Apirulina containing spirulina bioactive peptide and spirulina proteinase, preparation method and application thereof
A technology of spirulina and active peptides, applied in the field of spirulina, can solve the problem of slow action
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Embodiment 1
[0015] (1) Carry out strain culture first, get 1 branch of Bacillus subtilis (the Bacillus subtilis existing commercial sale, the Bacillus subtilis used in the present invention buys from Institute of Microbiology, Chinese Academy of Sciences) inoculates on nutrient gravy agar medium, nutrient gravy The agar medium is mainly composed of the following raw materials: 10 grams of peptone, 3 grams of beef extract, 5 grams of agar, 5 grams of sodium chloride, plus sterile water to 1000 ml, and adjust the pH to 7. Seven strains were cultured at 35°C for 64 hours according to the conventional strain culture method, and then the cultured strains were combined to obtain the strain culture solution;
[0016] (2) Get 3 milliliters of the above-mentioned strain culture solution and add 35 kilograms of spirulina powder to the spirulina pulp with a water ratio of 1: 1.3 and stir evenly, ferment for 18 hours under the condition of 37° C., stir once every 4 hours during this period, let The a...
Embodiment 2
[0022] (1) is identical with the (1) step of embodiment 1;
[0023] (2) Get 5 milliliters of the above-mentioned strain culture solution and add 35 kilograms of spirulina powder to the spirulina pulp with a water ratio of 1: 1.3 and stir evenly, seal and ferment for 18 hours under the condition of 37° C., stir once every 4 hours during this period, Allow the algae pulp to ferment evenly;
[0024] All the other steps are the same as in Example 1.
[0025] The invention prepared by the above method has been tested by the Center for Disease Control and Prevention of Guangxi Zhuang Autonomous Region. In addition to the ingredients contained in conventional spirulina, it also contains 38.5% of spirulina active peptide and 11000 U / g of spirulina protease.
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