ssDNA aptamer capable of specifically recognizing N-acetylneuraminic acid and application thereof

A technology of acetylneuraminic acid and aptamer, which is applied in the direction of recombinant DNA technology, DNA/RNA fragments, fluorescence/phosphorescence, etc., can solve the problems of limiting the application of immunoassay methods, limited types of enzyme-labeled antibodies, production and application restrictions, etc. , to achieve the effect of high affinity, easy modification and labeling, and low cost
CN113151283AActive Publication Date: 2021-07-23JIANGNAN UNIV
2 Cites 2 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2021-07-23

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to an ssDNA aptamer capable of specifically recognizing N-acetylneuraminic acid and application thereof. According to the invention, 4 ssDNA aptamer sequences capable of being highly specifically combined with Neu5Ac are obtained through screening of a magnetic bead-SELEX (systematic evolution of ligands by exponential enrichment) technology, and sequence ap1 has the highest affinity and specificity; and moreover, sequence ap1 is stable in structure. On the basis of sequence ap1, the sequence is truncated according to a secondary structure and a molecular simulation docking result to obtain one aptamer ap2 with the sequence length of 21nt, and the affinity is improved compared with that before truncation. The ssDNA aptamer disclosed by the invention is relatively high in binding specificity and affinity with Neu5Ac, and is high in sensitivity when being applied to Neu5Ac detection.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the fields of biochemistry and molecular biology, analytical chemistry and combinatorial chemistry, in particular to ssDNA aptamers specifically recognizing N-acetylneuraminic acid and applications thereof. Background technique

[0002] N-acetylneuraminic acid (Neu5Ac) is the most studied and intensively studied sialic acid, and it is a precursor for the synthesis of other classes of sialic acid. As an important part of glycoconjugates, sialic acid can promote mutual repulsion between cells, stabilize cell membranes, increase the viscosity of coupled glycoproteins, etc. due to the negative charge it carries. Sialic acid is also an important biological information transmission molecule, such as participating in the recognition of host and pathogen and the reaction between cells, and participating in the phagocytosis of macrophages on aging red blood cells. It has been previously demonstrated that, on the one hand, the abnormal ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More