Diluent of frozen semen of milk goat, and preparation method and diluting method thereof as well as preparation method of frozen semen of milk goat in thin pipe

Abstract

The invention relates to a diluent of frozen semen of a milk goat, and a preparation method and a diluting method thereof as well as a preparation method of frozen semen of the milk goat in a thin pipe. In an existing frozen semen storage method, sperms are struck by low temperature to cause structure change of plasmalemma, so that the integrity of the plasmalemma and the survival rate and the fertility of the sperms after resuscitation are seriously influenced. The preparation method is characterized in that thin-pipe freezing treatment with two-step dilution and balancing and two-step cooling is carried out on fresh semen, wherein a basic diluent is prepared from Tris, citric acid, glucose, trehalose, Vc, GSH, BSA, ATP, compound antibiotic, yolk and water; a frozen diluent is prepared from the basic diluent and glycerinum; the diluent is utilized for diluting and balancing the fresh semen, and the fresh semen is sub-packaged in thin pipes and is stored in liquid nitrogen. According to the invention, the effects of low-temperature storage and freezing storage of the frozen semen of the milk goat is good, the survival rate, the integration rate of acrosome and the integrity of the plasmalemma of the semen after unfreezing are effectively increased, the aberration rate is reduced and the production efficiency and the quality of the frozen semen of the milk goat are improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a dairy goat frozen semen diluent, a preparation method and a dilution method thereof, and a preparation method of dairy goat frozen semen thin tube frozen semen. Background technique [0002] Dairy goats, as an important dairy animal resource, have received much attention in recent years with the improvement of the living standard of our country and the adjustment of diet structure. Dairy goat breeding is mainly concentrated in Shaanxi, Shandong, Henan, Hebei, Yunnan and other provinces. However, the demand for goat milk has spread to more than 10 provinces, cities, autonomous regions and municipalities in the eastern coastal areas and southern Hong Kong, Macao and Taiwan, reflecting a certain Therefore, how to speed up the improvement of dairy goat breeds, improve the breeding speed of dairy goat fine breeds and establish a large-scale breeding management system has become the top...

AI Technical Summary

Problems solved by technology

The application of frozen semen technology and artificial insemination technology in dairy cows and beef cattle in my country has been relatively well developed, which has fully improved the economic benefits of breeding. However, although goat semen cryopreservation and artificial insemination technology have been used for many years, the frozen semen is generally frozen after thawing. , the sperm motility rate (0.3-0.5) is difficult to increase the conception rate (35%-50%) to meet the needs of large-scale production

The reason is that the structure of the plasma membrane of the sperm is changed by the shock of the sperm, which seriously affects the integrity of the plasma membrane and the viability and fertilization ability of the recovered sperm.

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