60 results about "Geniposidic acid" patented technology

The invention discloses a folium cortex eucommiae extract which is a water extract or an ethanol extract of folium cortex eucommiae. The folium cortex eucommiae extract contains no less than 9% of chlorogenic acid, no less than 1% of geniposidic acid and no less than 1% of aucubin. Furthermore, the invention also provides a preparation method and an application of the folium cortex eucommiae extract. The folium cortex eucommiae extract, as a traditional Chinese medicine extract, is low in side effect, capable of being used for a long term, free from toxic and side effects to organisms, safe and effective, capable of improving the life quality of patients and having a significant curative effect on psoriasis; and therefore, the folium cortex eucommiae extract has a broad application prospect in treating psoriasis.

The invention discloses a Plantain seed extract and application thereof. The Plantain seed extract is acquired by reflux extraction of Plantain seed with 2-6 volume times of 60-90vol% ethanol aqueous solution 2-6 times. The Plantain seed extract contains acteoside, isoacteoside, geniposidic acid, ferulic acid, and Cheqiansu A and Cheqiansu B. In-vivo experiments show that the Plantain seed extract provided by the invention can promote glucose consumption and has lower toxicity to cells; and simultaneously in-vitro experiments show that the Plantain seed extract can significantly increase serum insulin level and reduce fasting blood-glucose level, presents good medicinal value in treatment of diabetes; therefore, the Plantain seed extract can be used in the preparation of pharmaceutical medicaments for treating diabetes singly or together with a pharmaceutically acceptable composition composed of a drug carrier.

The invention discloses a quality control method of a traditional Chinese medicine compound preparation for treating osteoporosis. The quality control method comprises the following steps: (1) by taking eucommia ulmoides as a contrast medicinal material and taking pinoresinol diglucoside and geniposidic acid as comparison products, carrying out qualitative identification on eucommia ulmoides in the traditional Chinese medicine compound preparation by adopting a thin-layer chromatography; (2) by taking radix achyranthis bidentatae as a contrast medicinal material and taking beta-ecdysterone as a comparison product, carrying out qualitative identification on radix achyranthis bidentatae in the traditional Chinese medicine compound preparation by adopting the thin-layer chromatography; (3) by taking wolfberry fruits as contrast medicinal materials, carrying out qualitative identification on the wolfberry fruits in the traditional Chinese medicine compound preparation by adopting the thin-layer chromatography; and (4) determining the content of three active components (or index components) including epimedium glucoside, gentiopicroside and loganin acid in the traditional Chinese medicine compound preparation by adopting a high performance liquid chromatography. According to the quality control method, the quality of the traditional Chinese medicine compound preparation can be controlled very well, and the stability of a product production process is monitored; and the quality of the traditional Chinese medicine compound preparation is stable, uniform and controllable.

Disclosed are a eucommia leaf extract, and preparation method and use thereof. The extract is a liquid of the eucommia leaf or an ethyl alcohol extract, and has chlorogenic acid of a content not less than 9%, geniposidic acid of a content not less than 1% and aucubin of a content not less than 1%. The extract has a good effect on psoriasis.

The invention discloses eucommia ulmoides fine powder and a preparation method thereof. The eucommia ulmoides fine powder comprises the following components: larger than or equal to 15 percent of total sugar, larger than or equal to 5 percent of chlorogenic acid, larger than or equal to 15 percent of total flavone, larger than or equal to 1.8 percent of geniposidic acid, larger than or equal to 0.8 percent of aucubin, less than or equal to 6.0 percent of water and trace elements such as B, Ca, Cu, Mg, Mn, P, Se, Zn, Na, K and Fe. According to the preparation method, the eucommia ulmoides fine powder is efficiently extracted from eucommia ulmoides leaves and eucommia ulmoides bark and a membrane technology is introduced for separation, concentration, decoloration and the like, so that the labor and time are reduced, the product quality and yield can also be improved, the temperature of the extraction process is low, and the damage of high temperature heating on effective components is avoided. In addition, the phenomenon that the eucommia ulmoides fine powder is insoluble in water the second time is avoided. The eucommia ulmoides fine powder belongs to a natural health food preparation, is safe and non-toxic, and is suitable for the industries such as medicine, health care products and food antioxidants. Eucommia ulmoides gum can be continuously extracted out from extractive residue after extraction of the eucommia ulmoides fine powder, and the economic benefit of high-value using of eucommia ulmoides is effectively improved.

The invention belongs to the field of extraction of traditional Chinese medicine, and particularly relates to a cortex eucommiae extract composition for treating renal fibrosis and application of thecortex eucommiae extract composition. The cortex eucommiae extract composition is prepared from the components of aucubin, a mixed extract of geniposide and geniposidic acid, quercetin and chlorogenicacid through the steps of extraction, separation, purification and the like, and the cortex eucommiae extract composition can be further prepared into a preparation. A preparation method is simple and efficient, and the prepared cortex eucommiae extract composition has the good effect of treating renal fibrosis.

The invention belongs to the technical field of pomegranate planting and particularly provides a cutting seedling-raising matrix for pomegranates. The cutting seedling-raising matrix is prepared from perlite, river sand, rotten leaf soil, fresh cow dung, compound fermentation bacteria, rice bran, soybean meal, fresh wax gourd peel, pepper leaves, fresh semen pharbitidis extract, litchi kernel extract, lignan, geniposidic acid and anaphalis xylorhiza alkali. The cutting seedling-raising matrix is comprehensive and sufficient in nutrition, good in permeability, strong in biological activity, good in slow-release property and long in acting time, does not need fertilization in a whole seedling-raising period, only needs watering, is simple to use, makes cuttings root quickly, makes stems and leaves grow healthily and strongly, improves the disease resistance ability of the cuttings, reduces plant diseases and insect pests and improves the seedling-raising quality of cuttings, and a rejuvenation period after transplantation is shortened. The cutting seedling-raising matrix makes the cutting rooting time shortened to be 13.6-14.1 days, the rooting rate is up to 97.8%-98.1%, and the survival rate is up to 96.3%-96.7%.

The invention discloses a method for preparing eucommia ulmoides extract by using a natural eutectic solvent. The method comprises the following steps that: eucommia ulmoides powder is added into the natural eutectic solvent according to a material-liquid ratio of (1: 1)-(1: 100g/mL), and ultrasonic-assisted extraction is performed for 10-90 minutes at 20-80 DEG C and 50-500W, wherein the natural eutectic solvent is composed of a hydrogen bond donor, a hydrogen bond acceptor and distilled water according to the mass ratio of 1: (1-30): (1-50), wherein the hydrogen bond donor comprises L-lactic acid, sorbitol, urea, DL-malic acid, D-fructose, glucose and citric acid, and the hydrogen bond acceptor comprises choline chloride, betaine and citric acid; centrifugation is performed, supernatant is taken, and the supernatant is further concentrated and dried, so that the eucommia ulmoides extract is obtained. The method is safe, economical and recoverable; various active ingredients such as aucubin, geniposidic acid, chlorogenic acid and rutin in eucommia ulmoides can be extracted; and compared with that of a water extract, the content of the active ingredients in the natural eutectic solvent extract is remarkably increased, the extraction efficiency is high, and the components of the natural eutectic solvent can also be used as functional components in functional food and cosmetics; and the step of removing an extraction solvent in a traditional process is omitted. The method is more economical and efficient.

The invention discloses a method for simultaneously measuring the content of multiple active ingredients in eucommia ulmoides. The method adopts high performance liquid chromatography for analysis; the chromatographic condition is taking octadecylsilane bonded silica as a filler; the mobile-phase gradient elution process is: the mobile phase A is methanol, and the mobile phase B is an aqueous solution of phosphoric acid with mass concentration of 0.1%; the content of the mobile phase B is increased from 30% to 70% by mass according to the first-order linear gradient in 0.01-30.00min, reduced to 30% in 30.00-30.01min and kept at 30% in 30.01-40.00min; the flow rate is 0.8ml/min; the column temperature is 30 DEG C; the sample injection volume is 5mul; the detection wavelength detection process is: the 254nm wavelength is selected in 0.01-7.00min, the 277nm wavelength is selected in 7.00-12.50min, and the 254nm wavelength is selected in 12.50-40.00min; and multiple active ingredients include geniposidic acid, chlorogenic acid, pinoresinol diglucoside and rutin. According to the invention, the method is verified by linear relation survey, stability test, precision test, reproducibility test and sample injection recycling test, and the analysis method is proved to be accurate and reliable.

The invention relates to the field of tattoo stickers, in particular to a water transfer printing tattoo sticker as well as a preparation method and a use method thereof. The water transfer printing tattoo sticker is sequentially provided with four layers of structures, wherein the first layer is water transfer printing paper, the second layer is SD glue, the third layer is an adhesive mixture containing genipin or geniposidic acid, and the fourth layer is a protective film. The water transfer printing tattoo sticker has the advantages that firstly, use is convenient as the tattoo sticker needs to be attached to a certain portion of the surface of a body only, bottom paper is taken out after 1 minute, and color can be developed after 2-10h; secondly, the water transfer printing tattoo sticker is nontoxic and harmless, since the genipin and the geniposidic acid, as natural compounds, are adopted as chromogenic substances, and a large number of experiments show the safety of the genipinand the geniposidic acid; thirdly, the water transfer printing tattoo sticker is painless and non-invasive; and fourthly, the color of a pattern is long in retention time, the pattern is relatively intuitive, does not fade with being kept for 30 days in winter and does not fade with being kept for 10 days or above in summer, and the water transfer printing tattoo sticker is more washable relativeto a common tattoo sticker.

The invention discloses a degradable composite nanofiber three-dimensional material and application thereof in tissue repair, and belongs to the technical field of degradable materials. The compositenanofiber three-dimensional material is prepared from a dopamine graft, and the dopamine graft adopts geniposidic acid to graft dopamine. The dopamine graft is adopted to prepare the composite nanofiber three-dimensional material so that the total amount of cell DNA on the surface of the composite nanofiber three-dimensional material is increased by at least 42.86%, and the highest dissolution loss rate of the composite nanofiber three-dimensional material is only 11%. The composite nanofiber three-dimensional material can be used for tissue repair materials and is stable, porous, good in biocompatibility and low in dissolution loss rate.

The invention discloses a method of rapidly identifying three active ingredients of Eucommia ulmoides Oliv leaves and barks by using thin-layer chromatography. The method comprises the following stepsof: 1) respectively preparing aucubin, geniposidic acid and chlorogenic acid reference substance solutions, 2) respectively preparing to-be-detected sample solutions of Eucommia ulmoides Oliv leavesand barks, and 3) respectively sucking proper amounts of the reference substance solutions and the to-be-detected sample solutions by using a quantitative capillary tube, applying the solutions on a GF 254 silica gel plate, taking butyl acetate: methanol: formic acid as a mixed solvent as a developing solvent, observing through a 254 nm ultraviolet lamp, carrying out thin-layer chromatography identification by taking a 5.0% sulfuric acid-ethanol solution as a color developing agent, and observing the positions and the colors of spots appearing in the thin-layer chromatography silica gel plateof the sample solution. Results show that three active ingredients of Eucommia ulmoides Oliv leaves and barks in an obtained chromatogram show different spots. The identification method is high in specificity and convenient to operate, and can be used for rapidly and accurately identifying aucubin, geniposidic acid and chlorogenic acid in Eucommia ulmoides Oliv leaves and barks at the same time.

The invention relates to a gardenia extract, in particular to a gardenia extract for treating hyperbileacidemia. The gardenia extract comprises geniposide, genipin 1-beta-D-gentiobioside, 6''-p-coumaroyl genipin gentiobioside, scandoside methyl ester, geniposidic acid, gardenoside and gardenoside, and is prepared through ethanol extraction and macroporous resin purification. The gardenia extract is capable of significantly reducing the bile acid level in the blood of rats, has a good effect on the treatment of hyperbileacidemia, and can be applied to the preparation of drugs for treating hyperbileacidemia.

The invention discloses a structural formula of an LQC-Y as well as synthesis and an application thereof. Pharmacological experiments verify that the compound has a remarkable anti-tumor effect, wherein the single day maximum dosage of an LQC-Y3 mouse is 6000mg/kg. No toxic reactions happen within 14 days of continuous observation, thus, the drug is very high in safety and can be used for preparing drugs for preventing and treating cancers such as liver cancer and lung cancer. The structural formula of LQC-Y is as shown in the specification, wherein R represents sterides such as cholic acid, deoxycholic acid, ursodesoxycholic acid, chenodeoxycholic acid and hyodeoxycholic acid; triterpenoids such as oleanolic acid, ursolic acid, pachymic acid, glycyrrhetinic acid and glycosides; parietic acid, rheum emodin and other anthraquinone mother nucleus mono-substituted or multi-substituted structures; baicalein, baicalin and other flavone analogue compounds; shikimic acid, mono-substituted shikimic acid or multi-substituted shikimic acid; geniposidic acid and other iridoid acid derivatives; and paeonol, curcumin and structural derivatives.

The invention belongs to the technical field of preparation of novel compounds, and particularly relates to an amide pentaacetyl geniposide derivative as well as a preparation method and application thereof. The amide penta-acetyl geniposide derivative provided by the invention is prepared by the following steps: firstly, hydrolyzing lipid groups at C-4 sites of iridoid in geniposide to obtain geniposidic acid, then acetylating five hydroxyl groups at 2 ', 3', 4 ', 6' and 11 sites on sugar to obtain penta-acetyl geniposidic acid (2a), and finally reacting with RNH2 to obtain the penta-acetyl geniposide derivative. The geniposide derivative prepared by the invention has remarkable XOD inhibitory activity and kidney injury improvement activity, and can effectively reduce the accumulation of uric acid in the kidney. The inflammatory reaction is reduced, so that the level of inflammatory factors is reduced; renal fibrosis of a mouse with hyperuricemia can be reduced by inhibiting expression of an inflammatory factor TGF-beta.

The invention discloses a method for measuring the concentration of 7 blood inflow ingredients in an eucommia extract. The method comprises the following steps: firstly preparing a standard control solution by using geniposidic acid, protocatechuic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, pinoresinol diglucoside and terpineol monoglucoside; preparing an internal standard solution by using puerarin; then establishing an analysis method for simultaneous measuring the concentration of 7 ingredients in the eucommia extract in rat plasma by using an HPLC-ESI-MS/MS analysis; and the result shows that the measurement method disclosed by the invention has the advantages of wide linear range, high sensitivity and low limit of quantification, and can be used for accurately and reliably measuring the concentration of the 7 ingredients in the eucommia extract in the rat plasma extracted at 15 different time points after a spontaneously hypertensive rat is gavaged with the eucommia extract at the same time, and can be used for providing a technical support for the researches on the pharmacokinetic characteristics of the 7 ingredients in the eucommia extract.

The invention discloses a method for extracting geniposidic acid and verbascoside in semen plantaginis by an ASE (Accelerated Solvent Extraction) method. The method is shorter in extraction time and good in repeatability, and belongs to the field of extraction of the geniposidic acid and the verbascoside. The method comprises the following steps: crushing a semen plantaginis sample, enabling the crushed semen plantaginis sample to pass through a second sieve, taking 1g of the semen plantaginis sample powder and mixing the 1g of the semen plantaginis sample powder with 1g of diatomite; charging a mixture obtained in the first step into an ASE extraction pool in which a fiber filtration film is putted, and adding the diatomite to be parallel to a pool opening; extracting with methanol, and fixing the volume of an extracting solution to be 50ml by using the methanol. The method disclosed by the invention can replace a pharmacopoeia method for extracting the geniposidic acid and the verbascoside in the semen plantaginis and determining the contents.

The invention belongs to the technical field of novel compound preparation, and particularly relates to an ester pentaacetyl geniposide derivative obtained through esterification reaction and a preparation method and application thereof. The amide penta-acetyl geniposide derivative provided by the invention is prepared by the following steps: firstly, hydrolyzing lipid groups at C-4 sites of iridoid in geniposide to obtain geniposidic acid, then acetylating five hydroxyl groups at 2 ', 3', 4 ', 6' and 11 sites on sugar to obtain penta-acetyl geniposidic acid (2a), and finally reacting with ROH to obtain the penta-acetyl geniposide derivative. The geniposide derivative prepared by the invention has remarkable XOD inhibitory activity and kidney injury improvement activity, and can effectively reduce the accumulation of uric acid in the kidney. The inflammatory reaction is reduced, so that the level of inflammatory factors is reduced; renal fibrosis of a mouse with hyperuricemia can be reduced by inhibiting expression of an inflammatory factor TGF-beta.