Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for measuring concentration of 7 blood inflow ingredients in Eucommia ulmoides extract

A technology for determination of blood components and concentrations, applied in the field of traditional Chinese medicine research, can solve the problems of few detection components and low sensitivity, and achieve the effects of low quantitative limit, high sensitivity and wide linear range.

Inactive Publication Date: 2019-07-26
GUIZHOU MEDICAL UNIV
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current research on the components of Eucommia enters the blood, or the sensitivity is too low, or the detection components are few, which cannot meet the requirements of characterizing the overall pharmacokinetics of Eucommia

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for measuring concentration of 7 blood inflow ingredients in Eucommia ulmoides extract
  • Method for measuring concentration of 7 blood inflow ingredients in Eucommia ulmoides extract
  • Method for measuring concentration of 7 blood inflow ingredients in Eucommia ulmoides extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with examples.

[0015] Embodiments of the invention:

[0016] 1. Instruments and materials

[0017] 1.1 Instrument

[0018] API 5500Q-TRAP high performance liquid chromatography-triple quadrupole tandem mass spectrometer (AB-SCIEX system, Analyst Software workstation), MRBP noninvasive blood pressure monitor (US IITC company), VX-Ⅲ multi-tube vortex oscillator ( Beijing Fujijin Pharmaceutical Technology Co., Ltd.), Allegra64R low-temperature high-speed centrifuge (Beckman Coulter, USA), KQ-300DE ultrasonic cleaning machine (Guizhou Zhongrun Stone Technology Co., Ltd.), NA-5L nitrogen-air all-in-one machine (Beijing Zhongxing Huili Science and Technology Development Co., Ltd.), WP-UP-II-20 analytical ultrapure water machine (Sichuan Water Technology Development Co., Ltd.), EL204 1 / 10,000 electr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for measuring the concentration of 7 blood inflow ingredients in an eucommia extract. The method comprises the following steps: firstly preparing a standard control solution by using geniposidic acid, protocatechuic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, pinoresinol diglucoside and terpineol monoglucoside; preparing an internal standard solution by using puerarin; then establishing an analysis method for simultaneous measuring the concentration of 7 ingredients in the eucommia extract in rat plasma by using an HPLC-ESI-MS / MS analysis; and the result shows that the measurement method disclosed by the invention has the advantages of wide linear range, high sensitivity and low limit of quantification, and can be used for accurately and reliably measuring the concentration of the 7 ingredients in the eucommia extract in the rat plasma extracted at 15 different time points after a spontaneously hypertensive rat is gavaged with the eucommia extract at the same time, and can be used for providing a technical support for the researches on the pharmacokinetic characteristics of the 7 ingredients in the eucommia extract.

Description

technical field [0001] The invention relates to a method for measuring the concentration of seven components entering blood in Eucommia ulmoides extract, belonging to the technical field of traditional Chinese medicine research. Background technique [0002] Eucommia ulmoides, also known as Sixian, Sizhong, and kapok, is a unique medicinal plant in my country and a second-class rare protected plant in the country. It is mainly distributed in Gansu, Shaanxi, Henan, Hubei, Shanxi, Hunan, Sichuan, and Yunnan. In the provinces of China, Guizhou, Du, Fujian, etc., its skin, stems, and leaves can be used as medicine. Its traditional pharmacological effects are "invigorating the liver and kidney, strengthening bones and muscles, lowering blood pressure, etc." Modern medical research shows that Eucommia has the functions of lowering blood pressure, anti-aging, anti-oxidation, anti-tumor, anti-HIV activity, anti-osteoporosis, antibacterial and anti-inflammatory, inhibiting bone cell p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/045
Inventor 巩仔鹏张楠吴林霖李月婷王广成李梅胡贺佳肖红琴陈思颖郑林王爱民黄勇王永林刘丽娜
Owner GUIZHOU MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com