A capillary electrophoresis electrochemiluminescence detection method for flavonoids

Abstract

The invention discloses a capillary electrophoresis electrochemiluminescence detection method of a flavonoids compound. The method comprises the following steps: adding acetate buffer liquid into a flavonoids compound standard substance and scanning the spectrum of the standard substance by a spectrophotometer to obtain a control group picture; performing reaction on five amine salts and the flavonoids compound standard substance mother liquor in the acetate buffer liquid separately, scanning a reactant by a spectrophotometer, and comparing a result with the control group picture; adding dipyridyl ruthenium electrochemical luminescence liquid into a capillary electrophoresis electrochemiluminescence detection pond, and detecting an electrochemical luminescence reference map by means of cyclic voltammetry; adding the dipyridyl electrochemical luminescence liquid into the capillary electrophoresis electrochemiluminescence detection pond and adding five solutions, detecting five control groups by means of cyclic voltammetry, and performing comparison to obtain a tertiary amine derived reaction reagent with the strongest electrochemical luminescence signal; and performing follow-up treatment to finish detection. The capillary electrophoresis electrochemiluminescence detection method disclosed by the invention has the advantages that the sample consumption is low and the analyzing result is not interfered by sample oxidization.

Description

technical field [0001] The invention belongs to the technical field of compound detection methods, and in particular relates to a capillary electrophoresis electrochemiluminescence detection method for flavonoid compounds. Background technique [0002] Flavonoids (flavonoids) are a class of compounds that exist in nature and have a 2-phenylchromone (flavone) structure. There is a ketone carbonyl group in their molecules, and the oxygen atom at the first position is basic and can be combined with Strong acid forms a salt, and its hydroxyl derivatives are mostly yellow, so it is also called flavonoid or flavonoid. Flavonoids are usually combined with sugars to form glycosides in plants, and a small part exists in the form of free state (glycon). Most plants contain flavonoids, which play an important role in plant growth, development, flowering, fruiting, antibacterial and disease prevention. There are many compounds with medicinal value in flavonoids, such as: rutin in Xian...

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Problems solved by technology

[0004] As far as the prior art is concerned, the analysis and detection of flavonoids usually use techniques such as ultraviolet spectrophotometry, high performance liquid chromatography, and liquid chromatography-mass spectrometry. However, these methods generally have the problem of large sample consumption, and cannot be used for some Analysis and detection of special and precious trace samples

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