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Salmonella fluorescence quantitative determination immunochromatography kit

A fluorescence quantitative detection, Salmonella technology, applied in the field of medical testing, can solve the problems of large background interference, low sensitivity, single color, etc., achieve uniform reaction and signal release, improve detection efficiency, and fast detection effect.

Inactive Publication Date: 2015-02-25
GUANGZHOU WONDFO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this method, the stability of the marker is poor, the color is single, the sensitivity is low, and the background interference of the matrix is ​​large, and it can only be detected qualitatively, and cannot be accurately quantified.

Method used

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  • Salmonella fluorescence quantitative determination immunochromatography kit
  • Salmonella fluorescence quantitative determination immunochromatography kit
  • Salmonella fluorescence quantitative determination immunochromatography kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Fluorescence Immunochromatography Kit for Detecting Salmonella in Pork Samples

[0030] In this embodiment, the immunochromatographic kit for fluorescent quantitative detection of Salmonella includes a test strip, an immunomagnetic bead solution and a fluorescent labeling solution.

[0031] Among them, the test strip consists of the sample pad, the cellulose coating film including the detection area (T area) and the quality control area (C area), and the absorbent paper are pasted on the bottom plate in sequence according to the conventional method of the test strip.

[0032] In this embodiment, the detection zone T line of the coated membrane is coated with Salmonella monoclonal antibody or polyclonal antibody at a concentration of 0.3 mg / ml and an amount of 90 μl / 27-35 cm. The C line of the quality control area of ​​the coated membrane is coated with a goat anti-rabbit IgG antibody with a concentration of 0.3mg / ml and an amount of 90μl / 27-35cm, which is u...

Embodiment 2

[0043] Example 2: Preparation of Fluorescence Immunochromatography Kit for Detecting Salmonella in Egg Samples

[0044] In this embodiment, the immunochromatographic kit for fluorescent quantitative detection of Salmonella includes a test strip, an immunomagnetic bead solution and a fluorescent labeling solution.

[0045] Among them, the test strip consists of the sample pad, the cellulose coating film including the detection area (T area) and the quality control area (C area), and the absorbent paper are pasted on the bottom plate in sequence according to the conventional method of the test strip.

[0046] In this embodiment, the detection zone T line of the coated membrane is coated with Salmonella monoclonal antibody or polyclonal antibody at a concentration of 2.5 mg / ml and an amount of 90 μl / 27-35 cm. The C line of the quality control area of ​​the coated membrane is coated with a goat anti-rabbit IgG antibody with a concentration of 2.5mg / ml and an amount of 90μl / 27-35...

Embodiment 3

[0060] Example 3: Preparation of Fluorescent Immunochromatography Kit for Detecting Salmonella in Milk Samples

[0061] In this embodiment, the immunochromatographic kit for fluorescent quantitative detection of Salmonella includes a test strip, an immunomagnetic bead solution and a fluorescent labeling solution.

[0062] Among them, the test strip consists of the sample pad, the cellulose coating film including the detection area (T area) and the quality control area (C area), and the absorbent paper are pasted on the bottom plate in sequence according to the conventional method of the test strip.

[0063] In this embodiment, the T line in the detection area of ​​the coated membrane is coated with Salmonella monoclonal antibody or polyclonal antibody with a concentration of 3.5 mg / ml and an amount of 90 μl / 27-35 cm. The C line of the quality control area of ​​the coated membrane is coated with a goat anti-rabbit IgG antibody with a concentration of 3.5mg / ml and an amount of...

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Abstract

The invention discloses a Salmonella fluorescence quantitative determination immunochromatography kit which includes a test paper strip, immunomagnetic beads and a fluorescent labeling solution, the test paper strip is prepared by sequential lapped paste of a sample pad, a nitrocellulose coated film and absorbent paper on a polyethylene plastic bottom plate;a testing area of the nitrocellulose coated film is coated with Salmonella monoclonal antibody or polyclonal antibody, a quality control area is coated with anti-rabbit IgG antibody; the immunomagnetic bead surface is coupled with the Salmonella monoclonal antibody; and the fluorescent labeling solution contains fluorescent latex-marked Salmonella monoclonal antibody or polyclonal antibody and fluorescent latex-marked anti-rabbit IgG antibody. The Salmonella fluorescence quantitative determination immunochromatography kit combines magnetic bead technology and immunochromatographic fluorescence quantitative detection technology, after enrichment and concentration of Salmonella in a sample for detection by use of the immunomagnetic beads, quantitative determination is performed, and compared with a simple pure fluorescence quantitative chromatography detection kit, the Salmonella fluorescence quantitative determination immunochromatography kit greatly improves the detection efficiency.

Description

[0001] technical field [0002] The invention belongs to the field of medical inspection, in particular to a fluorescent quantitative detection Salmonella immunochromatography kit. Background technique [0003] Food is the material basis for the survival and development of human society, and with the frequent occurrence of food safety accidents causing extremely serious personal and property safety, food safety has become one of the focus issues of all walks of life in today's society. [0004] Salmonella (Salmonella) is a zoonotic foodborne pathogen. Salmonella is widely distributed in nature and has a wide range of hosts in humans and animals. Salmonella has a high probability of contaminating meat food, such as pigs, cattle, sheep, cats, and dogs in livestock, and chicken, duck, and goose in poultry. Foods of animal origin are an important part of the human diet. Bacterial food poisoning is the most common food poisoning of animal origin, and food poisoning caused by S...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N21/64
CPCG01N33/56916G01N2333/255
Inventor 王继华唐时幸刘晓云
Owner GUANGZHOU WONDFO BIOTECH
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