17 results about "Canthaxanthin" patented technology

The invention relates to the technical field of biological medicines, in particular to application of canthaxanthin extract and a feed additive containing the canthaxanthin extract in silkworm breeding, and finds that cocoon shell amount, whole cocoon amount and pupa weight of silkworms can be remarkably increased and egg laying amount of single moths can be increased by adding canthaxanthin extract to silkworms for the first time. Meanwhile, the silkworm variety with functional carotenoid binding protein can also be induced to produce orange red natural colored cocoons. The invention further provides a compound feed additive containing the canthaxanthin extract and the sericin-derived small peptide and / or gamma-aminobutyric acid. Experiments prove that the canthaxanthin extract and the sericin-derived small peptide have a synergistic effect in the aspect of increasing the cocoon shell amount. According to the technical scheme, on the premise that normal growth and development of silkworms are not affected, the multiple purposes of yield increasing, propagation promoting and color increasing are achieved at the same time, and the method has good application prospects and industrial value.

The invention discloses a method for promoting yarrowia lipolytica to synthesize carotenoid, which comprises the following steps of: recombining and expressing beta-carotene hydroxylase CrtZ and / or beta-carotene ketolase CrtW fused with a short peptide label in the yarrowia lipolytica producing beta-carotene; site-specific mutagenesis is carried out on the amino acid site of CrtZ; and a CrtZ coding gene is integrated in a multi-copy manner. The invention also discloses a yarrowia lipolytica genetically engineered bacterium for producing beta-carotene, wherein carotenoid is selected from zeaxanthin, canthaxanthin and astaxanthin. The yield of zeaxanthin, canthaxanthin and astaxanthin in yarrowia lipolytica is remarkably increased through enzyme label engineering, combinatorial optimization and site-specific mutagenesis strategies, and a new path is provided for efficient synthesis of carotenoids.

The application provides a preparation method of an oxygen-containing carotenoid, in particular a preparation method of canthaxanthin (I-a) or astaxanthin (I-b), and the preparation method comprises the following steps: (1) C15-alcohol (II) is reacted with 2-mercaptobenzothiazole (MBT) to prepare C15-benzothiazole sulfide (III), and the C15-benzothiazole sulfide (III) is further oxidized to prepare C15-benzothiazole sulfone (IV); (2) the C15-benzothiazole sulfone (IV) is coupled with C10-dialdehyde (V) to prepare the target product: canthaxanthin (I-a) or astaxanthin (I-b); the method has low raw material cost, avoids the use of phosphorus salt in the traditional synthesis route, and the by-product 2-hydroxybenzothiazole is an important intermediate for synthesizing the herbicide thiobencarb, and also has economic benefits; and the method provides an efficient, economical and environmentally-friendly solution for large-scale clean production of canthaxanthin and astaxanthin.

The present invention relates to the use of vitamin D3, 25-OH-D3 and / or 25-OH-D3 and canthaxanthin to improve fertility in birds, preferably poultry, more preferably laying hens. The invention also relates to a method for improving the fertility of birds. The method comprises a feeding regimen and a feed adaptive to the age of the birds.

The invention provides application of canthaxanthin in preparation of a medicine for treating and / or relieving xerophthalmia, and relates to the technical field of medicine. According to the application, the distribution condition of an eye tissue structure drug target TMEM16A ion channel is researched, a TMEM16A conditional knockout mouse is constructed through a gene knockout means, and through research on the treatment effect of the knockout mouse, it is verified that canthaxanthin can serve as an activator of the TMEM16A ion channel, and the canthaxanthin can be used as an activator of the TMEM16A ion channel. Therefore, the purpose of application in preparation of the medicine for treating and / or relieving xerophthalmia is achieved.

To provide a technique relating to treatment of nausea and / or vomiting.SOLUTION: Administering to a subject in need thereof a therapeutically effective amount of a C50 carotenoids compound. The C50 carotenoids compound is selected from the group consisting of decaprenoxanthin, C50 - astaxanthin, C50 - beta-carotene, C50 - carotene (n = 3) (1616 - diisopentenylphytoene), C50 - zeaxanthin, C50 - caroxanthin, C50 - nostoxanthin, sarsinaxanthin, sarprenoxanthin, acyclic C50 carotenoids bacterioruberin, C50 - canthaxanthin, - lycopene, - phytoene, and combinations thereof. C50 C50.SELECTED DRAWING: Figure 1A

The application discloses a preparation method and application of canthaxanthin, and comprises the following steps: in the presence of beta-carotene, a catalyst, an additive and a solvent, the canthaxanthin is prepared by reaction at a temperature of-20 DEG C to 20 DEG C for 5-24 hours; the catalyst comprises a mixture of chlorate, metal iodide and inorganic acid; the additive comprises a mixture of raw materials represented by formula I, formula II and formula III, wherein the mass ratio of the raw materials represented by formula I, formula II and formula III is 1:(1.0-5.0):(1.0-5.0); and the total additive amount of the additive is 0.01-1 times the mass of the beta-carotene. The preparation method of the canthaxanthin has the characteristics of fast reaction speed, high product yield, less generation of three wastes, strong coloring capacity of the product and excellent thermal stability, and has great competitive advantage in the field of food and drug additives.

This invention discloses a high-yield canthaxanthin genetically engineered bacterium, its construction method, and its applications, relating to the fields of microbial metabolic engineering and synthetic biology. The construction method includes the following steps: introducing key genes into a yeast chassis strain for overexpression to obtain a basic canthaxanthin-producing bacterium; the key genes include... carB Gene, carRP Gene, crBKT Gene, HMG1 Genes and GGS1 Genes; through repeated gene introduction optimization, the quality of the cantharidin-producing strain was improved. crBKT The copy number of the gene was determined to obtain a copy number-optimized strain; the copy number-optimized strain was then... ERG7 The high-yield canthaxanthin genetically engineered bacterium was obtained by performing site-directed mutagenesis to shrink the substrate binding pocket of the gene. The high-yield canthaxanthin genetically engineered bacterium provided by this invention can significantly improve the synthesis efficiency and production yield of canthaxanthin, and has great application prospects in the field of canthaxanthin biosynthesis.

The invention belongs to the field of microbial technology and medicine, and particularly relates to an adhesive engineering yeast probiotic preparation as well as a preparation method and application thereof. The engineering saccharomycetes for efficiently expressing cantharides yellow is constructed by applying a synthetic biological technology. On the basis, the adhesion engineering yeast probiotics MBPSc22 are prepared through noradrenaline auto-polymerization, and the survival rate and retention capacity of the engineering yeast in gastrointestinal tracts are improved while the macrophage targeting capacity of the engineering yeast is not damaged. The adhesive engineering probiotic preparation can express canthaxanthin, directionally remove active oxygen at an inflammation part, reduce the inflammation level, increase the abundance and diversity of intestinal microorganisms, optimize the structure of the intestinal microorganisms and recover the steady state of the intestinal microorganisms, so that the aim of treating colitis is fulfilled.

The invention discloses a recombinant beta-carotene ketolase as well as a mutant and application thereof, and belongs to the technical field of biology. According to the invention, the recombinant beta-carotene ketolase of which the catalytic activity is obviously improved is obtained by carrying out structural domain recombination on the amino acid sequences from the 1st site to the 134 site in the beta-carotene ketolase HpCrtW from haematococcus pluvialis and the amino acid sequences from the 149th site to the 328th site in the beta-carotene ketolase trCrCrtW from chlamydomonas reinhardtii; furthermore, the recombinant beta-carotene ketolase is modified through semi-rational design, so that the obtained recombinant beta-carotene ketolase mutant has relatively good catalytic activity, and the yield of canthaxanthin can be remarkably increased; furthermore, the constructed recombinant genetic engineering strain containing the recombinant beta-carotene ketolase or the mutant of the recombinant beta-carotene ketolase can produce canthaxanthin with high yield by taking glucose as a substrate, and has a remarkable industrial application value.

The present invention relates to a new process for the production of new specific intermediates, which are preferably used in the production of vitamin A, vitamin A acetate, or β-carotene and derivatives thereof, e.g. canthaxanthin, astaxanthin or zeaxanthin.

The application discloses a Cantharellus cibarius polysaccharide, a preparation method and application thereof. The Cantharellus cibarius polysaccharide is obtained by hot water extraction, ethanol precipitation, protein removal, ion exchange column chromatography and dialysis of Cantharellus cibarius fruiting bodies. The Cantharellus cibarius polysaccharide is a heteropolysaccharide composed of beta-D-glucose and alpha-D-galactose, and the molar ratio of residues of beta-D-glucose and alpha-D-galactose is 1:19. The Cantharellus cibarius polysaccharide prepared by the application has significant immunoregulation and anti-tumor activity, and can be applied in medicines, health products or food.

The present application belongs to the field of microbial technology and medicine, and particularly relates to an adhesive engineered yeast probiotic preparation and a preparation method and application thereof. The present application uses synthetic biology technology to construct an engineered yeast strain capable of efficiently expressing canthaxanthin. On this basis, an adhesive engineered yeast probiotic MBP_Sc22 is prepared through norepinephrine self-polymerization, which improves the survival rate and retention capacity of the engineered yeast in the gastrointestinal tract without impairing the ability of the engineered yeast to target macrophages. The adhesive engineered yeast probiotic preparation expresses canthaxanthin, removes active oxygen at the inflammation site, down-regulates the inflammation level, increases the intestinal microbial abundance and diversity, optimizes the intestinal microbial structure and restores the intestinal microbial homeostasis, thereby achieving the purpose of treating colitis.

The invention belongs to the technical field of food detection, and discloses a method for simultaneously detecting various carotenoids in egg yolk. According to the method, the C30 reversed-phase chromatographic column is creatively adopted, and an optimized binary mobile phase gradient elution procedure is combined, so that the problem of co-elution of structural analogues (especially lutein and zeaxanthin) is successfully solved. The method provided by the invention realizes simultaneous baseline separation of five main carotenoids, namely lutein, zeaxanthin, cantharidin yellow, beta-cryptoxanthin and beta-carotene, peak shapes are sharp and symmetrical, the separation degree is obviously superior to that of a traditional C18 chromatographic column method, and a solid foundation is laid for accurate quantification.

The invention relates to the field of microbial engineering and genetic breeding, and discloses a Chovulochroma zoffii mutant strain capable of producing astaxanthin and canthaxanthin and an application of the Chovulochroma zoffii mutant strain, including a Chovulochroma zoffii mutant strain named as Cz-R1 and an application of the Chovulochroma zoffii mutant strain in production of astaxanthin and canthaxanthin, and particularly relates to a Chovulochroma zoffii mutant strain named as Cz-R1 and an application of the Chovulochroma zoffii mutant strain in production of astaxanthin and canthaxanthin. The mutant strain of the Zhovuchroma viridis retains the growth attribute of a wild strain of the Zhovuchroma viridis under a glucose heterotrophic condition, has the capability of high-density fermentation, and can improve the yield and accumulation amount of astaxanthin and canthaxanthin.

The invention belongs to the field of analytical chemistry, and particularly relates to a method for simultaneously detecting various carotenoids. The invention provides a method for detecting carotenoid in a tomato sample by utilizing an HPLC (High Performance Liquid Chromatography) method, which comprises the following steps: carrying out isocratic elution by adopting an Inertsil ODS-3 chromatographic column and taking methanol, acetonitrile and dichloromethane in a volume ratio of 7: 10: 3 as a mobile phase formula, and detecting the sample in a detector; the carotenoid comprises at least one of astaxanthin, xanthophyll, cantharidin, zeaxanthin or beta-carotene. According to the method, astaxanthin, zeaxanthin, beta-carotene and lycopene in a sample can be effectively eluted, separated and detected, main peaks can be completely separated, and the analysis method is high in specificity, has a better linear curve in a low concentration range, is not influenced by personnel and instruments, and is stable and reliable.