Detection kit and detection method of calcium peroxide in flour
A detection kit and calcium peroxide technology, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, can solve the problems that there is no unified method for calcium peroxide detection, etc. Achieve the effect of simple operation
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Embodiment 1
[0052] According to the actual requirements of the site, it is only necessary to determine whether the flour contains calcium peroxide. In the above cases, the following steps are adopted:
[0053] (1) Take an appropriate amount of flour into a sample bottle, add water and stir, and after fully dissolving, add an acid-base indicator dropwise. If the sample solution changes color after adding the indicator, it is judged that the flour must be doped with alkaline substances, and the flour is unqualified;
[0054] (2) If the solution does not change color after adding the indicator, take an appropriate amount of flour into the sample bottle again, add water to make the flour a semi-fluid viscous paste, add 1% sulfuric acid solution, and quickly add 10% sodium hydroxide solution to stir , add 3-aminophthalhydrazide solution and potassium ferricyanide reagent in turn, then place the sample bottle in a dark environment, and observe the color change;
[0055] If the sample in the sam...
Embodiment 2
[0057] According to the actual requirements on site, it is also necessary to determine the content of calcium peroxide in the flour. In the above cases, the following steps are adopted:
[0058] (1) Weigh an appropriate amount of flour into a sample bottle, add an organic solvent to fully shake, oscillate, let stand for a while, and discard the supernatant;
[0059] (2) Add 1% dilute sulfuric acid solution to the sample after discarding the supernatant, shake well and let stand;
[0060] (3) adjust the pH to 6.0 with 10% sodium hydroxide solution and 0.1% sodium hydroxide solution respectively;
[0061] (4) Add 0.5%-1.0% horseradish peroxidase, 0.02-0.08mol / L potassium iodide and 0.08-0.15mol / L 4,4-(dimethylamino)-diphenylmethane solution in turn, shake well After standing;
[0062] (5) Observe the color and compare it with the standard color band to obtain the content of calcium peroxide in the flour.
[0063] Wherein the preparation method of the standard color band is as...
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