99 results about "Canthaxanthin" patented technology

The invention provides a method for preparing canthaxanthin. The method comprises that: in water and organic solvent immiscible with the water, under the action of hydrogen peroxide catalyst, alkali metal chlorate or bromate and beta-carotene are subjected to oxidation reaction, wherein the oxidation reaction is carried out by adding hydrogen peroxide into a mixture containing the beta-carotene, the water, metal iodide, the organic solvent immiscible with the water and the alkali metal chlorate or the alkali metal bromate to obtain reaction mixed solution. The method can efficiently produce the canthaxanthin in an industrialized mode in short time.

The invention relates to the technical field of aquaculture feedstuff, in particular to feedstuff additive premix material. The feedstuff additive comprises the following components according to the following weight portion: 150 to 450 portions of astaxanthin, 300 to 700 portions of dunaliella sallina, 150 to 400 portions of vitamin E, 300 to 600 portions of Chinese thorowax, 100 to 250 portions of canthaxanthin, 300 to 600 portions of antioxidant, and 150 to 1,000 portions of corn protein powder. The feedstuff additive has no innocuous and side residue, no drug resistance, environmental protection, and no adverse effect on the feedstuff palatability. The feedstuff additive can obviously improve the body color of fishes in the yellow and red color systems such as golden pompano, pseudobagrus fulvidraco, clarias fuscus, sea bream, large yellow croaker, and the like, can make the body color display natural yellow or red color, and can effectively improve the immunity, reproductive capacity and meat quality of cultivated fish. In addition, the feedstuff additive has stable raw material sources and a simple manufacturing process.

An object of the present invention is to provide a method for microbiologically producing astaxanthin of high concentration at low cost while suppressing production of canthaxanthin. Specifically, the present invention relates to a method for producing carotenoids including astaxanthin comprising culturing a bacterium that concurrently produces astaxanthin and canthaxanthin in a medium containing biotin, wherein a ratio of concentration of produced canthaxanthin to concentration of produced astaxanthin in a culture product after the end of culture in the medium is lower than that in a culture product after the end of culture in a biotin-free medium.

Provided is a method for expressing an introduced gene or genes in a C1 metabolizing microorganism host wherein the gene(s) are integrated into the tig region of the chromosome. This method provides high level expression in a stable manner in which growth rate of the host strain is not highly affected and a selection marker is not required. The use of this method for expressing carotenoid biosynthetic genes and resulting production of canthaxanthin is also described.

The invention relates to a feed premix for yellow hair chickens. The feed premix is characterized by comprising the following raw materials in part by weight: 10-15 parts of composite vitamins, 100-120 parts of composite mineral substances, 125-150 parts of salt, 45-60 parts of choline chloride, 40-70 parts of prebiotics, 20-25 parts of coccidium-resistance drugs, 2-6 parts of complex enzyme, 25-60 parts of emulgator, 100-180 parts of lysine, 80-130 parts of methionine hydroxy analog-Ca, 10-20 parts of enramycin, 7-15 parts of antioxidants, 80-120 parts of lutein, 4-8 parts of canthaxanthin, and 50-350 parts of wheat middling. The feed premix has the advantages that the coloring of the yellow hair chickens is stable, and particularly in high-temperature summer, the good coloring of carcass can be ensured, and the coloring of feet diameters can reach more than 10 degrees of a Roche yolk color fan; a selected colorant mainly comprises the natural lutein extracted from marigold flowers, and according to the technology provided by the invention the application amount of the lutein and the canthaxanthin can be greatly reduced, thus playing a great role in protection of food safety; and the cost of pigments can be reduced greatly, and the competitiveness of yellow hair chicken production enterprises can be improved.

Novel CrtW carotenoid ketolase are provided that are useful for the production of ketocarotenoids. The ketolases genes of the present invention exhibit low homology in comparison to other CrtW ketolases previously reported. Expression of the carotenoid ketolases in heterologous hosts enabled production of canthaxanthin and astaxanthin. Coexpression experiments using divergent crtW genes resulted in increased production of the desired ketocarotenoids.

The present disclosure describes a method for the continuous dissolution of e.g. crystalline astaxanthin or canthaxanthin suspension in oil, by applying heat for a predetermined period using an in-line or optionally batch wise-process, followed by cooling the heated solution with an oil phase or heat exchanger. The resulting oil solution may be used as such or added directly to feed components which are turned into feed compositions by extrusion, compaction or granulation. Alternatively the oil solution is spray coated or directly loaded to extruded or compacted pellets and granulates. The disclosure avoids using formulated carotenoids prepared by known multi-step production methods requiring solvents, solvent removal, cooling and emulsification with an aqueous phase or precipitation and recovery steps. Thus the present invention is a more economical, efficient and environmentally friendly method to add nutrients and colourants to feed compositions instead of using colloid dispersed particulate hydrocolloid compositions.

The invention discloses a novel synthesis method for preparing canthaxanthin by beta-carotene one-step oxidization. According to the method, beta-carotene serves as an initial raw material, a cyclodextrin compound serves as a phase transfer catalyst, molecular oxygen serves as an oxidizing agent under catalysis of a copper compound and amino acid, and the canthaxanthin is prepared by one-step oxidization. The route is high in reaction selectivity and simple in process and facilitates industrial production.

Discloses the formation of carotenoid compounds containing 4-keto-β-ionene rings such as astaxanthin or canthaxanthin in higher plant flowers, and particularly in the corolla and reproductive parts of higher plant flowers, wherein said Petals of higher plants produce carotenoid compounds containing β-ionene rings such as β-carotene or zeaxanthin, but otherwise do not produce astaxanthin or canthaxanthin. One or more genes regulated by a promoter are inserted (transformed) into higher plants. The inserted gene encodes a chimeric enzyme comprising (a) a carotenoid-forming enzyme that is at least a ketolase. The gene is operably linked to (b) a plastid-directed transit peptide. Some of the higher plants to be transformed produce at least zeaxanthin or beta-carotene in their pre-transformation flowers, while other plants produce only minimal, if any, colored carotenoid pigments prior to transformation, and use carotenoid Gene forming cassettes for transformation. Transformation methods and uses of transformed plants are described.

The invention provides a method for preparing canthaxanthin by oxidizing beta-carotene. The method comprises the following steps of: dissolving beta-carotene in a solvent, and carrying out oxidation reaction in the presence of a catalyst and an oxidant to prepare canthaxanthin; wherein the catalyst is a metal calcium salt compound. The cheap metal calcium salt compound is used as the catalyst, anda peroxide is used as the oxidant to catalyze the oxidation reaction, so that the method has the advantages of mild process route conditions, environment friendliness, simple and convenient operationand easy industrial production.

The invention discloses a method for determining the residual amount of a canthaxanthin pigment in eggs. The method comprises the following steps: (1) weighing an egg sample to be determined, adding pyrogallic acid, adding an acetonitrile extracting solution, carrying out vortex mixing and ultrasonic extraction in sequence, and transferring the supernatant; fixing the volume with acetonitrile after combining the supernatant extracted in two times, and mixing uniformly; drying a part of the supernatant under nitrogen, dissolving the residue with the acetonitrile, and centrifuging and filteringto obtain a sample solution; (2) analyzing the sample solution on a liquid chromatograph to determine the canthaxanthin peak area; and (3) determining the canthaxanthin peak area with multiple groupsof canthaxanthin solutions with known mass concentration, performing linear regression by taking the canthaxanthin mass concentration as a horizontal axis and taking the corresponding peak area as a longitudinal axis, and establishing a canthaxanthin linear regression equation; and calculating the residual amount of the canthaxanthin pigment on the egg to be determined according to the canthaxanthin peak area of the egg sample to be determined. The method has the characteristics of easiness, fastness, accuracy and sensitivity, and is suitable for the detection and analysis of the residual amount of canthaxanthin pigment in eggs.

The invention discloses a specific monoclonal antibody capable of identifying beta-carotene pigments and an enzyme-linked immunosorbent assay (ELISA) method and kit for detecting beta-carotene pigments. The monoclonal antibody is secreted by a hybridoma cell DCC/C11 and the hybridoma cell is collected in the China Center for Type Culture Collection, with collection number being CCTCC NO:C201146. The ELISA method comprises the steps of preparation of immunogen, coating antigen and the antibody, treatment and detection of samples, and the like. The ELISA method and the kit can detect the total content of canthaxanthin, beta-carotene, beta-apo-8'-carotenal, xanthophyll, capsorubin and beta-ionone in the samples by one step, thus shortening the detection time and lowering the detection cost; and the ELISA method and the kit have the characteristics of high detection sensitivity, good precision and good accuracy.

The invention relates to a method for detecting canthaxanthin residues in animals, and belongs to the technical field of food safety detection. The detection method of the present invention is an improvement on the basis of the prior art, and its improvement points are: a. preparation of canthaxanthin standard solution: take a certain amount of canthaxanthin standard product and place it in a 100ml brown volumetric flask , add N,N-dimethylformamide to make the volume to 100ml, shake well, and store it as a stock solution in a -4°C refrigerator; weigh a certain amount of the stock solution, and prepare standard solutions of different concentrations with methanol solution : 0.05μg/ml, 0.1μg/ml, 0.2μg/ml, 0.5μg/ml, 1.0μg/ml, 2.0μg/ml, 5.0μg/ml, 10.0μg/ml and 20.0μg/ml; b. Chromatographic conditions : Waters2695 high performance liquid chromatograph attaches ultraviolet detector, chromatographic column Symmetry C18 5 μ m, 3.9 * 150mm, wavelength 475nm, flow velocity 1.0ml/min, mobile phase V (acetonitrile): V (water)=95: 5, column temperature 30 ℃, the injection volume is 50 μl. The invention has the advantages of being simple, fast, accurate and reliable, and is suitable for the analysis and detection of large batches of samples.

The present invention discloses a feed for relieving combined poisoning of cadmium and lead for laying hens. The feed comprises the following components: corn, soybean meal, soybean oil, calcium carbonate, calcium hydrophosphate, edible salt, choline chloride, compound multi-vitamins, mineral premixes, DL-methionine, probiotics, an organic acidifying agent and premixes. The premixes comprise the following components: tea polyphenols, natural vitamin E, vitamin A, vitamin C, yeast selenium, canthaxanthin, 25-hydroxyl-D3 and rice chaffs. A preparation method is as follows: the corn and soybean meal are crushed to a particle size of 1,500 [mu]m, and then each of the components is mixed evenly in a step-by-step method to prepare the compound feed. The feed solves the problems of reduced production performances and deteriorated eggshell quality due to the combined poisoning of the cadmium and lead for the laying hens, can improve the anti-oxidation ability of the commercial laying hen organisms, and alleviates the occurrences of the poisoning of the cadmium and lead for the laying hens.

The invention belongs to the field of organic synthesis, and specifically discloses a method for preparing canthaxanthin. The method comprises the following steps: dissolving beta-carotene, an oxidantI and a phase transfer catalyst in an organic solvent; adding an oxidant II at 0-15 DEG C; performing oxidation reaction at 0-15 DEG C after the addition is completed; and then, separating an oil phase from the obtained oxidation reaction product and performing isomerization reaction to obtain all-trans canthaxanthin, wherein the oxidant I is a peroxide oxidant, and the oxidant II is a hypochlorite oxidant. The method for preparing canthaxanthin provided by the invention can be adopted to significantly increase the yield of reaction, reduce the dosage of the oxidants and shorten the reactiontime.

The invention discloses a method for simultaneously determining seven pigments in eggs, and belongs to the technical field of food safety detection. According to the method, a DQTP/Fe3O4 organic covalent skeleton structure and a magnetic nano composite material are adopted as a distributed magnetic solid-phase extraction adsorbent to extract pigments in eggs, and then an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method is combined with an external standard method to determine the content of the pigments in the eggs. The invention discloses a method forsimultaneously determining contents of riboflavin, xanthophyll, canthaxanthin, Sudan red I, Sudan red II, Sudan red III and Sudan red IV pigments in eggs. Detection limits of xanthophyll, canthaxanthin, Sudan red I, Sudan red II, Sudan red III and Sudan red IV are 0.2 mu g/kg; the detection limit of riboflavin is 0.2 mu g/kg. The adding amount of the seven pigments is within the range of 2-10 mu g/kg, the recovery rate is 80.2-106.7%, and relative standard deviation RSD is 1.5-6.0%.

The invention relates to an astaxanthin fertilizer containing microbes and a preparation method thereof. The astaxanthin fertilizer is prepared a fertilizer body and microbes and is characterized by also containing agricultural astaxanthin and canthaxanthin, wherein the agricultural astaxanthin is prepared from astaxanthin, chitin and chitosan. The agricultural astaxanthin is obtained by fermenting crab shells and/or shrimp shells through microbes, and the microbes are one or more lactic acid bacteria and saccharomycetes according to a certain ratio. The astaxanthin fertilizer can improve thestress resistance of plants and the quality of crops.