Detection method of canthaxanthin residues in animals

Abstract

The invention relates to a method for detecting canthaxanthin residues in animals, and belongs to the technical field of food safety detection. The detection method of the present invention is an improvement on the basis of the prior art, and its improvement points are: a. preparation of canthaxanthin standard solution: take a certain amount of canthaxanthin standard product and place it in a 100ml brown volumetric flask , add N,N-dimethylformamide to make the volume to 100ml, shake well, and store it as a stock solution in a -4°C refrigerator; weigh a certain amount of the stock solution, and prepare standard solutions of different concentrations with methanol solution : 0.05μg / ml, 0.1μg / ml, 0.2μg / ml, 0.5μg / ml, 1.0μg / ml, 2.0μg / ml, 5.0μg / ml, 10.0μg / ml and 20.0μg / ml; b. Chromatographic conditions : Waters2695 high performance liquid chromatograph attaches ultraviolet detector, chromatographic column Symmetry C18 5 μ m, 3.9 * 150mm, wavelength 475nm, flow velocity 1.0ml / min, mobile phase V (acetonitrile): V (water)=95: 5, column temperature 30 ℃, the injection volume is 50 μl. The invention has the advantages of being simple, fast, accurate and reliable, and is suitable for the analysis and detection of large batches of samples.

Description

Technical field: [0001] The invention relates to a method for detecting canthaxanthin residues in animals, and belongs to the technical field of food safety detection. Background technique: [0002] In recent years, with the continuous improvement of people's living standards in our country, people's consumption demand for animal-derived food has increased year by year, but animal-derived food safety issues are very prominent, except for mad cow disease, foot-and-mouth disease, bird flu and other zoonotic diseases In addition to the threat of diseases, major food safety incidents such as clenbuterol, chloramphenicol, Sudan red, and melamine also occur frequently, making people pay more and more attention to the abuse of additives in food. Especially since the incident of "red duck eggs" caused by the addition of Sudan Red in food, the abuse and harm of feed colorants have attracted widespread attention. [0003] Canthaxanthin (Canthaxanthin), also known as canthaxanthin and...

AI Technical Summary

Problems solved by technology

The spectrophotometer method can only be used for the situation with a large amount of residue, and the result is not ideal for a small amount

In addition, there are studies on the determination of canthaxanthin in feed by high performance liquid chromatography. Canthaxanthin has two structures, formal and trans. This method can only separate one structure, and when used to extract tissues and organs, The recovery rate is low and cannot meet the requirements of the experiment

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