Polypeptide magnetic nanoparticle and preparation method and application thereof

Abstract

The invention discloses a polypeptide magnetic nanoparticle. The polypeptide magnetic nanoparticle comprises a specific targeting polypeptide and a magnetic nanoparticle, wherein the amino acid sequence of the specific targeting polypeptide is VRRDAPRFSMQGLDA-X, and the number of the C tail ends X of the specific targeting polypeptide is 5-20, preferably 5-15, and more preferably 9-12; preferably,amino acids in the X amino acid sequence are selected from one or more of C, G and N. The polypeptide nano magnetic bead technology can be used for CTC detection and molecular typing of various cancer types, including esophageal cancer, liver cancer, lung cancer, stomach cancer, breast cancer, colorectal cancer, cervical cancer and the like. The CTC molecular typing biomarker is prepared from PD-L1, HER2, ER, PR, AR, EGFR, CXCR4 and the like. The kit is beneficial to CTC detection and qualitative and semi-quantitative analysis of the expression level of the biomarker at the CTC level, so thattargeted individual targeted therapy or immunotherapy and other precise therapy are carried out on patients.

Description

technical field [0001] The invention belongs to the field of medical detection, and in particular relates to a polypeptide magnetic nano particle, its preparation method and application. Background technique [0002] Cancer has become a kind of disease that seriously endangers human health and life, and more than 8 million people die of cancer every year in the world. In clinical practice, the routine tumor detection methods are mostly imaging and tissue biopsy, limited by the resolution of imaging, it is difficult to find tumors smaller than 5mm. However, tissue biopsy is difficult to achieve multiple sampling, and brings pain and risk to patients. Circulating tumor cells (CTCs) that fall off from the primary tumor and enter the blood circulation carry almost all the genetic and protein information of the in situ tumor tissue. It provides a basis for predicting curative effect, evaluating prognosis and monitoring recurrence. [0003] Tumors are highly heterogeneous. Even...

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Problems solved by technology

[0003] Tumors are highly heterogeneous. Even tumors with the same histology and morphology have different molecular biological changes. Different biological changes have different biological behaviors and treatment sensitivities. The current clinical staging Traditional pathological typing, such as grading and grading, has limited predictive power for tumors

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