms are struck by low temperature to cause structure change of plasmalemma, so that the integrity of the plasmalemma and the survival rate and the fertility of the sperms after resuscitation are seriously influenced. The preparation method is characterized in that thin-pipe freezing treatment with two-step dilution and balancing and two-step cooling is carried out on fresh semen, wherein a basic diluent is prepared from Tris, citric acid, glucose, trehalose, Vc, GSH, BSA, ATP, compound antibiotic, yolk and water; a frozen diluent is prepared from the basic diluent and glycerinum; the diluent is utilized for diluting and balancing the fresh semen, and the fresh semen is sub-packaged in thin pipes and is stored in liquid nitrogen. According to the invention, the effects of low-temperature storage and freezing storage of the frozen semen of the milk goat is good, the survival rate, the integration rate of acrosome and the integrity of the plasmalemma of the semen after unfreezing are effectively increased, the aberration rate is reduced and the production efficiency and the quality of the frozen semen of the milk goat are improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a dairy goat frozen semen diluent, a preparation method and a dilution method thereof, and a preparation method of dairy goat frozen semen thin tube frozen semen. Background technique [0002] Dairy goats, as an important dairy animal resource, have received much attention in recent years with the improvement of the living standard of our country and the adjustment of diet structure. Dairy goat breeding is mainly concentrated in Shaanxi, Shandong, Henan, Hebei, Yunnan and other provinces. However, the demand for goat milk has spread to more than 10 provinces, cities, autonomous regions and municipalities in the eastern coastal areas and southern Hong Kong, Macao and Taiwan, reflecting a certain Therefore, how to speed up the improvement of dairy goat breeds, improve the breeding speed of dairy goat fine breeds and establish a large-scale breeding management system has become the top...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 王维罗军史怀平席利萌孙爽高庆华张伟杨地坤朱江江石恒波
Owner NORTHWEST A & F UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap