Spirulina containing active peptide, protease and protein kinase, and its processing method and application
A technology of spirulina and active peptides, applied in the field of spirulina, can solve problems such as slow action
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Embodiment 1
[0015] (1) Carry out strain culture first, get 1 branch of Bacillus subtilis (the Bacillus subtilis existing commercial sale, the Bacillus subtilis used in the present invention buys from Institute of Microbiology, Chinese Academy of Sciences) inoculates on nutrient gravy agar medium, nutrient gravy The agar medium is mainly composed of the following raw materials: 10 grams of peptone, 3 grams of beef extract, 5 grams of agar, 5 grams of sodium chloride, plus sterile water to 1000 ml, and adjust the pH to 7. Seven strains were cultured at 30-38°C for 24-48 hours according to the conventional strain culture method, and then the cultured strains were combined to obtain the strain culture solution;
[0016] (2) Take 15-30 ml of the above strain culture solution, add 35 kg of spirulina powder and water ratio of 1:1.3 to the spirulina slurry, stir evenly, and ferment at 37°C for 18-24 hours, during which every 4 hours Stir once to let the algae pulp ferment evenly;
[0017] (3) Th...
Embodiment 2
[0022] (1) is identical with the (1) step of embodiment 1;
[0023] (2) Take 5 ml of the above strain culture solution and add 35 kg of spirulina powder to water ratio of 1:1.3 into the spirulina slurry, stir evenly, seal and ferment at 37°C for 18-24 hours, stirring every 4 hours during the period Once, let the algae pulp ferment evenly;
[0024] All the other steps are the same as in Example 1.
[0025] The present invention prepared by the above-mentioned method is tested by Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention. Except containing the ingredients contained in conventional spirulina, it also contains 16.6% of spirulina active peptide, 210U / g of spirulina protease, and 35000FU / g of spirulina protein kinase. g.
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