Method for measuring concentration of 7 blood inflow ingredients in Eucommia ulmoides extract
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A technology for determination of blood components and concentrations, applied in the field of traditional Chinese medicine research, can solve the problems of few detection components and low sensitivity, and achieve the effects of low quantitative limit, high sensitivity and wide linear range.
Inactive Publication Date: 2019-07-26
GUIZHOU MEDICAL UNIV
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The current research on the components of Eucommia enters the blood, or the sensitivity is too low, or the detec...
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[0014] In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with examples.
[0015] Embodiments of the invention:
[0016] 1. Instruments and materials
[0017] 1.1 Instrument
[0018] API 5500Q-TRAP high performance liquid chromatography-triple quadrupole tandem mass spectrometer (AB-SCIEX system, Analyst Software workstation), MRBP noninvasive blood pressure monitor (US IITC company), VX-Ⅲ multi-tube vortex oscillator ( Beijing Fujijin Pharmaceutical Technology Co., Ltd.), Allegra64R low-temperature high-speed centrifuge (Beckman Coulter, USA), KQ-300DE ultrasonic cleaning machine (Guizhou Zhongrun Stone Technology Co., Ltd.), NA-5L nitrogen-air all-in-one machine (Beijing Zhongxing Huili Science and Technology Development Co., Ltd.), WP-UP-II-20 analytical ultrapure water machine (Sichuan Water Technology Development Co., Ltd.), EL204 1 / 10,000 electr...
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Abstract
The invention discloses a method for measuring the concentration of 7 blood inflow ingredients in an eucommia extract. The method comprises the following steps: firstly preparing a standard control solution by using geniposidic acid, protocatechuic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, pinoresinol diglucoside and terpineol monoglucoside; preparing an internal standard solution by using puerarin; then establishing an analysis method for simultaneous measuring the concentration of 7 ingredients in the eucommia extract in rat plasma by using an HPLC-ESI-MS/MS analysis; and the result shows that the measurement method disclosed by the invention has the advantages of wide linear range, high sensitivity and low limit of quantification, and can be used for accurately and reliably measuring the concentration of the 7 ingredients in the eucommia extract in the rat plasma extracted at 15 different time points after a spontaneously hypertensive rat is gavaged with the eucommia extract at the same time, and can be used for providing a technical support for the researches on the pharmacokinetic characteristics of the 7 ingredients in the eucommia extract.
Description
technical field [0001] The invention relates to a method for measuring the concentration of seven components entering blood in Eucommia ulmoides extract, belonging to the technical field of traditional Chinese medicine research. Background technique [0002] Eucommia ulmoides, also known as Sixian, Sizhong, and kapok, is a unique medicinal plant in my country and a second-class rare protected plant in the country. It is mainly distributed in Gansu, Shaanxi, Henan, Hubei, Shanxi, Hunan, Sichuan, and Yunnan. In the provinces of China, Guizhou, Du, Fujian, etc., its skin, stems, and leaves can be used as medicine. Its traditional pharmacological effects are "invigorating the liver and kidney, strengthening bones and muscles, lowering blood pressure, etc." Modern medical research shows that Eucommia has the functions of lowering blood pressure, anti-aging, anti-oxidation, anti-tumor, anti-HIV activity, anti-osteoporosis, antibacterial and anti-inflammatory, inhibiting bone cell p...
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