3 results about "Canthaxanthin" patented technology

The application provides a preparation method of an oxygen-containing carotenoid, in particular a preparation method of canthaxanthin (I-a) or astaxanthin (I-b), and the preparation method comprises the following steps: (1) C15-alcohol (II) is reacted with 2-mercaptobenzothiazole (MBT) to prepare C15-benzothiazole sulfide (III), and the C15-benzothiazole sulfide (III) is further oxidized to prepare C15-benzothiazole sulfone (IV); (2) the C15-benzothiazole sulfone (IV) is coupled with C10-dialdehyde (V) to prepare the target product: canthaxanthin (I-a) or astaxanthin (I-b); the method has low raw material cost, avoids the use of phosphorus salt in the traditional synthesis route, and the by-product 2-hydroxybenzothiazole is an important intermediate for synthesizing the herbicide thiobencarb, and also has economic benefits; and the method provides an efficient, economical and environmentally-friendly solution for large-scale clean production of canthaxanthin and astaxanthin.

This invention discloses a high-yield canthaxanthin genetically engineered bacterium, its construction method, and its applications, relating to the fields of microbial metabolic engineering and synthetic biology. The construction method includes the following steps: introducing key genes into a yeast chassis strain for overexpression to obtain a basic canthaxanthin-producing bacterium; the key genes include... carB Gene, carRP Gene, crBKT Gene, HMG1 Genes and GGS1 Genes; through repeated gene introduction optimization, the quality of the cantharidin-producing strain was improved. crBKT The copy number of the gene was determined to obtain a copy number-optimized strain; the copy number-optimized strain was then... ERG7 The high-yield canthaxanthin genetically engineered bacterium was obtained by performing site-directed mutagenesis to shrink the substrate binding pocket of the gene. The high-yield canthaxanthin genetically engineered bacterium provided by this invention can significantly improve the synthesis efficiency and production yield of canthaxanthin, and has great application prospects in the field of canthaxanthin biosynthesis.