A forensic dna detection kit with auxiliary gender identification and its identification method

Abstract

The invention aims at solving the technical problem in the prior art that analysis data of the amplified chromosome locus cannot enter the China DNA database, provides a DNA detection kit for auxiliary gender identification for a legal medical expert, or a method for adding auxiliary gender identification to a conventional kit. The key point is that Y special-shaped amplification primer of a fluorescent mark identical to the mark internal standard SIZ mark primer is added on the conventional kit, so as to amplify a segment in SRY zone of the Y chromosome, such as chromosome locus M175 and SRY. Meanwhile, the invention further discloses an SIZ mark primer sequence used for amplifying chromosome locus SRY and M175. Through the improvement, the purpose of auxiliary gender identification can be achieved without adding other components to the kit, or influencing the types of the kit; meanwhile, the amplification segment of Y specificity has the peak at the same fluorescent of the internal standard, peaks of fluorescent marks of other colors are not influenced, the introduction of the data into the public security ministry DNA database is not influenced, thereby conforming to the actual situation of recording for the China public security ministry.

Description

technical field [0001] The invention belongs to the field of forensic DNA detection, and in particular relates to a method for detecting gender in an internal standard by marking a sex identification locus primer with an internal standard dye. Background technique [0002] Currently, forensic DNA detection uses multi-color (four-color, five-color, six-color) fluorescent compound amplification of 13-30 STR loci, and simultaneously detects the sex identification locus, the enamel protein gene Ameloginen, as listed in Table 1. Products and patents . [0003] The forensic detection DNA kit is divided into two parts, one part is called the pre-amplification kit, which includes primers for multiplex amplification (the primers are labeled with a different dye from the labeled internal standard) for PCR amplification; the other part is called the post-amplification kit. The kit, including molecular internal standard and allele standard, is used for capillary electrophoresis detecti...

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Problems solved by technology

[0010] Technical problem to be solved: Aiming at the technical problem that the analysis data of amplified chromosomal loci cannot be entered into the Chinese DNA database in the kits and identification methods in the prior art, the present invention provides a forensic DNA detection reagent with auxiliary gender identification kit, or add an auxiliary gender identification method to the forensic DNA detection kit, through such improvements, in the forensic DNA detection, the gender identification can be assisted without affecting the database storage

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