Micro-fluidic chip detection method based on magnetic bead uniform mixing

Abstract

The invention discloses a micro-fluidic chip detection method based on uniform mixing of magnetic beads. The method comprises the following steps of: step 1, presetting a solid reagent in a micro-fluidic chip; step 2, assembling the micro-fluidic chip; step 3, performing sample introduction; step 4, performing immune reaction; step 5, blowing away redundant liquid; and step 6, performing optical detection. According to the method of the invention, the step of cleaning with cleaning liquid is omitted, so that the operation steps are more simplified, the detection time is further shortened, and the detection efficiency is improved. The design structures related to corresponding external matched liquid path devices and corresponding cleaning flow channels of the chip are also simplified, the influence of the cleaning liquid on the compound after the immune reaction is effectively avoided, and the stability and the accuracy of a detection result are improved. According to the detection method, a uniform mixing reaction mode of upper and lower magnetic force interactive adsorption is adopted, so that the specific binding efficiency of an immune complex is improved, the immune complex is favorably separated from other components, the cleaning step of the complex is omitted; and the structure of external matched detection equipment is greatly simplified, and the production cost is reduced.

Description

technical field [0001] The invention relates to the field of microfluidic detection, in particular to a microfluidic chip detection method based on magnetic bead mixing. Background technique [0002] As a new type of solid-phase carrier reagent, superparamagnetic polymer microspheres have specific chemical groups on the surface that can bind to specific biomolecules, and the resulting immunomagnetic beads can specifically bind to corresponding target substances to form new When passing through a magnetic field, this complex can be retained and separated from other components. Immunomagnetic beads have superparamagnetic and nanoparticle properties in a magnetic field. Its superparamagnetism makes the solid-liquid separation operation easier and saves complicated traditional operations such as centrifugal filtration; and the nano-sized immunomagnetic beads have small particles, large specific surface area, large coupling capacity, and good suspension stability, which is condu...

AI Technical Summary

Problems solved by technology

[0004]1) Conventional magnetic bead technology uses conventional vessels to hold sample solutions and uses pipettes for cleaning and other operations, which is easy to introduce foreign pollutants

[0005]2) Conventional magnetic bead technology requires human manipulation of experimental steps, such as sampling, cleaning, etc., which is easy to introduce human error factors and cause experimental failure

[0006]3) The experimental platform of conventional magnetic bead technology is relatively large, requiring large equipment, difficult to integrate and miniaturize, and difficult to achieve portability

[0013] However, in the existing detection technology of magnetic bead technology combined with microfluidic chip, although the detection system has made great improvements in the above-mentioned advantageous directions, there are still There are following deficiencies: the cleaning step of the cleaning solution after the immune reaction makes the operation still cumbersome, and the detection time is relatively long; the implementation of the cleaning step also makes the design of the chip structure relatively complicated; and the residue of the cleaning solution will also cause detection. Inaccurate results

In addition, in the immune reaction, ultrasonic mixing is generally used, and an ultrasonic generator needs to be equipped accordingly, which makes the external supporting detection equipment more complicated, and the traditional ultrasonic mixing method is easy to damage the outer wall of the bottom of the reaction chamber, affecting the acquisition of subsequent fluorescence signals

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