Sheep fresh semen preserve diluent and application thereof

A diluent, sheep technology, applied in the application, preservation of human or animal body, animal husbandry, etc., can solve the problems of short preservation time of sheep semen, destruction of plasma membrane integrity, decreased energy and vitality, etc., to prolong the effective preservation time. , improve the utilization rate, reduce the effect of feeding

Inactive Publication Date: 2015-05-20
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of the existing sheep semen preservation method that the sheep semen preservation time is short, the stored energy and vitality are reduced, the integrity of the plasma membrane is destroyed, the fertilization rate is low and the embryo mortality rate is high, and a method is provided Breeding Sheep Fresh Semen Preservation Diluent and Its Application

Method used

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  • Sheep fresh semen preserve diluent and application thereof
  • Sheep fresh semen preserve diluent and application thereof
  • Sheep fresh semen preserve diluent and application thereof

Examples

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Effect test

specific Embodiment approach 1

[0027] Specific embodiment 1: In this embodiment, a fresh sheep semen preservation diluent is prepared from the base liquid, egg yolk and astaxanthin; the volume ratio of the base liquid to the egg yolk in the sheep fresh semen preservation diluent is 8:2 ; The concentration of astaxanthin in the sheep fresh essence preservation diluent is 0.5 μmol / L~4 μmol / L.

[0028] The advantage of this implementation mode:

[0029] 1. The astaxanthin used in this embodiment is a highly efficient antioxidant, which is a carotenoid commonly found in fish, shrimp, and seaweed animals and plants; astaxanthin can effectively scavenge oxygen free radicals; Its antioxidant capacity is ten times that of mucin, lutein, canthaxanthin, carotene and other antioxidants, and one hundred times that of vitamin E; in this embodiment, astaxanthin is added to the sheep fresh essence preservation diluent , can improve the preservation effect of semen and prolong the storage time of semen;

[0030] 2. This ...

specific Embodiment approach 2

[0036] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the described base liquid is prepared according to the following method: 3.2g glucose, 3.2g sodium citrate, penicillin 100,000 IU and streptomycin 10 Ten thousand IU was dissolved in 100mL ultrapure water to obtain the base solution. Others are the same as the first embodiment.

specific Embodiment approach 3

[0037] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the concentration of astaxanthin in the sheep fresh sperm storage diluent is 0.5 μmol / L˜1 μmol / L. Others are the same as those in Embodiment 1 or 2.

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Abstract

The invention relates to a livestock semen diluent and the application thereof, and in particular to a sheep fresh semen preserve diluent and the application thereof, aiming at solving the problems that the sheep semen preservation time is short, the vigor of the preserved semen is reduced, the integrity of plasma membrane is destroyed, the fertility rate is low, and the embryo mortality is high in the existing sheep semen preservation method. The sheep fresh semen preserve diluent is prepared from basic liquid, yolk and astaxanthin, wherein the volume ratio of the basic liquid to the yolk in the sheep fresh semen preserve diluent is equal to 8 to 2, and the concentration of the astaxanthin in the sheep fresh semen preserve diluent is 0.5-4 micromol / L. The application method of the sheep fresh semen preserve diluent in sheep fresh semen preservation comprises the steps of 1. preparing the sheep fresh semen preserve diluent; 2. diluting sheep semen; and 3. preserving the semen. The sheep fresh semen preserve diluent can be obtained.

Description

technical field [0001] The invention relates to a livestock semen diluent and its application. Background technique [0002] Artificial insemination is a powerful means for efficient multiplication of elite sheep populations. The quality of semen determines the success or failure of artificial insemination technology; efficient semen preservation technology plays an important role in breed breeding and selection. [0003] At present, due to the immature freezing technology of sheep semen and the special structure of the reproductive system, most domestic sheep farms use the method of liquid storage at 4°C to store semen for a short time, and the effective storage time of semen is only 1 to 2 days. With the prolongation of storage time, liquid storage will lead to reduced sperm motility, damage to the integrity of the plasma membrane, and shortened survival time in the female reproductive tract, thereby reducing the fertilization rate and increasing the mortality of embryos. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 房义钟荣珍周道玮
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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