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Application of eucommia ulmoides lignans extracts in preparation of medicine treating renal interstitial fibrosis

A technology of renal interstitial fibrosis and Eucommia lignan, which is applied in the direction of drug combination, pharmaceutical formula, plant raw materials, etc.

Active Publication Date: 2014-01-01
CHANGSHA DUXACT BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Application number: 200710035744.7 discloses the application of eucommia lignans and its extracts in anti-cardiovascular remodeling, and the new application of eucommia lignans extracts needs to be further studied

Method used

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  • Application of eucommia ulmoides lignans extracts in preparation of medicine treating renal interstitial fibrosis
  • Application of eucommia ulmoides lignans extracts in preparation of medicine treating renal interstitial fibrosis
  • Application of eucommia ulmoides lignans extracts in preparation of medicine treating renal interstitial fibrosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation of embodiment 1 eucommia lignan extract

[0030] Take 2Kg of bark of Eucommia ulmoides (with the outer skin removed), extract twice with 8L of 65% ethanol under reflux, each time for 1h, filter, combine the two extracts and concentrate to 800ml. Mix and load the sample with D101 macroporous resin (macroporous resin column volume 3L), wash with water for 3 times the column volume, continue to wash with 1% ammonia water for 3 times the column volume, and then wash with water until neutral. Elute with 20% ethanol for 2 column volumes and 45% ethanol for 2.5 column volumes. The 45% ethanol eluate was concentrated to 600ml. The eluate was concentrated and dried to a powder. Using pinoresinol diglucoside as a control, the lignan content of Eucommia ulmoides lignan extract was 85% detected by ultraviolet light, and the content of pinoresinol diglucoside detected by HPLC was 12.5%.

Embodiment 2

[0031] The preparation of embodiment 2 eucommia lignan extract

[0032] After the bark of Eucommia ulmoides is crushed, add 9L of 60% ethanol to reflux for extraction for 1 hour, filter, add 7L of 60% ethanol to the filter residue, extract for 1 hour, filter, combine the two extracts, concentrate until there is no alcohol smell, and obtain a concentrated solution of about 3L. The concentrated solution was passed through an AB-8 macroporous adsorption resin column, firstly eluted with 5L of water, then 7L of 60% ethanol, collected the 60% ethanol eluate, concentrated to 150ml, slowly added to 450ml of water, filtered, and concentrated to obtain Dry solid powder. Using pinoresinol diglucoside as a control, the lignan content of Eucommia ulmoides lignan extract was 51% by ultraviolet detection, and the content of pinoresinol diglucoside by HPLC was 3.5%.

Embodiment 3

[0034] The eucommia lignan extract obtained in Example 1 and Example 2 was used in the experimental study on the protection of renal interstitial fibrosis in Example 3.

[0035] 1. Experimental method

[0036] 1.1 Dose Design and Grouping

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Abstract

The invention relates to the field of purposes of extracts of traditional Chinese medicine, in particular to application of eucommia ulmoides lignans extracts in preparation of medicine treating renal interstitial fibrosis. Lignans accounts for 30-90% of the total weight of the eucommia ulmoides lignans extracts, and the effective constituent of pinoresinol diglucoside in the lignans accounts for 1%-15% of the total weight of the eucommia ulmoides lignans extracts.

Description

technical field [0001] The invention belongs to the field of use of extracts of traditional Chinese medicines, and in particular relates to a new use of eucommia lignan extracts. Background technique [0002] Renal fibrosis is a pathophysiological change, which is a gradual process in which the function of the kidney changes from healthy to damaged, then damaged, and finally lost. The result is progressive and irreversible damage to kidney function, which poses a huge threat to human health. Renal interstitial fibrosis is a common pathological feature of renal disease progressing to end-stage renal failure due to various reasons. A large number of clinical and pathological data show that chronic renal injury caused by various reasons is more closely related to the severity of renal interstitial fibrosis than glomerular lesions. Therefore, in order to slow down and reverse the progress of chronic kidney disease and improve renal function, it is of great significance to disc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/46A61P13/12
Inventor 姜德建严谨郭成贤李晓辉冯晗阳国平周宏灏欧阳冬生
Owner CHANGSHA DUXACT BIOTECH CO LTD
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