Detection method of canthaxanthin residues in animals

A detection method and canthaxanthin technology, which are applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of low recovery rate, unsatisfactory results when the amount is small, and can not meet the requirements of experiments, and achieve the effect of accurate results.

Inactive Publication Date: 2011-12-21
YUNNAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

The spectrophotometer method can only be used for the situation with a large amount of residue, and the result is not ideal for a small amount
In addition, there are studies on the determination of canthaxanthin in feed by high performanc...

Method used

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  • Detection method of canthaxanthin residues in animals
  • Detection method of canthaxanthin residues in animals
  • Detection method of canthaxanthin residues in animals

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Embodiment Construction

[0022] The method for detecting canthaxanthin residues in animals of the present invention is an improvement on the basis of the prior art, and its specific steps are as follows:

[0023] 1. Preparation of canthaxanthin standard solution

[0024] (1) Weigh a certain amount of canthaxanthin standard substance and place it in a 100ml brown volumetric flask.

[0025] (2) ①Add N,N-dimethylformamide to dilute to 100ml, shake well, and store in a -4°C refrigerator as a stock solution.

[0026] ② Add acetonitrile to make up to 100ml, shake well, and store it as a stock solution in a -4°C refrigerator.

[0027] Through observation, dissolving canthaxanthin with N, N-dimethylformamide can make it dissolve completely, and the solution is clear and transparent, and the color is darker; dissolving canthaxanthin with acetonitrile can not make it dissolve completely, there are particles, and the solution is cloudy, The color is lighter. Therefore, in this step, we choose N,N-dimethylform...

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Abstract

The invention relates to a method for detecting canthaxanthin residues in animals, and belongs to the technical field of food safety detection. The detection method of the present invention is an improvement on the basis of the prior art, and its improvement points are: a. preparation of canthaxanthin standard solution: take a certain amount of canthaxanthin standard product and place it in a 100ml brown volumetric flask , add N,N-dimethylformamide to make the volume to 100ml, shake well, and store it as a stock solution in a -4°C refrigerator; weigh a certain amount of the stock solution, and prepare standard solutions of different concentrations with methanol solution : 0.05μg/ml, 0.1μg/ml, 0.2μg/ml, 0.5μg/ml, 1.0μg/ml, 2.0μg/ml, 5.0μg/ml, 10.0μg/ml and 20.0μg/ml; b. Chromatographic conditions : Waters2695 high performance liquid chromatograph attaches ultraviolet detector, chromatographic column Symmetry C18 5 μ m, 3.9 * 150mm, wavelength 475nm, flow velocity 1.0ml/min, mobile phase V (acetonitrile): V (water)=95: 5, column temperature 30 ℃, the injection volume is 50 μl. The invention has the advantages of being simple, fast, accurate and reliable, and is suitable for the analysis and detection of large batches of samples.

Description

Technical field: [0001] The invention relates to a method for detecting canthaxanthin residues in animals, and belongs to the technical field of food safety detection. Background technique: [0002] In recent years, with the continuous improvement of people's living standards in our country, people's consumption demand for animal-derived food has increased year by year, but animal-derived food safety issues are very prominent, except for mad cow disease, foot-and-mouth disease, bird flu and other zoonotic diseases In addition to the threat of diseases, major food safety incidents such as clenbuterol, chloramphenicol, Sudan red, and melamine also occur frequently, making people pay more and more attention to the abuse of additives in food. Especially since the incident of "red duck eggs" caused by the addition of Sudan Red in food, the abuse and harm of feed colorants have attracted widespread attention. [0003] Canthaxanthin (Canthaxanthin), also known as canthaxanthin and...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 徐昆龙肖蓉田洋杨兰兰李良李栋何梦恒王丽屏
Owner YUNNAN AGRICULTURAL UNIVERSITY
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