35 results about "Magnetic Bead Technology" patented technology

The invention discloses a drawing forming die with blank pressing conducted by adopting an electronic control permanent magnet technique and a blank pressing method of the drawing forming die. The assembly of the drawing forming die with blank pressing conducted by adopting the electronic control permanent magnet technique is of a forward erection structure; the drawing forming die comprises an upper die base, a male die, a pressing plate, a female die, an electronic control permanent magnet sucking disc, a lower die base, a stripper bolt, a stopper screw and press sliding blocks; the male die is installed on the upper die base; the two press sliding blocks are installed on the upper die base symmetrically in the horizontal direction; the pressing plate is installed on the upper die base in a symmetric mode in the horizontal direction; the electronic control permanent magnet sucking disc is installed on the lower die base; the female die is located in a circular through hole in the center of the electronic control permanent magnet sucking disc; the female die is installed on the lower die base; the upper surface of the female die and the upper surface of the electronic control permanent magnet sucking disc are located on the same horizontal plane; the lower die base is provided with two vertical sliding channels which are symmetric in the horizontal direction; the two press sliding blocks are arranged on the two vertical sliding channels in a sleeving mode respectively and can slide along the vertical sliding channels freely in the vertical direction; the press sliding blocks are in clearance fit with the vertical sliding channels correspondingly; a through hole is formed in the center of the pressing plate, and the male die can penetrate the through hole and enter a female die cavity.

The invention discloses a reporter magnetic bead, which includes magnetic beads, nucleotides and enzymes with high catalytic activity linked. The invention also discloses a signal amplification magnetic bead technology system for nucleic acid detection based on CRISPR technology, and the invention also discloses a construction method and application of the reporter magnetic beads. The invention improves the sensitivity of the signal amplification magnetic bead technology system from the concentration level of aM (1000-10000 copies / mL) to the concentration level of zM (1-10 copies / mL) by introducing the reporter magnetic beads.

The invention discloses a polypeptide magnetic nanoparticle. The polypeptide magnetic nanoparticle comprises a specific targeting polypeptide and a magnetic nanoparticle, wherein the amino acid sequence of the specific targeting polypeptide is VRRDAPRFSMQGLDA-X, and the number of the C tail ends X of the specific targeting polypeptide is 5-20, preferably 5-15, and more preferably 9-12; preferably,amino acids in the X amino acid sequence are selected from one or more of C, G and N. The polypeptide nano magnetic bead technology can be used for CTC detection and molecular typing of various cancer types, including esophageal cancer, liver cancer, lung cancer, stomach cancer, breast cancer, colorectal cancer, cervical cancer and the like. The CTC molecular typing biomarker is prepared from PD-L1, HER2, ER, PR, AR, EGFR, CXCR4 and the like. The kit is beneficial to CTC detection and qualitative and semi-quantitative analysis of the expression level of the biomarker at the CTC level, so thattargeted individual targeted therapy or immunotherapy and other precise therapy are carried out on patients.

The present invention provides a nucleic acid aptamer of ovine pregnancy-associated glycoprotein (ovPAG). Derivatives derived from the sequences of the invention. The invention also provides the application of the nucleic acid aptamer. The nucleic acid aptamer of the present invention is screened by magnetic bead-SELEX technology, has high affinity with sheep PAG protein, and can be used for separation, enrichment and detection of PAG protein, and rapid diagnosis of early pregnancy in livestock.

The invention relates to the field of ophthalmology, in particular to a nucleic acid aptamer specifically binding to lipocalin-1 (LCN 1) and its application. The present invention obtains a set of high-affinity nucleic acid aptamers specifically binding to LCN 1 through magnetic bead SELEX technology screening, and further improves the nucleic acid aptamers by truncation and construction of highly stable mini-hairpin structures and other optimization strategies. performance. The nucleic acid aptamer of the present invention has broad application prospects, and can be used for the capture of LCN 1 in clinical samples, the detection of LCN 1 in vivo and in vitro, the development of LCN 1 therapeutic drugs in eye diseases, and the construction of targeted drug delivery systems. 1 The development of relevant detection technology, the development of clinical diagnostic methods and the research and development of nucleic acid drugs provide effective tools.

The invention discloses a capillary bioanalysis system, and an analytical method and applications thereof, and belongs to the field of medical equipment and biological detection technologies. The capillary bioanalysis system comprises a three-dimensional movement sample injecting platform, a temperature control-optical detection module, a magnetic field control module, a fluid control unit and a capillary array. The three-dimensional movement sample injecting platform and the fluid control unit are arranged on the two sides of the capillary array, and the capillary array is provided with the temperature control-optical detection module and the magnetic field control module. According to the capillary bioanalysis system, the droplet technology and the magnetic bead technology are combined, and bioanalysis processes such as loop-mediated isothermal amplification, fluorescence quantitative PCR analysis and immunochemiluminometry are integrated in capillaries. Advantages of the capillary bioanalysis system are that: volume is small, analysis speed is fast, detecting flux is large, and automation degree of operation is high. The capillary bioanalysis system is flexible in application, is suitable for analysis of single sample, batches of samples and on-site rapid detection, is capable of reducing acquisition cost and operation cost of equipment significantly, and possesses excellent economic benefits.

The invention discloses a microfluidic chip detection method based on magnetic bead technology. The method includes the steps of: 1, fluorescence labeling; 2, magnetic bead labeling; 3, coating and drying of a blocking solution; 4, immobilization of immunomagnetic beads; 5, immobilization of a fluorescence label; 6, microfluidic chip assembly; 7, sample adding preparation; 8, immunoreaction; 9, cleaning; 10, color development; and 11, data reading. The method provided by the invention selects highly sensitive time resolved fluorescent dye as the label for antibody labeling on magnetic beads and a fluorescent substance respectively, and utilizes the immunoreaction of the antibody pair for analysis and detection, and the performance of the prepared reagent can reach the level of equivalent chemiluminescent reagents. At the same time, the reagents adopt homogeneous liquid reaction to realize control of liquid flow. Ultrasonic mixing is adopted in the microfluidic chip chamber to avoid single-threaded chromatography flow reaction, therefore the reaction is fuller, and the reaction efficiency is higher.