5 results about "Magnetic Bead Technology" patented technology

Provided are reporting magnetic beads, the reporting magnetic beads comprising magnetic beads, nucleotides, and links to an enzyme having high catalytic activity. Further provided are a magnetic bead

This invention relates to the field of clinical laboratory technology and discloses an aptamer that specifically binds to AZGP1 and its applications. The invention uses magnetic bead SELEX technology to screen for the AZGP1-specific aptamer Seq7. Guided by Mfold secondary structure prediction, the core binding region Seq7T is truncated. Further, through site-directed mutagenesis, insertion, and substitution modifications, an optimized version, Seq7TM, containing specific motifs and capable of forming a compact mini hairpin structure, is obtained, further improving the performance of the nucleic acid aptamer. The aptamer of this invention can be widely used in the preparation of AZGP1 detection reagents, kits, or sensors; the preparation of AZGP1 capture / separation / purification formulations; the development of new methods for rapid early diagnosis of AMD; and the construction of AMD targeted drug delivery systems. It provides a novel and practical tool for early screening, early diagnosis, and early intervention of AMD, and has significant application value in the field of clinical laboratory testing.

This invention relates to a rapid nucleic acid extraction reagent for plant pathogens, its usage method, and a molecular detection kit. The invention provides a nucleic acid extraction reagent for plant pathogens and a lysis reagent containing this extraction reagent. On one hand, PVP-40 is added to the extraction reagent to improve cell permeability and enhance the nucleic acid extraction efficiency for plant pathogens, thereby improving detection efficiency and enabling rapid detection of plant pathogens. Simultaneously, the addition of SDS and CTAB to the lysis reagent synergistically disrupts the cell walls of plant pathogens, further enhancing the extraction efficiency of plant pathogen nucleic acids. Combined with magnetic bead technology, this enables batch extraction and detection analysis of plant pathogens. This invention provides reagents and methods for rapid and batch detection of plant pathogen nucleic acids, meeting the needs of different scenarios and suitable for rapid molecular detection of pathogens in field-exposed diseased plant tissues and pure cultured pathogens.

This invention relates to the field of biomedical technology, and particularly to a nucleic acid aptamer for the ABCA1 protein, its derivatives, and their applications. The nucleotides of the nucleic acid aptamer contain a core sequence as shown in any one of SEQ ID Nos. 3-5, and are capable of specifically binding to the ABCA1 protein. This invention provides a nucleic acid aptamer for the ABCA1 protein and its derivatives, the aptamer being able to effectively recognize the ABCA1 protein; it also provides a magnetic bead SELEX screening scheme for the ABCA1 protein nucleic acid aptamer, and the selected anti-Flag magnetic beads and NHS magnetic beads. Both anti-Flag and NHS magnetic beads can be well immobilized and can serve as good solid phases for screening and characterization.

The invention relates to a nucleic acid aptamer for specifically recognizing fibroblast growth factor 10 (FGF10) protein as well as a screening method and application of the nucleic acid aptamer. The nucleic acid aptamer is at least one of FAS-1, FAS-4, FAS-9, FAS-10 and FAS-12, and is obtained by screening through a magnetic bead-SELEX (systematic evolution of ligands by exponential enrichment) technology. The aptamer can be combined with FGF10 protein with high affinity, the equilibrium dissociation constant (Kd) of the aptamer is within the range of 5-20 nM, and the aptamer has good recognition specificity. By means of the excellent performance, the nucleic acid aptamer has wide application prospects in construction of an efficient detection method of the FGF10 protein and research and development of an FGF10 antagonist.