64 results about "Pinoresinol diglucoside" patented technology

The invention discloses a method for extraction and separation of chlorogenic acid, pinoresinol diglucoside, aucubin and gutta-percha from a eucommia ulmodies oliv raw material. The method comprises the steps: extracting from the eucommia ulmodies oliv raw material by an organic solvent, concentrating, successively carrying out hot water dissolving, cold precipitation and centrifugal separation on the concentrate to obtain a centrifugal liquid, carrying out enrichment, elution and concentration on the centrifugal liquid, to obtain an eucommia ulmodies oliv extract, separating and purifying the eucommia ulmodies oliv extract, and thus respectively obtaining the pinoresinol diglucoside, chlorogenic acid and aucubin; and carrying out hydrolysis and organic solvent extraction on the extract residue, to obtain the gutta-percha. The method extracts and separates the high-purity chlorogenic acid, pinoresinol diglucoside, aucubin and gutta-percha from the eucommia ulmodies oliv with relatively high yield simultaneously, has the advantages of simple operation and low cost, realizes the comprehensive utilization of natural resources, and meets industrialized production.

The invention discloses a quality control method of a traditional Chinese medicine compound preparation for treating osteoporosis. The quality control method comprises the following steps: (1) by taking eucommia ulmoides as a contrast medicinal material and taking pinoresinol diglucoside and geniposidic acid as comparison products, carrying out qualitative identification on eucommia ulmoides in the traditional Chinese medicine compound preparation by adopting a thin-layer chromatography; (2) by taking radix achyranthis bidentatae as a contrast medicinal material and taking beta-ecdysterone as a comparison product, carrying out qualitative identification on radix achyranthis bidentatae in the traditional Chinese medicine compound preparation by adopting the thin-layer chromatography; (3) by taking wolfberry fruits as contrast medicinal materials, carrying out qualitative identification on the wolfberry fruits in the traditional Chinese medicine compound preparation by adopting the thin-layer chromatography; and (4) determining the content of three active components (or index components) including epimedium glucoside, gentiopicroside and loganin acid in the traditional Chinese medicine compound preparation by adopting a high performance liquid chromatography. According to the quality control method, the quality of the traditional Chinese medicine compound preparation can be controlled very well, and the stability of a product production process is monitored; and the quality of the traditional Chinese medicine compound preparation is stable, uniform and controllable.

The invention relates to a processing method of salt eucommia bark slices. The characteristics include the following steps: raw eucommia bark is took and is cut into coarse silks, salt is added to mix up (2Kg salt per 100Kg medicinal materials), wrap-moisten is carried out for 1.0 to 2.0 hours, which is put into the pot under 175-185 DEG C and is stirred for 5 to10 minutes until the rate of broken silks reaches 80 to 90% and the surface is black char color, and is took out and cooled. The invention quantizes traditional fuzzy processing methods by determining the wrap-moistening time, stirring temperature and time, which is convenient for processing. The processed salt eucommia bark with the invention contains more than 1.0 percent of pinoresinol diglucoside that is 5-10 times to the traditional method.

The invention discloses a method for simultaneously measuring the content of multiple active ingredients in eucommia ulmoides. The method adopts high performance liquid chromatography for analysis; the chromatographic condition is taking octadecylsilane bonded silica as a filler; the mobile-phase gradient elution process is: the mobile phase A is methanol, and the mobile phase B is an aqueous solution of phosphoric acid with mass concentration of 0.1%; the content of the mobile phase B is increased from 30% to 70% by mass according to the first-order linear gradient in 0.01-30.00min, reduced to 30% in 30.00-30.01min and kept at 30% in 30.01-40.00min; the flow rate is 0.8ml/min; the column temperature is 30 DEG C; the sample injection volume is 5mul; the detection wavelength detection process is: the 254nm wavelength is selected in 0.01-7.00min, the 277nm wavelength is selected in 7.00-12.50min, and the 254nm wavelength is selected in 12.50-40.00min; and multiple active ingredients include geniposidic acid, chlorogenic acid, pinoresinol diglucoside and rutin. According to the invention, the method is verified by linear relation survey, stability test, precision test, reproducibility test and sample injection recycling test, and the analysis method is proved to be accurate and reliable.

The invention discloses a method for testing the content of pinoresinol diglucoside in a compound eucommia bark tablet, which belongs to a method of testing the content of an active ingredient of a medicament in the medicine field. Testing by high performance liquid chromatography, the content of the pinoresinol diglucoside in each compound eucommia bark tablet is not less than 0.05mg, the basic weight of each tablet is 0.35g, in particular octadecyl silane bonded silica is used as filler; methanol is used as a mobile phase A and water is used as a mobile phase B to carry out gradient elution; the detection wavelength is 230mum and the flow rate is 1ml/min; the theoretical plate number in the term of the pinoresinol diglucoside is not less than 2000; and20mul of reference substance solution and testing solution are respectively accurately sucked, and injected into a high performance liquid chromatograph, tested and calculated according to an external standard method to obtain the content of the pinoresinol diglucoside. The method for testing the content of the pinoresinol diglucoside has high sensitivity, and proved by an experiment, the accuracy, reproducibility, linearity, stability of the method are all meet the requirements.

The invention relates to the field of purposes of extracts of traditional Chinese medicine, in particular to application of eucommia ulmoides lignans extracts in preparation of medicine treating renal interstitial fibrosis. Lignans accounts for 30-90% of the total weight of the eucommia ulmoides lignans extracts, and the effective constituent of pinoresinol diglucoside in the lignans accounts for 1%-15% of the total weight of the eucommia ulmoides lignans extracts.

The invention specifically relates to a comprehensive utilization method for eucommia bark, which belongs to extraction methods for effective ingredients of plants. The comprehensive utilization method for the eucommia bark improves the utilization rate of raw materials and reduces production cost. The method comprises the following steps: with the eucommia bark as a raw material, crushing the eucommia bark, adding water for extraction and then carrying out solid-liquid separation; feeding a separated liquid into a macroporous resin adsorption column, carrying out desorption by using ethanol and subjecting a desorption solution to concentration and drying so as to obtain dry powder containing pinoresinol diglucoside; and cyclically extracting a separated solid with No. 120 solvent naphtha or toluene, removing impurities by using ethyl acetate until a solvent is colorless and carrying out air drying so as to obtain refined gutta-percha. According to the invention, pinoresinol diglucoside soluble in water and gutta-percha insoluble in water are extracted, so product cost is greatly reduced.

The invention belongs to the technical field of traditional Chinese medicine preparation, and particularly relates to a composition containing pinoresinol diglucoside and genipin and application thereof. The composition containing pinoresinol diglucoside and genipin is prepared from the following components: pinoresinol diglucoside, genipin, nicotinamide, quercetin, a radix achyranthis bidentatae extract, tartronic acid and citric acid. The composition is simple in preparation method, can be used for treating hypertensive nephropathy, has a good curative effect, and can reduce side effects and reduce cost.

The invention provides a preparation method for standardized extracts of eucommia ulmoides pinoresinol diglucoside. The method comprises the following steps: crushing dry eucommia ulmoides skin raw materials into 20-80 mesh, extracting for 1-2h according to material-liquid ratio of 1:(10-15), performing solid-liquid separation by using a centrifuge, performing micropore refined filtration, enabling the refined filtration liquid to pass through an ultrafiltration membrane to be separated, adsorbing by using macroporous adsorptive resin, eluting, concentrating the eluent, performing column chromatography by using C18 reverse phase silica gel for purification, performing secondary chromatography on the chromatography eluent by using polyamide columns and the like for purification, performing vacuum concentration on the secondary chromatography eluent, and then freeze-drying, so as to obtain eucommia ulmoides pinoresinol diglucoside standardized extracts at different purity levels. The preparing method is high in extracting efficiency and wide in technological applicability, can be used for producing standardized extracts at different purity levels according to different consumer market requirements of eucommia ulmoides health products, besides, facilitates the reduction of use amount of organic solvents, saves energy, reduces carbon emission, and reduces production cost and environmental pollution.

The invention discloses a thin-layer chromatography detection method of a traditional Chinese medicine composition. The thin-layer chromatography detection method comprises the following steps: a, preparing a test solution; b, preparing a reference solution; c, respectively applying the test solution, the reference solution of astragaloside, the reference solution of oleanolic acid and the reference solution of pinoresinol diglucoside to the same silica gel plate, developing by taking the lower solution of chloroform-ethyl acetate-methanol-water as a developing agent, taking out, airing, and heating by taking 2% vanillic aldehyde sulfuric acid solution as a colour developing agent till the colour of spots is developed clearly; and d, viewing the silica gel plate after the colour is developed. The thin-layer chromatography detection method of the traditional Chinese medicine composition disclosed by the invention can be used for simultaneously detecting astragalus membranaceus, glossy privet fruits and the bark of eucommia in the traditional Chinese medicine composition; the detection result is accurate and reliable; and simultaneously, the thin-layer chromatography detection method disclosed by the invention has the advantages of being few in process, simple and convenient to operate, short in detection time, low in cost and the like and is particularly applied to industrial application.

The invention belongs to the field of traditional Chinese medicines and specifically relates to a pharmaceutical composition derived from eucommia ulmoides and a use of the pharmaceutical composition. The pharmaceutical composition is prepared from the following components in percentage by weight: 5-60% of pinoresinol diglucoside, 5-60% of eucommiae ulmoides glycoside, 5-60% of ferulic acid and 5-60% of betulin, preferably, 30-55% of pinoresinol diglucoside, 10-30% of eucommiae ulmoides glycoside, 10-30% of ferulic acid and 20-40% of betulin. After years of experiment research and clinical practice, a pharmaceutical composition having obvious curative effect on osteoporosis is screened from the active compounds in the eucommia ulmoides. Besides, the osteoporosis treatment effect of the composition is improved to be better and more remarkable by virtue of reasonable optimization of matching by weight in combination with the modern science and technology; as a result, the pharmaceutical composition derived from the eucommia ulmoides has a tremendous clinical development value.

The invention claims a method of preparing pinoresinol diglucoside by separating endophyte from Eucommia ulmoides Oliver by a microorganism fermentation method. The endophyte is XP-8 and deposited in China Center for Type Microorganism Collection under a number of CCTCCM209291. The preparation method comprises steps of preparing and fermenting liquid seeds; separating, extracting and purifying a product. The method is simple, fast and low in cost.

The invention discloses a method for measuring the concentration of 7 blood inflow ingredients in an eucommia extract. The method comprises the following steps: firstly preparing a standard control solution by using geniposidic acid, protocatechuic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, pinoresinol diglucoside and terpineol monoglucoside; preparing an internal standard solution by using puerarin; then establishing an analysis method for simultaneous measuring the concentration of 7 ingredients in the eucommia extract in rat plasma by using an HPLC-ESI-MS/MS analysis; and the result shows that the measurement method disclosed by the invention has the advantages of wide linear range, high sensitivity and low limit of quantification, and can be used for accurately and reliably measuring the concentration of the 7 ingredients in the eucommia extract in the rat plasma extracted at 15 different time points after a spontaneously hypertensive rat is gavaged with the eucommia extract at the same time, and can be used for providing a technical support for the researches on the pharmacokinetic characteristics of the 7 ingredients in the eucommia extract.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a eucommia lignanoid preparation and a preparation method of the eucommia lignanoid preparation. The eucommia lignanoid preparation comprises the following raw materials in percentage by weight: 5%-20% of pinoresinol diglucoside, 5%-15% of syringaresinol diglucoside, 1%-10% of pinoresinol A diglucoside, 5%-15% of olivil biglucoside, and 1%-5% of naringenin B, wherein a eucommia lignanoid extract preferably contains 10%-15% of pinoresinol diglucoside, 8%-12% of syringaresinol diglucoside, 5%-8% of pinoresinol A diglucoside, 8%-12% of olivil biglucoside and 2%-4% of naringenin B.

The invention relates to an eucommia bark processing method, which comprises the following steps that (1) raw medicinal materials are taken; impurities, foreign matters and non-medicinal parts in the medicinal materials are picked out; the rest materials are put into a clean container; and the impurity content is less than or equal to 2.0 percent; (2) according to the proportion that a mass ratio of eucommia barks to fresh ginger is 100:(12 to 20), the fresh ginger is firstly squeezed to obtain ginger juice; then, water is added for dilution to reach the mass being 1.5 to 2.5 times of the original mass; then, the ginger juice is added into the eucommia barks; uniform stirring is performed; the mixture is fully moistened; and the content of the non-moistened eucommia barks is less than or equal to 10 percent; (3) after the ginger juice is completely absorbed; the mixture is put into a stir-frying container and is stir-fried for 15 to 25 min by strong fire at 150 to 250 DEG C; and the mixture is taken out; (4) the mixture is dried or put into an electric heating constant temperature blasting drying box; and the drying is performed at the temperature of 60 to 70 DEG C; and (5) powder is sieved away by a pharmacopeia sieve, and the powder content is less than or equal to 3.0 percent. The contents of pinoresinol diglucoside and extracts in ginger and eucommia bark products conform to pharmacopeia standards; and the efficacies of warming and invigorating kidney yang, invigorating the spleen and the stomach and strengthening the bones are achieved.

The invention provides a eucommia ulmoides leaf pretreatment method for extracting pinoresinol diglucoside from eucommia ulmoides leaves, and the method comprises the following steps: 1) low-temperature drying: under a vacuum freezing condition, drying fresh leaves at a first temperature T1 to obtain dried anhydrous leaves; (2) low-temperature wire breaking: shearing the dried anhydrous leaves into sheets, and freezing at a second temperature T2 for wire breaking treatment; 3) low-temperature crushing: crushing the broken leaves at a third temperature T3 to obtain brown green eucommia ulmoides leaf powder, wherein the third temperature T3 is less than 10 DEG C, and the third temperature T3 > the second temperature T2 > the first temperature T1. Through low temperature of three stages, the maintenance of effective components in the leaves is ensured.

The invention discloses a cortex eucommiae fresh extract spray drying granular decoction piece. The cortex eucommiae fresh extract spray drying granular decoction piece is mainly characterized in thatafter cortex eucommiae is harvested, when the cortex eucommiae is fresh, washing, slicing, juicing and extracting are conducted, after an extracting solution is extracted, spray drying is conducted,and pelletizing, granulation and vacuum packaging are conducted. The water content of the granular decoction piece is smaller than 10%, and pinoresinol diglucoside contained in a finished product is not lower than 0.20%. The decoction piece is stable in quality, can be put in a decoction for decoction and can also be directly put in capsules and tablets, carrying is facilitated, and convenience isbrought to clinical application.

The invention belongs to the technical field of medicine preparation, and particularly relates to a composition containing pinoresinol diglucoside and a preparation method and application of the composition. The composition containing the pinoresinol diglucoside is prepared from the following components: the pinoresinol diglucoside, syringaresinol, agaric polysaccharide, gamma-aminobutyric acid, manninotriose and fingered citron lactone. The composition has a good effect of treating osteoporosis, the preparation method is simple, the toxic and side effects are small, and the cost is low.

The invention relates to the technical field of medicines, in particular to an application of eucommia ulmoides lignan extract in preparation of a PH (pulmonary hypertension) treatment drug. Lignans in the eucommia ulmoides lignan extract account for 30%-90% of the total weight of the eucommia ulmoides lignan extract, and the content of an effective ingredient, namely pinoresinol diglucoside, in the lignans accounts for 1%-15% of the total weight of the eucommia ulmoides lignan extract.

The invention relates to a processing method of a traditional Chinese medicine folium eucommiae. The processing method comprises the following steps: wetting the folium eucommiae with table salt water; after completely wetting the folium eucommiae, putting the folium eucommiae into a drying oven and drying. According to the processing method, technological technical parameters of processing the folium eucommiae are defined, the defect of sensory evaluation of the quality is changed; the stable quality of salt-processed folium eucommiae decoction pieces is ensured; the highest content of pinoresinol diglucoside reaches 0.2798 percent; the highest content of chlorogenic acid reaches 0.6483 percent; the highest content of total flavonoids reaches 7.6289 percent; when the technology provided by the invention is used for processing the folium eucommiae, the quality is stable and the expression of a clinical curative effect of the folium eucommiae can be effectively ensured.

The invention discloses a method for determining pinoresinol diglucoside in eucommia ulmoides, belongs to the field of chemical detection, and aims to provide the method for determining the pinoresinol diglucoside in the eucommia ulmoides, wherein the method has an impurity-removing step same as that of the pharmacopoeia, only uses ASE to replace the extraction process to shorten the pretreatmenttime, and at the same time ensures the accuracy of the determination. A crushed eucommia ulmoides sample is extracted by an ASE extraction method, wherein the ASE extraction method includes methanol extraction in a Soxhlet extractor, methanol extraction in a rapid solvent extraction apparatus, and collection of a methanol extract; a HPLC method is used to detect the pinoresinol diglucoside in themethanol extract. The method is used for determination of the pinoresinol diglucoside in the eucommia ulmoides.

The invention discloses a method for determination of pinoresinol diglucoside in Eucommia ulmoides by an ASE-HPLC method and belongs to the field of chemical detection. The method utilizes an impurity removal process in the pharmacopeia, utilizes ASE to replace an extraction process, shortens pretreatment time and guarantees determination accuracy. A crushed sample of Eucommia ulmoides is extracted by an ASE extraction method. The ASE extraction method comprises extraction based on trichloromethane in a Soxhlet extractor, extraction based on methanol in a fast solvent extractor, methanol extract collection and detection of pinoresinol diglucoside content in the methanol extract based on a HPLC method. The method is used for determination of gluconol diglucoside in Eucommia ulmoides.