99 results about "Canthaxanthin" patented technology

Astaxanthin is a potent antioxidant, over 500 times more powerful than Vitamin E and 10 times stronger than other carotenoids such as zeaxanthin, lutein, canthaxanthin and beta-carotene. Astaxanthin has also been shown to enhance and modulate the immune system and diminish the damaging effects of UVA sunlight. Disclosed is a method for retarding and ameliorating fever blisters (cold sores) and canker sores. The method comprises administering a source of astaxanthin in a therapeutically effective amount to prevent, retard and ameliorate fever blisters and canker sores. The astaxanthin may be administered orally, topically, or in a combination of oral and topical dosage.

The invention provides a method for preparing canthaxanthin. The method comprises that: in water and organic solvent immiscible with the water, under the action of hydrogen peroxide catalyst, alkali metal chlorate or bromate and beta-carotene are subjected to oxidation reaction, wherein the oxidation reaction is carried out by adding hydrogen peroxide into a mixture containing the beta-carotene, the water, metal iodide, the organic solvent immiscible with the water and the alkali metal chlorate or the alkali metal bromate to obtain reaction mixed solution. The method can efficiently produce the canthaxanthin in an industrialized mode in short time.

Methanotrophic bacterial strains are provided that have been optimized for the production of carotenoid compounds through the down-regulation of one or more of the crtN1, aid, crtN2 and crtN3 genes of the carotenoid biosynthetic pathway. The resulting strains lack pigmented C30 carotenoid compounds, and show an increase in the production of C40 carotenoids. The use of the optimized host strains for the production of the C40 carotenoids canthaxanthin and astaxanthin is also described.

The invention relates to a body color intensifier for large yellow croakers. The body color intensifier is prepared from the following components in percentage by weight: 20-45wt% of astaxanthin, 20-45wt% of lutein and 20-45wt% of canthaxanthin. The body color intensifier disclosed by the invention is used as a feed additive used in the artificial compound feed for the large yellow croakers. The additive amount is only 0.01-0.15%, and the body color of the artificially cultured large yellow croakers can be obviously improved after the body color intensifier is used about 8 weeks. Meanwhile, the body color intensifier disclosed by the invention is used together with a large yellow croaker feed prepared by the applicant to obtain a preferable effect.

The invention relates to the technical field of aquaculture feedstuff, in particular to feedstuff additive premix material. The feedstuff additive comprises the following components according to the following weight portion: 150 to 450 portions of astaxanthin, 300 to 700 portions of dunaliella sallina, 150 to 400 portions of vitamin E, 300 to 600 portions of Chinese thorowax, 100 to 250 portions of canthaxanthin, 300 to 600 portions of antioxidant, and 150 to 1,000 portions of corn protein powder. The feedstuff additive has no innocuous and side residue, no drug resistance, environmental protection, and no adverse effect on the feedstuff palatability. The feedstuff additive can obviously improve the body color of fishes in the yellow and red color systems such as golden pompano, pseudobagrus fulvidraco, clarias fuscus, sea bream, large yellow croaker, and the like, can make the body color display natural yellow or red color, and can effectively improve the immunity, reproductive capacity and meat quality of cultivated fish. In addition, the feedstuff additive has stable raw material sources and a simple manufacturing process.

An object of the present invention is to provide a method for microbiologically producing astaxanthin of high concentration at low cost while suppressing production of canthaxanthin. Specifically, the present invention relates to a method for producing carotenoids including astaxanthin comprising culturing a bacterium that concurrently produces astaxanthin and canthaxanthin in a medium containing biotin, wherein a ratio of concentration of produced canthaxanthin to concentration of produced astaxanthin in a culture product after the end of culture in the medium is lower than that in a culture product after the end of culture in a biotin-free medium.

The invention discloses a method for preparing canthaxanthin through oxidation.The method includes the steps that beta-carotene serves as the raw material, sulfur oxide urea or urea peroxide serves as an oxidizing agent, an oxidizing reaction is conducted, and the canthaxanthin is obtained.The method has the advantages that operation is simple, the reaction is stable, and the trigger condition is mild; the oxidizing agents including sulfur oxide urea and urea peroxide can be decomposed into H2O2 and elemental oxygen in solvent, O2 is released slowly, the content of active oxygen is high, stability is high, the utility of time is long, no side effect exists, no toxin or public hazard exists, and it is beneficial to achieve industrialized clean production.

The invention discloses a method for preparing canthaxanthin by utilizing oxidized beta-carotene. The method for preparing canthaxanthin comprises the following steps of: dissolving beta-carotene shown in a formula (I) (described in the specification) into an organic solvent, dropwise adding an oxidizing agent, namely a sodium periodate (NaIO4) aqueous solution, and adding a catalyst, namely sodium iodide (NaI), then stirring and reacting to obtain canthaxanthin shown in a formula (II). The organic solvent is one or combination of more than one of the following components: halogenated hydrocarbon containing 1-6 carbon atoms and fatty acid ester containing 3-10 carbon atoms. The method for preparing canthaxanthin has the advantages of mild reaction conditions, simple operation, low cost and environmental friendliness and is applicable to industrial production.

Provided is a method for expressing an introduced gene or genes in a C1 metabolizing microorganism host wherein the gene(s) are integrated into the tig region of the chromosome. This method provides high level expression in a stable manner in which growth rate of the host strain is not highly affected and a selection marker is not required. The use of this method for expressing carotenoid biosynthetic genes and resulting production of canthaxanthin is also described.

The invention discloses a method for preparing canthaxanthin by electrocatalytic oxygen-oxidized beta-carotene. The raw material beta-carotene is dissolved in an organic solvent, a gas oxygen source is used as an oxidizing agent, and electrolytic reaction is carried out in an electrocatalytic oxidation device filled with catalysts for one-step synthesis to obtain the canthaxanthin. The method forpreparing the canthaxanthin by the electrocatalytic oxygen-oxidized beta-carotene has the advantages that the reaction condition is mild, the reaction process is easy to control, green and environmental protection are achieved, the method for preparing the canthaxanthin by the electrocatalytic oxygen-oxidized beta-carotene is suitable for industrial production, and the yield of the canthaxanthin is higher than 95%.

It is intended to provide a process for conveniently and efficiently producing large amounts of astaxanthin and canthaxanthin from a material which can be easily obtained. A microorganism Thraustochytrium sp. CHN-3 (FERM P-18556) capable of producing astaxanthin and canthaxanthin, which belongs to the genus Labyrinthula and the Thraustochytrium species, is cultured and thus astaxanthin and canthaxanthin are accumulated in the microbial cells. Then the cells are separated from the medium and astaxanthin and canthaxanthin are collected from the separated cells by extracting with a solvent.

The invention relates to a feed premix for yellow hair chickens. The feed premix is characterized by comprising the following raw materials in part by weight: 10-15 parts of composite vitamins, 100-120 parts of composite mineral substances, 125-150 parts of salt, 45-60 parts of choline chloride, 40-70 parts of prebiotics, 20-25 parts of coccidium-resistance drugs, 2-6 parts of complex enzyme, 25-60 parts of emulgator, 100-180 parts of lysine, 80-130 parts of methionine hydroxy analog-Ca, 10-20 parts of enramycin, 7-15 parts of antioxidants, 80-120 parts of lutein, 4-8 parts of canthaxanthin, and 50-350 parts of wheat middling. The feed premix has the advantages that the coloring of the yellow hair chickens is stable, and particularly in high-temperature summer, the good coloring of carcass can be ensured, and the coloring of feet diameters can reach more than 10 degrees of a Roche yolk color fan; a selected colorant mainly comprises the natural lutein extracted from marigold flowers, and according to the technology provided by the invention the application amount of the lutein and the canthaxanthin can be greatly reduced, thus playing a great role in protection of food safety; and the cost of pigments can be reduced greatly, and the competitiveness of yellow hair chicken production enterprises can be improved.

The present invention relates to C15 phosphonate compound for synthesizing canthaxanthin and its preparation process. The C15 phosphonate compound is made to react with 2, 7-dimethyl-2, 4, 6-octyltriene-1, 8-dialdehyde under the action of alkali to obtain 4, 4'-dihydroxy-beta-carotene; and 4, 4'-dihydroxy-beta-carotene is then oxidized with manganese dioxideor aluminum isopropoxide to obtain canthaxanthin. The new synthesis path is superior to available industrial canthaxanthin synthesizing process, and has simple technological process, high yield, low cost and no use of triphenyl phosphine and chlorinated dialkyl phosphite.

Novel CrtW carotenoid ketolase are provided that are useful for the production of ketocarotenoids. The ketolases genes of the present invention exhibit low homology in comparison to other CrtW ketolases previously reported. Expression of the carotenoid ketolases in heterologous hosts enabled production of canthaxanthin and astaxanthin. Coexpression experiments using divergent crtW genes resulted in increased production of the desired ketocarotenoids.

The invention discloses a method for preparing canthaxanthin through oxidation of beta-carotene. The method comprises the steps as follows: beta-carotene is dissolved in an organic solvent and oxidized to canthaxanthin with an oxidizing agent capable of producing oxygen atoms under the action of an allyl oxidation catalyst shown in a compound I and a cocatalyst capable of producing iodine anions.The method has the benefits as follows: halate waste difficult to treat is not produced and the method has the advantages of being green, environmentally friendly and high in yield.

The present disclosure describes a method for the continuous dissolution of e.g. crystalline astaxanthin or canthaxanthin suspension in oil, by applying heat for a predetermined period using an in-line or optionally batch wise-process, followed by cooling the heated solution with an oil phase or heat exchanger. The resulting oil solution may be used as such or added directly to feed components which are turned into feed compositions by extrusion, compaction or granulation. Alternatively the oil solution is spray coated or directly loaded to extruded or compacted pellets and granulates. The disclosure avoids using formulated carotenoids prepared by known multi-step production methods requiring solvents, solvent removal, cooling and emulsification with an aqueous phase or precipitation and recovery steps. Thus the present invention is a more economical, efficient and environmentally friendly method to add nutrients and colourants to feed compositions instead of using colloid dispersed particulate hydrocolloid compositions.

The invention discloses a novel synthesis method for preparing canthaxanthin by beta-carotene one-step oxidization. According to the method, beta-carotene serves as an initial raw material, a cyclodextrin compound serves as a phase transfer catalyst, molecular oxygen serves as an oxidizing agent under catalysis of a copper compound and amino acid, and the canthaxanthin is prepared by one-step oxidization. The route is high in reaction selectivity and simple in process and facilitates industrial production.

Discloses the formation of carotenoid compounds containing 4-keto-β-ionene rings such as astaxanthin or canthaxanthin in higher plant flowers, and particularly in the corolla and reproductive parts of higher plant flowers, wherein said Petals of higher plants produce carotenoid compounds containing β-ionene rings such as β-carotene or zeaxanthin, but otherwise do not produce astaxanthin or canthaxanthin. One or more genes regulated by a promoter are inserted (transformed) into higher plants. The inserted gene encodes a chimeric enzyme comprising (a) a carotenoid-forming enzyme that is at least a ketolase. The gene is operably linked to (b) a plastid-directed transit peptide. Some of the higher plants to be transformed produce at least zeaxanthin or beta-carotene in their pre-transformation flowers, while other plants produce only minimal, if any, colored carotenoid pigments prior to transformation, and use carotenoid Gene forming cassettes for transformation. Transformation methods and uses of transformed plants are described.

The invention provides a method for preparing canthaxanthin by oxidizing beta-carotene. The method comprises the following steps of: dissolving beta-carotene in a solvent, and carrying out oxidation reaction in the presence of a catalyst and an oxidant to prepare canthaxanthin; wherein the catalyst is a metal calcium salt compound. The cheap metal calcium salt compound is used as the catalyst, anda peroxide is used as the oxidant to catalyze the oxidation reaction, so that the method has the advantages of mild process route conditions, environment friendliness, simple and convenient operationand easy industrial production.

The invention discloses a method for determining the residual amount of a canthaxanthin pigment in eggs. The method comprises the following steps: (1) weighing an egg sample to be determined, adding pyrogallic acid, adding an acetonitrile extracting solution, carrying out vortex mixing and ultrasonic extraction in sequence, and transferring the supernatant; fixing the volume with acetonitrile after combining the supernatant extracted in two times, and mixing uniformly; drying a part of the supernatant under nitrogen, dissolving the residue with the acetonitrile, and centrifuging and filteringto obtain a sample solution; (2) analyzing the sample solution on a liquid chromatograph to determine the canthaxanthin peak area; and (3) determining the canthaxanthin peak area with multiple groupsof canthaxanthin solutions with known mass concentration, performing linear regression by taking the canthaxanthin mass concentration as a horizontal axis and taking the corresponding peak area as a longitudinal axis, and establishing a canthaxanthin linear regression equation; and calculating the residual amount of the canthaxanthin pigment on the egg to be determined according to the canthaxanthin peak area of the egg sample to be determined. The method has the characteristics of easiness, fastness, accuracy and sensitivity, and is suitable for the detection and analysis of the residual amount of canthaxanthin pigment in eggs.

The invention discloses a specific monoclonal antibody capable of identifying beta-carotene pigments and an enzyme-linked immunosorbent assay (ELISA) method and kit for detecting beta-carotene pigments. The monoclonal antibody is secreted by a hybridoma cell DCC / C11 and the hybridoma cell is collected in the China Center for Type Culture Collection, with collection number being CCTCC NO:C201146. The ELISA method comprises the steps of preparation of immunogen, coating antigen and the antibody, treatment and detection of samples, and the like. The ELISA method and the kit can detect the total content of canthaxanthin, beta-carotene, beta-apo-8'-carotenal, xanthophyll, capsorubin and beta-ionone in the samples by one step, thus shortening the detection time and lowering the detection cost; and the ELISA method and the kit have the characteristics of high detection sensitivity, good precision and good accuracy.

The invention relates to a method for detecting canthaxanthin residues in animals, and belongs to the technical field of food safety detection. The detection method of the present invention is an improvement on the basis of the prior art, and its improvement points are: a. preparation of canthaxanthin standard solution: take a certain amount of canthaxanthin standard product and place it in a 100ml brown volumetric flask , add N,N-dimethylformamide to make the volume to 100ml, shake well, and store it as a stock solution in a -4°C refrigerator; weigh a certain amount of the stock solution, and prepare standard solutions of different concentrations with methanol solution : 0.05μg / ml, 0.1μg / ml, 0.2μg / ml, 0.5μg / ml, 1.0μg / ml, 2.0μg / ml, 5.0μg / ml, 10.0μg / ml and 20.0μg / ml; b. Chromatographic conditions : Waters2695 high performance liquid chromatograph attaches ultraviolet detector, chromatographic column Symmetry C18 5 μ m, 3.9 * 150mm, wavelength 475nm, flow velocity 1.0ml / min, mobile phase V (acetonitrile): V (water)=95: 5, column temperature 30 ℃, the injection volume is 50 μl. The invention has the advantages of being simple, fast, accurate and reliable, and is suitable for the analysis and detection of large batches of samples.

The formation of a carotenoid compound containing a 4-keto-β-ionene ring such as astaxanthin or canthaxanthin in flowers, and particularly in the corolla and reproductive parts of a flower of a higher plant whose flowers produce a carotenoid compound containing a β-ionene ring such as β-carotene or zeaxanthin, but otherwise do not produce astaxanthin or canthaxanthin is disclosed. One or more genes controlled by a promoter are inserted (transformed) into a higher plant. The inserted gene encodes a chimeric enzyme including (a) a carotenoid-forming enzyme that is at least a ketolase. That gene is operatively linked to (b) a plastid-directed transit peptide. Some higher plants to be transformed produce at least zeaxanthin or β-carotene in their flowers prior to transformation, whereas other plants produce little if any colored carotenoid pigments prior to transformation and are transformed with a cassette of carotenoids-forming genes. Methods of transformation and use of the transformed plants are described.

The present invention discloses a feed for relieving combined poisoning of cadmium and lead for laying hens. The feed comprises the following components: corn, soybean meal, soybean oil, calcium carbonate, calcium hydrophosphate, edible salt, choline chloride, compound multi-vitamins, mineral premixes, DL-methionine, probiotics, an organic acidifying agent and premixes. The premixes comprise the following components: tea polyphenols, natural vitamin E, vitamin A, vitamin C, yeast selenium, canthaxanthin, 25-hydroxyl-D3 and rice chaffs. A preparation method is as follows: the corn and soybean meal are crushed to a particle size of 1,500 [mu]m, and then each of the components is mixed evenly in a step-by-step method to prepare the compound feed. The feed solves the problems of reduced production performances and deteriorated eggshell quality due to the combined poisoning of the cadmium and lead for the laying hens, can improve the anti-oxidation ability of the commercial laying hen organisms, and alleviates the occurrences of the poisoning of the cadmium and lead for the laying hens.

The invention belongs to the field of organic synthesis, and specifically discloses a method for preparing canthaxanthin. The method comprises the following steps: dissolving beta-carotene, an oxidantI and a phase transfer catalyst in an organic solvent; adding an oxidant II at 0-15 DEG C; performing oxidation reaction at 0-15 DEG C after the addition is completed; and then, separating an oil phase from the obtained oxidation reaction product and performing isomerization reaction to obtain all-trans canthaxanthin, wherein the oxidant I is a peroxide oxidant, and the oxidant II is a hypochlorite oxidant. The method for preparing canthaxanthin provided by the invention can be adopted to significantly increase the yield of reaction, reduce the dosage of the oxidants and shorten the reactiontime.

The invention discloses a method for simultaneously determining seven pigments in eggs, and belongs to the technical field of food safety detection. According to the method, a DQTP / Fe3O4 organic covalent skeleton structure and a magnetic nano composite material are adopted as a distributed magnetic solid-phase extraction adsorbent to extract pigments in eggs, and then an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) method is combined with an external standard method to determine the content of the pigments in the eggs. The invention discloses a method forsimultaneously determining contents of riboflavin, xanthophyll, canthaxanthin, Sudan red I, Sudan red II, Sudan red III and Sudan red IV pigments in eggs. Detection limits of xanthophyll, canthaxanthin, Sudan red I, Sudan red II, Sudan red III and Sudan red IV are 0.2 mu g / kg; the detection limit of riboflavin is 0.2 mu g / kg. The adding amount of the seven pigments is within the range of 2-10 mu g / kg, the recovery rate is 80.2-106.7%, and relative standard deviation RSD is 1.5-6.0%.

The invention relates to a culture method, in particular to a method for culturing bloody parrots, which is suitable for producing bloody parrots with yellow and orange body colors. The technology comprises the following steps of: culturing the bloody parrots under normal conditions; and converting the body colors of the bloody parrots into yellow, orange and the like by adopting the technology. In the technology, according to the characteristic of pigment metabolism of the bloody parrots, lutein, canthaxanthin and the like are added into a feed and the bloody parrots different from the common red parrots are cultured; and the bloody parrots have special colors such as yellow, orange and the like to meet market demand.

The invention discloses a catalyst for preparing canthaxanthin from beta-carotene and a preparation method and application thereof. The catalyst is a phosphorus-doped graphite-phase carbon nitride loaded metalloporphyrin complex, and has the advantages that the catalytic activity is high, the combination between active components and a carrier is firmer, the active components are not easy to lose, the stability is good, and the like. The method for preparing canthaxanthin by carrying out oxidation reaction on beta-carotene and hydrogen peroxide in the presence of the catalyst, a cocatalyst and a solvent has the advantages of mild reaction process, high all-trans product selectivity, high product yield and small environmental pollution, and the catalyst can be recycled.

The invention relates to an astaxanthin fertilizer containing microbes and a preparation method thereof. The astaxanthin fertilizer is prepared a fertilizer body and microbes and is characterized by also containing agricultural astaxanthin and canthaxanthin, wherein the agricultural astaxanthin is prepared from astaxanthin, chitin and chitosan. The agricultural astaxanthin is obtained by fermenting crab shells and / or shrimp shells through microbes, and the microbes are one or more lactic acid bacteria and saccharomycetes according to a certain ratio. The astaxanthin fertilizer can improve thestress resistance of plants and the quality of crops.