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Method for determination of pinoresinol diglucoside in Eucommia ulmoides by ASE-HPLC method

A technology of pinoresinol diglucoside and methanol, which is applied in the field of chemical detection, can solve problems such as pressure failure and easy blockage, and achieve accurate measurement results

Inactive Publication Date: 2017-07-04
WUZHOU INST FOR FOOD & DRUG CONTROL
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Problems solved by technology

However, during the use of this method, the jelly in the Eucommia extract can easily block the filter of the static extraction valve, causing pressure failure

Method used

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  • Method for determination of pinoresinol diglucoside in Eucommia ulmoides by ASE-HPLC method
  • Method for determination of pinoresinol diglucoside in Eucommia ulmoides by ASE-HPLC method
  • Method for determination of pinoresinol diglucoside in Eucommia ulmoides by ASE-HPLC method

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Embodiment 1

[0029] Step 1: Take a sample of Eucommia ulmoides, cut it into pieces, crush it, pass through a No. 3 sieve, take about 1g, weigh it accurately, add diatomaceous earth in a ratio of 1:1 by weight, mix well, and put it in a 10ml extraction pool (sample loading Add an appropriate amount of diatomaceous earth to the bottom of the extraction pool before loading, and fill the extraction pool with diatomaceous earth after loading the sample).

[0030] Step 2: Put it in a Soxhlet extractor, add chloroform, heat and reflux for 6 hours, discard the chloroform extract, continue static extraction with methanol, recover the methanol extract into a 50ml measuring bottle, add methanol to the mark, Shake well, filter, and take the filtrate to obtain the methanol extract. The equipment is the ASE350 fast solvent extractor of DIONEX company in the United States;

[0031] Among them, the parameters for static extraction of methanol are as follows: extraction temperature is 100°C, extraction tim...

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Abstract

The invention discloses a method for determination of pinoresinol diglucoside in Eucommia ulmoides by an ASE-HPLC method and belongs to the field of chemical detection. The method utilizes an impurity removal process in the pharmacopeia, utilizes ASE to replace an extraction process, shortens pretreatment time and guarantees determination accuracy. A crushed sample of Eucommia ulmoides is extracted by an ASE extraction method. The ASE extraction method comprises extraction based on trichloromethane in a Soxhlet extractor, extraction based on methanol in a fast solvent extractor, methanol extract collection and detection of pinoresinol diglucoside content in the methanol extract based on a HPLC method. The method is used for determination of gluconol diglucoside in Eucommia ulmoides.

Description

technical field [0001] The invention belongs to the field of chemical detection, in particular to an ASE-HPLC method for measuring pinoresinol diglucoside in Eucommia ulmoides. Background technique [0002] "Chinese Pharmacopoeia" 2015 Edition Part One The method for preparing the test product is: take about 3g of this product, cut it into pieces, knead it into flocculents, take about 2g, weigh it accurately, put it in a Soxhlet extractor, and add an appropriate amount of chloroform , heated to reflux for 6 hours, discarded the chloroform liquid, evaporated the chloroform from the medicine dregs, put it in a Soxhlet extractor, added an appropriate amount of methanol, heated and refluxed for 6 hours, recovered an appropriate amount of methanol from the extract, and transferred it to a 10ml measuring bottle , add methanol to the mark, shake well, filter, take the continued filtrate, that is. The extraction time of this method is as long as 12 hours, and the operation is very ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 廖强韦日伟陈学松
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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