Microfluidic chip detection method based on magnetic bead technology

Abstract

The invention discloses a microfluidic chip detection method based on magnetic bead technology. The method includes the steps of: 1, fluorescence labeling; 2, magnetic bead labeling; 3, coating and drying of a blocking solution; 4, immobilization of immunomagnetic beads; 5, immobilization of a fluorescence label; 6, microfluidic chip assembly; 7, sample adding preparation; 8, immunoreaction; 9, cleaning; 10, color development; and 11, data reading. The method provided by the invention selects highly sensitive time resolved fluorescent dye as the label for antibody labeling on magnetic beads and a fluorescent substance respectively, and utilizes the immunoreaction of the antibody pair for analysis and detection, and the performance of the prepared reagent can reach the level of equivalent chemiluminescent reagents. At the same time, the reagents adopt homogeneous liquid reaction to realize control of liquid flow. Ultrasonic mixing is adopted in the microfluidic chip chamber to avoid single-threaded chromatography flow reaction, therefore the reaction is fuller, and the reaction efficiency is higher.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a microfluidic chip detection method based on magnetic bead technology. Background technique [0002] As a new type of solid-phase carrier reagent, superparamagnetic polymer microspheres have specific chemical groups on the surface that can bind to specific biomolecules, and the resulting immunomagnetic beads can specifically bind to corresponding target substances to form new When passing through a magnetic field, this complex can be retained and separated from other components. Immunomagnetic beads have superparamagnetic and nanoparticle properties in a magnetic field. Its superparamagnetism makes the solid-liquid separation operation easier and saves complicated traditional operations such as centrifugal filtration; and the nano-sized immunomagnetic beads have small particles, large specific surface area, large coupling capacity, and good suspension stability, which is ...

AI Technical Summary

Problems solved by technology

After the immune reaction, the immunomagnetic beads are immobilized by applying an external magnetic field, and the redundant components are completely removed with the cleaning solution, and then the chromogenic solution is added for reading, which solves the problem of difficult or incomplete cleaning inside the chip

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