31 results about "Canthaxanthin" patented technology

Disclosed in the present invention is a genetically-engineered Yarrowia lipolytica strain highly producing carotenoids, which is obtained by constructing a xylose-induced activation system in the genetically-engineered Yarrowia lipolytica strain XK17 to control the induced expression of critical enzymes for synthesis of astaxanthin, wherein the critical enzymes for synthesis of astaxanthin comprise β-carotene hydroxylase CrtZ and β-carotene ketonase CrtW. Further disclosed in the present invention is the use of the genetically-engineered Yarrowia lipolytica strain in fermentation production of carotenoids. The present invention starts from the Yarrowia lipolytica strain XK17 highly producing β-carotene, and uses the xylose-induced activation system to separately control the expressions of the β-carotene hydroxylase (CrtZ) and β-carotene ketonase (CrtW) or only control the expression of the CrtW or the CrtZ, thereby separately achieving highly producing astaxanthin, or canthaxanthin, or zeaxanthin under the functions of a proper xylose induction concentration and induction opportunity.

The invention provides recombinant Escherichia coli for synthesizing carotene pigments. The recombinant Escherichia coli comprises the following multiple modifications: (a) introducing and expressing an mvaS gene, an mvaE gene and an mvk gene; (b) introducing and expressing a pmk gene and an mvd gene; (c) introducing and expressing a crtB gene, a crtI gene, a crtE gene and an idi gene; the recombinant Escherichia coli capable of producing canthaxanthin, beta-carotene or astaxanthin at high yield is prepared, the yield of by-products is reduced while the yield of target objects is increased, the bottleneck problem in industrial production is solved, and the recombinant Escherichia coli can be used for mass production of canthaxanthin, beta-carotene or astaxanthin industry.

The invention relates to the technical field of biological medicines, in particular to application of canthaxanthin extract and a feed additive containing the canthaxanthin extract in silkworm breeding, and finds that cocoon shell amount, whole cocoon amount and pupa weight of silkworms can be remarkably increased and egg laying amount of single moths can be increased by adding canthaxanthin extract to silkworms for the first time. Meanwhile, the silkworm variety with functional carotenoid binding protein can also be induced to produce orange red natural colored cocoons. The invention further provides a compound feed additive containing the canthaxanthin extract and the sericin-derived small peptide and / or gamma-aminobutyric acid. Experiments prove that the canthaxanthin extract and the sericin-derived small peptide have a synergistic effect in the aspect of increasing the cocoon shell amount. According to the technical scheme, on the premise that normal growth and development of silkworms are not affected, the multiple purposes of yield increasing, propagation promoting and color increasing are achieved at the same time, and the method has good application prospects and industrial value.

The invention discloses a method for promoting yarrowia lipolytica to synthesize carotenoid, which comprises the following steps of: recombining and expressing beta-carotene hydroxylase CrtZ and / or beta-carotene ketolase CrtW fused with a short peptide label in the yarrowia lipolytica producing beta-carotene; site-specific mutagenesis is carried out on the amino acid site of CrtZ; and a CrtZ coding gene is integrated in a multi-copy manner. The invention also discloses a yarrowia lipolytica genetically engineered bacterium for producing beta-carotene, wherein carotenoid is selected from zeaxanthin, canthaxanthin and astaxanthin. The yield of zeaxanthin, canthaxanthin and astaxanthin in yarrowia lipolytica is remarkably increased through enzyme label engineering, combinatorial optimization and site-specific mutagenesis strategies, and a new path is provided for efficient synthesis of carotenoids.

The application provides a preparation method of an oxygen-containing carotenoid, in particular a preparation method of canthaxanthin (I-a) or astaxanthin (I-b), and the preparation method comprises the following steps: (1) C15-alcohol (II) is reacted with 2-mercaptobenzothiazole (MBT) to prepare C15-benzothiazole sulfide (III), and the C15-benzothiazole sulfide (III) is further oxidized to prepare C15-benzothiazole sulfone (IV); (2) the C15-benzothiazole sulfone (IV) is coupled with C10-dialdehyde (V) to prepare the target product: canthaxanthin (I-a) or astaxanthin (I-b); the method has low raw material cost, avoids the use of phosphorus salt in the traditional synthesis route, and the by-product 2-hydroxybenzothiazole is an important intermediate for synthesizing the herbicide thiobencarb, and also has economic benefits; and the method provides an efficient, economical and environmentally-friendly solution for large-scale clean production of canthaxanthin and astaxanthin.

The present invention provides a tetraazacyclododecane tetraacetic acid metal catalyst that can be used to oxidize β-carotene to canthaxanthin using hydrogen peroxide as an oxidant. This process provides mild conditions, high product yield, and high selectivity for the all-trans isomer. Furthermore, the catalyst has the advantages of low toxicity, minimal environmental pollution, and recyclability.

The present invention relates to the use of vitamin D3, 25-OH-D3 and / or 25-OH-D3 and canthaxanthin to improve fertility in birds, preferably poultry, more preferably laying hens. The invention also relates to a method for improving the fertility of birds. The method comprises a feeding regimen and a feed adaptive to the age of the birds.

The present invention relates to the use of Vit-D3, 25-OH-D3 and / or 25-OH-D3 and Canthaxanthin improves fertility in birds, preferably poultry, more preferably layers. The present invention further relates to a method to improve fertility in birds comprising a feeding regime and a feed adapted to the age of the bird.

The invention relates to the technical field of food characterization recognition and authentic identification, in particular to a characteristic difference metabolite composition of carthamus tinctorius from different producing areas and a screening method and application of the characteristic difference metabolite composition. The characteristic difference metabolite composition is selected from the group consisting of L-phenylalanine, L-citrulline, L-arginine, L-tyrosine, L-asparagine, L-aspartic acid, rutin, quercetin, quercitrin, kaempferol, baicalein, eriodictyol, isoquercitrin, kaempferol-3-O-glucoside, dihydrokaempferol, canthaxanthin, epicatechin, tryptamine and the like; the surfactant is selected from two or more of fumaric acid and betaine. The characteristic difference metabolite composition is obtained by screening through a screening method based on a metabonomics analysis technology, can be used for tracing the origin of safflower, is high in tracing accuracy and simple and convenient to operate, and has good economic benefits and social values.

The invention provides application of canthaxanthin in preparation of a medicine for treating and / or relieving xerophthalmia, and relates to the technical field of medicine. According to the application, the distribution condition of an eye tissue structure drug target TMEM16A ion channel is researched, a TMEM16A conditional knockout mouse is constructed through a gene knockout means, and through research on the treatment effect of the knockout mouse, it is verified that canthaxanthin can serve as an activator of the TMEM16A ion channel, and the canthaxanthin can be used as an activator of the TMEM16A ion channel. Therefore, the purpose of application in preparation of the medicine for treating and / or relieving xerophthalmia is achieved.

The invention discloses a strain of Yarrowia lipolytica YL_Xqs36 capable of producing high amounts of astaxanthin, canthaxanthin and beta-carotene. The strain is deposited in China Center for Type Culture Collection, with a deposit date of February 26, 2024 and a deposit number of CCTCC NO: M 2024333. The Yarrowia lipolytica YL_Xqs36 can produce carotenoid products such as astaxanthin, canthaxanthin and beta-carotene simultaneously, and the total pigment content of the dry thalli reaches 36 mg / g (UV detection). The dry thalli detected by liquid chromatography has an astaxanthin content of 15 mg / g, a beta-carotene content of 4.2 mg / g and a canthaxanthin content of 2.4 mg / g. The dry thalli of the strain is particularly suitable for the development of feed for aquatic animals such as shrimps, crabs, salmon, large yellow croaker, grouper and ornamental fish.

The present invention relates to a novel Schizochytrium sp. strain containing a high protein content, antioxidant pigments and omega-3 fatty acids, and a method for producing a biomass containing a high protein content, antioxidant pigments and omega-3 fatty acids by using the strain. The novel Schizochytrium sp. strain of the present invention simultaneously contains, in the cells thereof, a high content of protein ingredients including amino acids such as glutamic acid, arginine and aspartic acid, antioxidant pigments of beta-carotene and canthaxanthin, and omega-3 unsaturated fatty acid ingredients including eicosapentaenoic acid and docosahexaenoic acid, and thus the strain itself or the biomass and bio-oil produced by culturing and fermenting the strain can be effectively used as a feed composition or a food composition and the like.

To provide a technique relating to treatment of nausea and / or vomiting.SOLUTION: Administering to a subject in need thereof a therapeutically effective amount of a C50 carotenoids compound. The C50 carotenoids compound is selected from the group consisting of decaprenoxanthin, C50 - astaxanthin, C50 - beta-carotene, C50 - carotene (n = 3) (1616 - diisopentenylphytoene), C50 - zeaxanthin, C50 - caroxanthin, C50 - nostoxanthin, sarsinaxanthin, sarprenoxanthin, acyclic C50 carotenoids bacterioruberin, C50 - canthaxanthin, - lycopene, - phytoene, and combinations thereof. C50 C50.SELECTED DRAWING: Figure 1A

The application discloses a preparation method and application of canthaxanthin, and comprises the following steps: in the presence of beta-carotene, a catalyst, an additive and a solvent, the canthaxanthin is prepared by reaction at a temperature of-20 DEG C to 20 DEG C for 5-24 hours; the catalyst comprises a mixture of chlorate, metal iodide and inorganic acid; the additive comprises a mixture of raw materials represented by formula I, formula II and formula III, wherein the mass ratio of the raw materials represented by formula I, formula II and formula III is 1:(1.0-5.0):(1.0-5.0); and the total additive amount of the additive is 0.01-1 times the mass of the beta-carotene. The preparation method of the canthaxanthin has the characteristics of fast reaction speed, high product yield, less generation of three wastes, strong coloring capacity of the product and excellent thermal stability, and has great competitive advantage in the field of food and drug additives.

This invention discloses a high-yield canthaxanthin genetically engineered bacterium, its construction method, and its applications, relating to the fields of microbial metabolic engineering and synthetic biology. The construction method includes the following steps: introducing key genes into a yeast chassis strain for overexpression to obtain a basic canthaxanthin-producing bacterium; the key genes include... carB Gene, carRP Gene, crBKT Gene, HMG1 Genes and GGS1 Genes; through repeated gene introduction optimization, the quality of the cantharidin-producing strain was improved. crBKT The copy number of the gene was determined to obtain a copy number-optimized strain; the copy number-optimized strain was then... ERG7 The high-yield canthaxanthin genetically engineered bacterium was obtained by performing site-directed mutagenesis to shrink the substrate binding pocket of the gene. The high-yield canthaxanthin genetically engineered bacterium provided by this invention can significantly improve the synthesis efficiency and production yield of canthaxanthin, and has great application prospects in the field of canthaxanthin biosynthesis.

The invention belongs to the field of microbial technology and medicine, and particularly relates to an adhesive engineering yeast probiotic preparation as well as a preparation method and application thereof. The engineering saccharomycetes for efficiently expressing cantharides yellow is constructed by applying a synthetic biological technology. On the basis, the adhesion engineering yeast probiotics MBPSc22 are prepared through noradrenaline auto-polymerization, and the survival rate and retention capacity of the engineering yeast in gastrointestinal tracts are improved while the macrophage targeting capacity of the engineering yeast is not damaged. The adhesive engineering probiotic preparation can express canthaxanthin, directionally remove active oxygen at an inflammation part, reduce the inflammation level, increase the abundance and diversity of intestinal microorganisms, optimize the structure of the intestinal microorganisms and recover the steady state of the intestinal microorganisms, so that the aim of treating colitis is fulfilled.

To provide an oil-soluble pigment emulsified preparation which uses alkenylsuccinic acid esterified starch having high emulsion stability and color tone stability during storage.SOLUTION: An oil-soluble pigment preparation is an oil-in-water type emulsified composition including an aqueous phase containing alkenylsuccinic acid esterified starch and a water-soluble polysaccharide, and an oil phase containing an oil-soluble pigment, where a pH of the aqueous phase is 3.5 or lower. The oil-soluble pigment desirably includes xanthophylls such as astaxanthin, capsanthin, canthaxanthin, cryptoxanthin, zeaxanthin, fucoxanthin, lutein, capsicum pigment (alternatively: paprika pigment), marigold pigment, orange pigment, phaffia pigment, and haematococcus algae pigment.SELECTED DRAWING: None

The invention discloses a preparation method of a high-trans high-content canthaxanthin crystal, and belongs to the field of fine chemical engineering. The invention relates to a preparation method of a high-trans high-content canthaxanthin crystal. The preparation method comprises the following steps: adding crude canthaxanthin into an isomerization crystallization solvent A and an auxiliary agent B, and heating for isomerization; and cooling, crystallizing, centrifuging, and carrying out vacuum drying on a solid-phase material to obtain a canthaxanthin crystal. By combining isomerization and crystallization into one-step treatment, the total yield is higher, the raw materials are simple and easy to obtain, less three wastes are generated, and the method is environment-friendly.

The invention discloses a recombinant beta-carotene ketolase as well as a mutant and application thereof, and belongs to the technical field of biology. According to the invention, the recombinant beta-carotene ketolase of which the catalytic activity is obviously improved is obtained by carrying out structural domain recombination on the amino acid sequences from the 1st site to the 134 site in the beta-carotene ketolase HpCrtW from haematococcus pluvialis and the amino acid sequences from the 149th site to the 328th site in the beta-carotene ketolase trCrCrtW from chlamydomonas reinhardtii; furthermore, the recombinant beta-carotene ketolase is modified through semi-rational design, so that the obtained recombinant beta-carotene ketolase mutant has relatively good catalytic activity, and the yield of canthaxanthin can be remarkably increased; furthermore, the constructed recombinant genetic engineering strain containing the recombinant beta-carotene ketolase or the mutant of the recombinant beta-carotene ketolase can produce canthaxanthin with high yield by taking glucose as a substrate, and has a remarkable industrial application value.

The present invention relates to a Chlamydomonas reinhardtii mutant strain having an improved ability to produce keto-carotenoid pigments, particularly astaxanthin and canthaxanthin. When used, the strain of the present invention exhibits excellent lipid production ability compared to conventional strains, and can produce keto-carotenoid pigments, specifically astaxanthin and canthaxanthin, and thus can efficiently produce pigments on an industrial scale. In addition, the strain can be applied as a raw material for foods, health functional foods and medicines comprising pigments.

The present invention relates to a new process for the production of new specific intermediates, which are preferably used in the production of vitamin A, vitamin A acetate, or β-carotene and derivatives thereof, e.g. canthaxanthin, astaxanthin or zeaxanthin.

The application discloses a Cantharellus cibarius polysaccharide, a preparation method and application thereof. The Cantharellus cibarius polysaccharide is obtained by hot water extraction, ethanol precipitation, protein removal, ion exchange column chromatography and dialysis of Cantharellus cibarius fruiting bodies. The Cantharellus cibarius polysaccharide is a heteropolysaccharide composed of beta-D-glucose and alpha-D-galactose, and the molar ratio of residues of beta-D-glucose and alpha-D-galactose is 1:19. The Cantharellus cibarius polysaccharide prepared by the application has significant immunoregulation and anti-tumor activity, and can be applied in medicines, health products or food.

The present application belongs to the field of microbial technology and medicine, and particularly relates to an adhesive engineered yeast probiotic preparation and a preparation method and application thereof. The present application uses synthetic biology technology to construct an engineered yeast strain capable of efficiently expressing canthaxanthin. On this basis, an adhesive engineered yeast probiotic MBP_Sc22 is prepared through norepinephrine self-polymerization, which improves the survival rate and retention capacity of the engineered yeast in the gastrointestinal tract without impairing the ability of the engineered yeast to target macrophages. The adhesive engineered yeast probiotic preparation expresses canthaxanthin, removes active oxygen at the inflammation site, down-regulates the inflammation level, increases the intestinal microbial abundance and diversity, optimizes the intestinal microbial structure and restores the intestinal microbial homeostasis, thereby achieving the purpose of treating colitis.

The present invention relates to the use of Vit-D3, 25-OH-D3 and / or 25-OH-D3 and Canthaxanthin improves fertility in birds, preferably poultry, more preferably layers. The present invention further relates to a method to improve fertility in birds comprising a feeding regime and a feed adapted to the age of the bird.

The invention belongs to the technical field of food detection, and discloses a method for simultaneously detecting various carotenoids in egg yolk. According to the method, the C30 reversed-phase chromatographic column is creatively adopted, and an optimized binary mobile phase gradient elution procedure is combined, so that the problem of co-elution of structural analogues (especially lutein and zeaxanthin) is successfully solved. The method provided by the invention realizes simultaneous baseline separation of five main carotenoids, namely lutein, zeaxanthin, cantharidin yellow, beta-cryptoxanthin and beta-carotene, peak shapes are sharp and symmetrical, the separation degree is obviously superior to that of a traditional C18 chromatographic column method, and a solid foundation is laid for accurate quantification.

The invention discloses a yarrowia lipolytica engineering bacterium for producing carotenoids at high yield, which is obtained by constructing a xylose induced activation system in the yarrowia lipolytica engineering bacterium XK17 to control the induced expression of astaxanthin synthesis key enzyme, the key enzymes for synthesizing the astaxanthin comprise beta-carotene hydroxylase CrtZ and beta-carotene ketolase CrtW. The invention also discloses an application of the yarrowia lipolytica engineering bacterium in fermentation production of carotenoids. According to the method, starting from yarrowia lipolytica XK17 with high yield of beta-carotene, a xylose induction activation system is used for respectively controlling the expression of beta-carotene hydroxylase (CrtZ) and beta-carotene ketolase (CrtW), or only controlling the expression of CrtW or CrtZ; the high yield of astaxanthin or canthaxanthin or zeaxanthin can be realized under the action of proper xylose induction concentration and induction opportunity respectively.

The invention relates to the field of microbial engineering and genetic breeding, and discloses a Chovulochroma zoffii mutant strain capable of producing astaxanthin and canthaxanthin and an application of the Chovulochroma zoffii mutant strain, including a Chovulochroma zoffii mutant strain named as Cz-R1 and an application of the Chovulochroma zoffii mutant strain in production of astaxanthin and canthaxanthin, and particularly relates to a Chovulochroma zoffii mutant strain named as Cz-R1 and an application of the Chovulochroma zoffii mutant strain in production of astaxanthin and canthaxanthin. The mutant strain of the Zhovuchroma viridis retains the growth attribute of a wild strain of the Zhovuchroma viridis under a glucose heterotrophic condition, has the capability of high-density fermentation, and can improve the yield and accumulation amount of astaxanthin and canthaxanthin.

The present invention relates to a solid formulation comprising xanthophylls, such as astaxanthin or canthaxanthin with a high amount of a specific stereochemical form. Furthermore, the present invention relates to a process of production of such a specific formulation.

The invention belongs to the technical field of fine chemical engineering, and particularly relates to a preparation method of canthaxanthin. The preparation method comprises the following steps: adding a catalyst and beta-carotene into a reaction solvent, adjusting the pH value of the solution to be acidic, pre-cooling the reaction solution, circularly injecting the reaction solution into a chromatographic column filled with oxidant particles through a peristaltic pump, returning to a reaction system, controlling the temperature of the circulating solution, washing with water after the reaction is finished, and drying to obtain beta-carotene. And after standing and layering, carrying out reduced pressure distillation on the obtained organic phase to separate the solvent, carrying out isomerization reaction, filtering, and recrystallizing to obtain canthaxanthin. According to the preparation method disclosed by the invention, the cis-isomer content is low in the reaction process, the isomerization reaction time is short, the efficiency is high, and the obtained cantharidin product is high in quality, less in residual inorganic salt and less in washing wastewater, and is more environment-friendly.