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Method for simultaneously determining seven pigments in eggs

A technology of eggs and pigments, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of qualitative and quantitative analysis of multiple pigment substances, single target object, etc.

Active Publication Date: 2020-09-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The limitation of the above method is that the detection target is relatively single or less, and it is impossible to conduct qualitative and quantitative analysis of multiple pigment substances in egg yolk at the same time, so as to distinguish natural nutrients from artificially added pigments.

Method used

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  • Method for simultaneously determining seven pigments in eggs
  • Method for simultaneously determining seven pigments in eggs
  • Method for simultaneously determining seven pigments in eggs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Magnetic DQTP / Fe 3 o 4 Preparation methods of nanomaterials

[0022] Place a 3 mL test tube containing 17 mg of 2,6-diaminoanthraquinone (DQ) in DMF in a water bath at 90°C. 10mg of 1,3,5-trimethylphloroglucinol (TP) was formulated with DMF to make 10mg / L, and 0.05mL of 6mol / L acetic acid solution was added to the solution of acetic acid, which was fully ultrasonicated, and then 1mL was slowly added dropwise to DQ with a pipette gun. In hot solution, the process is controlled within 1h. After the TP solution was added completely, plug the test tube mouth with degreased cotton, and continue to mix the solution in a water bath at 90°C for 3 hours. The resultant was soaked in deionized water and acetone for 24 h to replace unreacted ligands, and dried in vacuum to obtain DQTP. 80mg FeCl 3 ·6H 2 O and 50 mg FeSO 4 ·7H 2 O was dispersed in 20 mL of pure water. Add 70 mg of synthesized DQTP and ultrasonically disperse at 80°C to prepare a suspension. 30% ...

Embodiment 2

[0023] Embodiment 2: A kind of pretreatment method of extracting 7 kinds of pigments in eggs

Embodiment 3

[0026] Example 3: A method for detecting the content of 7 pigments in eggs

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Abstract

The invention discloses a method for simultaneously determining seven pigments in eggs, and belongs to the technical field of food safety detection. According to the method, a DQTP / Fe3O4 organic covalent skeleton structure and a magnetic nano composite material are adopted as a distributed magnetic solid-phase extraction adsorbent to extract pigments in eggs, and then an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) method is combined with an external standard method to determine the content of the pigments in the eggs. The invention discloses a method forsimultaneously determining contents of riboflavin, xanthophyll, canthaxanthin, Sudan red I, Sudan red II, Sudan red III and Sudan red IV pigments in eggs. Detection limits of xanthophyll, canthaxanthin, Sudan red I, Sudan red II, Sudan red III and Sudan red IV are 0.2 mu g / kg; the detection limit of riboflavin is 0.2 mu g / kg. The adding amount of the seven pigments is within the range of 2-10 mu g / kg, the recovery rate is 80.2-106.7%, and relative standard deviation RSD is 1.5-6.0%.

Description

technical field [0001] The invention relates to a method for simultaneously measuring seven pigments in eggs, belonging to the technical field of food safety detection. Background technique [0002] With the continuous improvement of living standards, people's pursuit of natural and organic ingredients is becoming a trend. Eggs produced by free-range chickens under natural conditions, commonly known as "native eggs" or "grass eggs", are favored, and the price is twice or even several times higher than ordinary eggs. Ordinary consumers mainly distinguish native eggs from ordinary eggs by the color of the yolk, thinking that native eggs are darker in color. The color of egg yolk comes from pigment substances such as riboflavin and lutein. Free-range chickens have more opportunities to eat leaves and seeds rich in lutein. Lutein is a natural carotenoid, which is enriched in egg yolk after metabolism, making it appear bright orange. Cage chickens mainly eat artificial feed, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/04G01N30/72G01N30/34G01N30/86G01N30/88
CPCG01N30/02G01N30/04G01N30/06G01N30/14G01N30/34G01N30/72G01N30/8634G01N30/8679G01N30/88G01N2030/047G01N2030/062G01N2030/065G01N2030/146G01N2030/884
Inventor 杨成黄晴沈晓芳孙嵇成
Owner JIANGNAN UNIV
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