58 results about "Foodborne pathogen" patented technology

The invention relates to an extraction method and application of hippophae rhamnoides leaf polyphenol, and belongs to the technical field of food functional component extraction. According to the method, the hippophae rhamnoides leaf polyphenol is extracted by innovatively adopting a wall-breaking-step extraction system combining enzymatic pretreatment with ultrasonic induction, and the method is specifically characterized in that hippophae rhamnoides leaf powder is used as a substrate, and normal-temperature enzymolysis wall-breaking treatment is performed. Furthermore, a sound field is combined to promote non-thermal dissociation of secondary metabolites in the hippophae rhamnoides leaf powder, and a continuous process of'normal-temperature enzymolysis of cellulase-ultrasonic extraction 'is constructed. Then, adding an ethanol solution, and placing in a water bath for reaction extraction; and centrifugally freeze-drying to obtain the crude polyphenol of the sea buckthorn leaves. An antibacterial experiment shows that the hippophae rhamnoides leaf polyphenol crude extract has a remarkable antibacterial effect on food-borne pathogenic bacteria such as vibrio parahaemolyticus and aeromonas hydrophila, the technical scheme effectively keeps the biological activity of polyphenol, meanwhile, the solvent consumption and the oxidative degradation rate of heat-sensitive components are lower than those of a traditional process, and the method is suitable for development of natural preservatives and functional foods.

This invention discloses a broad-spectrum Staphylococcus aureus bacteriophage, SapYZUbeta, and its applications. This bacteriophage has been deposited at the China Center for Type Culture Collection (CCTCC) on May 16, 2022, with accession number CCTCC NO: M 2022629. Identification revealed that the full-length gene of this bacteriophage is 17,790 bp, and it does not contain tRNAs or genes associated with lysogenicity, virulence, or antibiotic resistance. This genetic background confirms the safety of this bacteriophage for use against foodborne pathogens and for bacteriophage therapy. Furthermore, the bacteriophage exhibits a high titer of 1.9 × 10⁻⁶. 9 The phage, with a concentration of PFU / mL, exhibits stable activity within a pH range of 3–12 and a temperature range of -80–60°C, and can be applied to various matrices. The phage provided by this invention can lyse multidrug-resistant wild-type strains isolated from different regions, including MRSA and MSSA, and is characterized by a broad lysis spectrum and high lysis efficiency.

The present invention discloses a Ni / Co(HHTP)MOF electrode material, a preparation method thereof, and an application thereof in the preparation of an electrochemical aptamer sensor for detecting Escherichia coli O157:H7, belonging to the technical field of harmful microorganism detection. The electrochemical aptamer sensor is prepared by modifying a rod-shaped Ni / Co(HHTP)MOF material onto a glassy carbon electrode. The electrochemical aptamer sensor of the present invention can efficiently and specifically capture Escherichia coli O157:H7 and can be used to quickly and sensitively detect Escherichia coli O157:H7 in food. Compared with traditional bacterial detection methods, the method of the present invention has good selectivity, storage stability and reproducibility, a low detection limit, and a short detection time, providing a new idea for the detection of foodborne pathogens and having good application prospects.

The present invention provides a strain of Bifidobacterium breve BX-18 and its application, and belongs to the field of biotechnology. The deposit number of Bifidobacterium breve (Bifidobacterium breve) BX-18 provided by the present invention is CGMCCNo.28446. The Bifidobacterium breve BX-18 can synthesize active substances such as amino acids and metabolites thereof, benzene derivatives, flavonoids, and its metabolites have broad-spectrum antibacterial activity and have good antibacterial effect on foodborne pathogens or spoilage bacteria. The present invention provides a new idea for the in-depth study and utilization of probiotics with functional active substances, which has huge research and application value.

A method for rapidly detecting and identifying food-borne pathogens by using a two-dimensional / zero-dimensional MXene / GQDs Schottky heterojunction fluorescence sensor array based on machine learning comprises the following steps: synthesizing three nitrogen-doped graphene quantum dots through a hydrothermal method, determining the optimal excitation wavelength, concentration, reaction temperature and pH value of the nitrogen-doped graphene quantum dots, and further determining the fluorescence intensity of the nitrogen-doped graphene quantum dots under the optimal condition; constructing a fluorescence sensor array, mixing the three graphene quantum dots with target heavy metal ions, incubating, then measuring fluorescence intensity to obtain fluorescence response data, and normalizing the data to obtain the fluorescence response data. And analyzing by using machine learning methods of a decision tree, a support vector machine, K nearest neighbor, linear discriminant analysis, naive Bayes and a neural network. The prepared fluorescence sensor array can rapidly identify heavy metal ions, is excellent in accuracy, sensitivity and stability, provides an economical and efficient solution for heavy metal ion detection, and has remarkable application potential in environmental monitoring.

The invention relates to the technical field of aquatic product quality safety detection, in particular to an aquatic product quality safety detection device for rapidly screening food-borne pathogenic bacteria. Comprising a workbench, a support, a suction head bin, a sample frame, a detection module, a suction head recovery box, a movement module and a pipetting module, the support is fixedly arranged at the top of the workbench, and the suction head bin, the sample frame, the detection module and the suction head recovery box are arranged between the workbench and the support and are sequentially arranged from left to right; the movement module is fixedly arranged at the inner top of the bracket, and the pipetting module is movably arranged below the movement module; an integrated, processized and automatic detection platform is constructed, the complete process from suction head installation, sample / reagent suction, liquid transfer and distribution to final suction head discarding is automatically completed, instability and fatigability of manual operation are avoided, and the overall detection efficiency and consistency are remarkably improved.

The invention provides an antibacterial peptide for inhibiting food-borne pathogens as well as an acquisition method and application of the antibacterial peptide, and relates to the technical field of bioactive peptides and biomedical engineering. The amino acid sequence of the antibacterial peptide is as follows: Gly-Leu-Thr-Leu-Lys-His-Leu-Lys-Lys-Leu-Phe, and the antibacterial peptide can be used for effectively inhibiting the growth of food-borne pathogens such as staphylococcus aureus, salmonella, escherichia coli, pseudomonas aeruginosa, listeria monocytogenes and the like. The defects in the prior art are overcome, the antibacterial peptide with antibacterial activity is found and identified through a screening method combining a gastrointestinal digestive enzyme-peptide omics technology and an experiment, and a new natural antibacterial agent is provided for food, medicine and other related fields.

This invention discloses a strain of Salmonella choleraesuis phage SCP512 and its applications, belonging to the field of microbial technology. Salmonella choleraesuis phage SCP512 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 47021, dated February 4, 2026. It belongs to the family Myocaudae within the class Caudata. This phage SCP512 possesses multivalent lytic characteristics, excellent biological properties and environmental adaptability, and can disrupt pathogen biofilm formation. It addresses the technical problems of existing phages, such as limited lytic spectrum, insufficient coverage of common foodborne pathogens, weak biofilm removal ability, and limited application potential in practical food matrices.

This invention discloses a lactic acid bacteria bacteriocin, its preparation method, and its applications, belonging to the field of microbial technology. The invention extracts a bacteriocin from *Lactobacillus pentosus* with accession number CCTCC NO: M 2022431. This bacteriocin exhibits thermal stability, can withstand low temperatures, and maintains its activity over a wide pH range. Simultaneously, this bacteriocin can inhibit the growth and proliferation of the foodborne pathogen *Listeria monocytogenes*. Because it originates from edible Sichuan pickled vegetables, this bacteriocin is harmless to humans and can provide a new research direction for the prevention or treatment of diseases caused by foodborne pathogens, thus showing promising application prospects.

The invention relates to the field of microorganisms and fermentation engineering, in particular to acid-producing plant lactobacillus and application thereof. Specifically, the bacterial strain is named as the lactobacillus plantarum BLSCX-2, and the bacterial strain is named as the lactobacillus plantarum BLSCX-2. Tests prove that the yield of lactic acid and phenyllactic acid is obviously higher than that of reported strains, so that the strain is suitable for efficient fermentation food production. Meanwhile, the plant lactobacillus BLSCX-2 disclosed by the invention has a broad-spectrum bacteriostatic effect on various food-borne pathogenic bacteria and putrefying bacteria, and can be used for biological preservation or development of medical bacteriostatic agents. Besides, the strain can still keep activity under the conditions of low pH, high bile salt, low temperature and high salt, and the application range of the strain in a complex environment is widened, so that the strain has good practical application value.

The application discloses five kinds of high-throughput detection primer groups, kits and application of LAMP method for foodborne pathogenic bacteria, and belongs to the technical field of foodborne pathogenic bacteria detection. The primer groups correspond to five kinds of bacteria, including Salmonella, Vibrio parahaemolyticus, Listeria monocytogenes, Escherichia coli O157:H7 and Staphylococcus aureus, and the nucleotide sequences of the primers are SEQ ID No. 1-30. The complete process detection can be completed at 62 DEG C constant temperature for 45 minutes, the result does not need complex instruments, the positive gray and the negative purple are judged by naked eyes, and the operation is simple. The detection sensitivity reaches 1-75 copies / reaction, the artificially contaminated aquatic product sample does not need to be subjected to bacterial enrichment, is subjected to simple lysis and enrichment, has strong specificity and no cross reaction. The anti-interference ability is outstanding, the complex matrix of aquatic products is adapted, the detection coincidence rate is high, the cost is low, only a conventional constant temperature device is needed, and the application is suitable for multiple scenes, such as detection institutions, food enterprises and on-site law enforcement on-site detection of primary food, agriculture, disease control, customs and the like, and provides an efficient and economical technical means for aquatic product safety control.

This invention discloses a quadruple Taqman qPCR reagent for detecting foodborne pathogens in aquatic products. The foodborne pathogens are Vibrio parahaemolyticus, Vibrio vulnificus, Shigella, and Staphylococcus aureus. The reagent includes specific primers and Taqman probes corresponding to the four foodborne pathogens. This invention provides a quadruple Taqman qPCR reagent with high efficiency, accuracy, sensitivity, and speed, offering an accurate, rapid, efficient, and economical method for detecting the above four foodborne pathogens in the quality and safety testing of aquatic products and their processed products. This is of great significance for ensuring the safety of aquatic products for consumption in my country.

The application relates to the technical field of detection, in particular to a general foodborne pathogenic bacteria detection method suitable for a long-distance sailing environment. UiO-66 is used to enrich methylene blue, and is combined with target bacteria aptamer to obtain UiO-66 / MB / Aptamer which simultaneously serves as a signal amplification and recognition probe. A membrane separation method is used to filter out the probes not combined with the bacteria. The electrochemical signal of methylene blue is detected by using a differential pulse voltammetry method, a corresponding linear relationship is constructed, and the concentration of the bacteria is quantitatively detected. The application not only guarantees the sensitivity, detection time, portability and other characteristics of the detection of foodborne pathogenic bacteria, but also provides a general detection method for detecting various target bacteria. Through the reproducibility and stability test under the high-temperature, high-humidity and high-salt environment, it is proved that the application can realize the rapid, sensitive and stable detection of foodborne pathogenic bacteria in a complex long-distance sailing environment.

This invention relates to an aptamer of *Cronobacter sakazakii* and a method for preparing a fluorescence-quenched biosensor thereof, belonging to the field of biosensor technology. The DNA sequence of the *Cronobacter sakazakii* aptamer is shown in SEQ ID No:1. Experiments show that the cross-reactivity of this aptamer with common foodborne pathogens such as *Staphylococcus aureus* and *Listeria monocytogenes* is less than 25%. The construction process of the fluorescence-quenched biosensor based on the *Cronobacter sakazakii* aptamer includes the mixing of activating agents, the preparation of fluorescent probes and quenching probes, and the mixing of fluorescent probes and quenching probes in a volume ratio to obtain a sensitive and specific fluorescence-quenched biosensor. The fluorescence-quenched biosensor of this invention exhibits high linearity and low detection limits; simultaneously, the screening method is simple and convenient, requiring only the use of an existing Autodoc server for rapid screening.

The invention discloses a primer probe combination based on food-borne pathogenic bacterium detection and a kit thereof. The detection sensitivity is high, the specificity is good, multiple food-borne pathogenic bacteria can be detected at the same time at a time, the quantitative potential is achieved, a basis is provided for food-borne disease diagnosis, and the method can play an important role in the fields of food safety, quality control and food-borne pathogen disease prevention and diagnosis.

An example system includes a light intensity measuring apparatus couplable to a food processing apparatus and a computing system. The light intensity measuring apparatus includes a chamber configured to receive a water sample from the food processing apparatus, a light source, a detector configured to detect light that has passed through the water sample and measure multiple times intensities of wavelengths of the light to obtain multiple sets of measured intensities of wavelengths, and a communication module configured to provide the multiple sets of measured intensities of wavelengths. The computing system may receive the multiple sets of measured intensities, process the multiple sets to obtain a set of values, apply a first set of decision trees to the set of values to obtain a first result indicating a positive or negative foodborne pathogen detection, generate a notification indicating either the positive of negative detection, and provide the notification.

This invention relates to a composite material and its preparation method for adsorbing and catalytically degrading foodborne pathogens, belonging to the field of composite material technology. The composite material for adsorbing and catalytically degrading foodborne pathogens comprises a porous nanocarrier, a photocatalytically active component loaded on the porous nanocarrier, and nano-silver modified on the surface of the photocatalytically active component; wherein the porous nanocarrier is graphene oxide; the photocatalytically active component is titanium dioxide and zinc oxide in a mass ratio of 2:1 to 1:2; and the mass ratio of the photocatalytically active component to the porous nanocarrier is 1:5 to 1:1. The composite material of this invention possesses both high-efficiency, broad-spectrum antibacterial properties and long-term stability, and can be widely used in the food antibacterial industry.

This invention relates to the field of food packaging materials technology, and provides a silver-bipyridine column[5]arene coordination polymer / chitosan composite film, its preparation method, and its application. The silver-bipyridine column[5]arene coordination polymer / chitosan composite film of this invention achieves long-term inhibition of common foodborne pathogens through a dual mechanism of direct antibacterial action by silver ions and peroxidase-like catalysis enhanced by column[5]arene, combined with a stable coordination polymer network constructed from bipyridine bifunctionalized column[5]arene. It can block harmful ultraviolet rays to prevent food photo-oxidation and maintain high light transmittance for easy observation. Relying on its long-term antibacterial effect and ultraviolet blocking function, it can extend the shelf life of food, maintain its freshness and nutritional value. Using biodegradable chitosan as a matrix, it meets the development requirements of green packaging.

The invention discloses fruit and vegetable washing salt and a preparation method thereof. The fruit and vegetable washing salt comprises the following components in parts by weight: 40-60 parts of refined salt; 15-30 parts by weight of baking soda; 25-40 parts by weight of citric acid; and 0.5-2 parts by weight of a nano metal antibacterial agent. Through a ternary synergistic system of nano-silver, baking soda and citric acid, a composite effect of physical adsorption, chemical decomposition and biological sterilization is generated, through detection, the removal rate of organophosphorus pesticides is increased to 92% or above, and the killing rate of common food-borne pathogenic bacteria such as escherichia coli reaches 99.9% standard; by means of the innovative double-process route design, the product can meet the differentiated requirements of powder (high dissolving speed) and tablets (accurate using metering) at the same time, a user can select the cleaning mode according to the types of fruits and vegetables, the effervescence phenomenon can be generated, and the fruit and vegetable cleaning effect is improved.

The invention provides a rapid identification method for food-borne pathogenic bacteria and spores based on Raman spectrum and machine learning. The method comprises the following steps: 1, carrying out Raman spectrum data acquisition and pretreatment to obtain a Raman spectrum data set; step 2, constructing a classification model based on SPA-RF: performing feature extraction on the Raman spectrum data by adopting a continuous projection algorithm, and classifying the data after feature extraction by adopting a random forest algorithm; step 3, training the SPA-RF-based classification model by using a Raman spectrum data set to obtain a model with optimal performance, and performing interpretability analysis on the model with the optimal performance by using an SHAP method; 4, unknown Raman spectrum data samples to be classified are preprocessed through the preprocessing method in the step 1, classification is conducted through the model with the optimal performance, a classification result is obtained, and the identification system based on the B / S architecture is further provided. According to the invention, automatic processing and classification prediction of Raman spectrum data are realized, and the efficiency and accuracy of microbiological detection are improved.

The utility model discloses a device for detecting foodborne pathogenic microorganisms in fresh-cut fruits and vegetables, which comprises a sample liquid chamber, an enrichment chamber, a filter membrane plate, a plurality of microporous filter membranes and a plurality of culture substrates, culture media are arranged in the plurality of culture substrates, a support plate is fixedly connected to the upper side of the interior of the enrichment chamber, a placing groove is arranged on the support plate, and the sample liquid chamber is arranged in the placing groove. The filter membrane plate is placed in the placing groove, a plurality of circular grooves are formed in the filter membrane plate, a plurality of microporous filter membranes are mounted on the bottom sides in the circular grooves respectively, a cover plate is hinged to the left side of the top end of the enrichment chamber, the sample liquid chamber is mounted on the cover plate, the left side in the sample liquid chamber is communicated with a first connecting pipe, and a first valve is mounted on the first connecting pipe; the fresh-cut fruit and vegetable filtrate or the buffer cleaning sample liquid thereof is prevented from being conveyed to a plurality of microporous filter membranes by using a pump machine, damage to part of food-borne pathogenic microorganisms in the fresh-cut fruit and vegetable filtrate or the buffer cleaning sample liquid thereof is avoided, and influence on the accuracy of a detection result is avoided.

This invention belongs to the field of food packaging technology, specifically relating to a biomimetic breathable preservation film, its preparation method, and its application. The invention uses polylactic acid as a base material, adding two inorganic modified compounds: amino acid-functionalized cobalt-aluminum-gallium ternary layered bimetallic hydroxide nanosheets and an iron-copper bimetallic organic framework material loaded with silver nanoparticles. Plasticizers and surfactants are also added, and the film is prepared through solution blending, low-temperature ultrasonic dispersion, and casting. This preservation film possesses biomimetic breathable functionality, with oxygen and carbon dioxide permeability within the suitable range for modified atmosphere preservation of fruits and vegetables. The carbon dioxide to oxygen permeability ratio is also reasonable, exhibiting efficient ethylene removal capabilities and broad-spectrum antibacterial activity against common foodborne pathogens. Using this preservation film for fruit and vegetable packaging can significantly extend the shelf life of fresh agricultural products such as grapes and blueberries. The preparation process of this invention is simple and has broad application prospects in the field of active food packaging.

A loop-mediated isothermal amplification (LAMP) platform is disclosed to detect pathogens by integrating LAMP, a rapid tissue lysis method, and an embedded label-free sensor in a reaction / detection chamber, in which the LAMP primers are immobilized on the nanopore thin film. Amplified LAMP products are attached to the sensor surface via the immobilized primers and produce pronounced transducing signals that can be directly measured by an optical spectrometer. The integrated sensing platform requires neither the labeling of LAMP products nor the laborious DNA purification step. This label-free sensing technology enables ultrasensitive, specific, rapid, and cost-effective point-of-care diagnostics of plant, animal, human, and foodborne pathogens.

This invention provides an antimicrobial peptide that inhibits foodborne pathogens, its acquisition method, and its applications, relating to the fields of bioactive peptides and biomedical engineering technology. The amino acid sequence of the antimicrobial peptide is: Gly-Leu-Thr-Leu-Lys-His-Leu-Lys-Lys-Leu-Ile-Phe. This antimicrobial peptide can effectively inhibit the growth of foodborne pathogens such as Staphylococcus aureus, Salmonella, Escherichia coli, Pseudomonas aeruginosa, and Listeria monocytogenes. This invention overcomes the shortcomings of existing technologies by using a screening method combining gastrointestinal digestive enzyme-peptidomics technology and experiments to discover and identify antimicrobial peptides with antimicrobial activity, providing new natural antimicrobial agents for the food, pharmaceutical, and other related fields.

This invention discloses a laser-irradiated *Lactobacillus amylovorus* and its application in controlling foodborne pathogens, belonging to the field of microbial technology. The *Lactobacillus amylovorus* LY2332 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37009. This invention induces metabolic remodeling in *Lactobacillus amylovorus* through laser irradiation, effectively enhancing its environmental adaptability and the antibacterial activity of its cell-free supernatant, and demonstrating good application results in the preservation of chilled meat. This invention not only clarifies that laser irradiation can enhance the antibacterial activity of cell-free supernatant of lactic acid bacteria, but also provides a new strategy for developing natural food control agents and lays a theoretical foundation for the industrial application of lactic acid bacteria in food preservation.

This invention relates to the field of gene detection, specifically to a single-tube multiplex detection sequence combination, kit, detection method, and application for foodborne pathogens based on asymmetric ratios and melting curves. This invention provides a sequence combination for detecting foodborne pathogens, comprising a forward primer, a reverse primer, a specific probe, and a reverse partially complementary melting curve probe. This invention also provides a kit for detecting foodborne pathogens, which, through adjustment of the asymmetric ratios, solves the problems of systemic competitive inhibition and cross-matching in complex genetic backgrounds, constructing a single-tube detection method for five types of diarrheal Escherichia coli, Salmonella, Listeria monocytogenes, and Staphylococcus aureus. This method does not require additional complex hardware channels or expensive detection equipment, and has the advantages of high throughput, strong specificity, and low instrument cost, making it suitable for application and promotion in low-resource grassroots institutions, customs, and front-line food safety supervision.

The utility model discloses a multi-specification lateral flow test strip for detecting food-borne pathogen toxins, which comprises a test strip shell, two connecting blocks are fixedly connected to the inner side wall of the test strip shell, and a sealing plate is fixedly connected to the side surfaces, close to each other, of the two connecting blocks. The device is provided with the sample pad, so that a to-be-detected sample can be quickly sucked, instant detection of food-borne pathogen toxins is realized, the detection time is remarkably shortened, and specific antibodies on the multi-specification detection belt are utilized to play a role in high-sensitivity and specific detection of the food-borne pathogen toxins; the purpose of detecting food-borne pathogenic bacteria toxins of different types and concentration ranges is further achieved, through cooperative use of a detection line, a contrast line and a signal indication module, the effect that color changes are displayed through the signal indication module is achieved, a user can visually judge a detection result, and the detection efficiency is improved. Therefore, non-professionals can easily judge the detection result.

The present invention discloses a Cu-based + The invention relates to a method for homogeneous immunodetection of foodborne pathogens by low-field nuclear magnetic resonance mediated click chemistry gel system, which is characterized by comprising the following steps: 1) binding a foodborne pathogen aptamer to the surface of HMSMs@SAA to obtain a signal unit HMSMs@SAA&Apt; 2) coupling an alkynyl group and an azide group to PEG and PAA to obtain gel precursors Azide-PEG-Azide and PAA-Alkyne; 3) mixing a PAA-Alkyne solution, an Azide-PEG-Azide solution, an HMSMs@SAA&Apt dispersion, and a CuSO4 solution in a volume ratio of 10:10:1:1 to obtain an LF-NMR test solution, which is then mixed with a foodborne pathogen sample to determine the concentration of the foodborne pathogen in an unknown sample. The method has the advantages of high sensitivity and accuracy, strong specificity, and simple and rapid operation.

The invention is suitable for the technical field of pathogen detection, and provides a portable food-borne pathogen detection device and a detection method thereof. The device comprises an integrated magnetic frame; the chemical heating module comprises a heating cylinder, a disposable heating bag, a supporting pad and a heat conducting piece, and the disposable heating bag spontaneously releases heat through iron powder oxidation reaction; the hand-cranking energy supply module comprises a hand-cranking generator and a blue light LED lamp, and the hand-cranking generator drives the blue light LED lamp to emit light; the optical detection module comprises an optical detection piece, a transparent observation window and an optical filter, exciting light emitted by the blue light LED lamp acts on the reaction system to enable the reaction system to generate a fluorescence signal, and the fluorescence signal is observed by naked eyes after stray light is filtered out through the optical filter. Under the condition that an external power source and a cold chain are not needed, rapid on-site detection with high sensitivity and good stability is achieved, and the method can be widely applied to the scenes of food safety monitoring, public health emergency prevention and control and the like and is particularly suitable for base-level detection and on-site deployment of public health emergencies.

The invention relates to a micro-fluidic chip capable of simultaneously detecting multiple food-borne pathogenic bacteria as well as a preparation method and application of the micro-fluidic chip, in particular to detection of the food-borne pathogenic bacteria, and belongs to the technical field of analysis and detection. The device comprises a functionalized glass slide, a PDMS chip layer and a copper net, the functionalized glass slide is located at the bottommost layer, and a PDMS chip layer in plasma bonding connection is arranged on the functionalized glass slide; a plurality of through holes are formed in the PDMS chip layer, and a copper net is placed in each through hole; a plurality of round holes with the same diameter are formed in the copper net. The micro-fluidic chip is prepared through preparation of a functionalized glass slide, preparation of a PDMS chip layer, preparation of a detection unit and training of a deep learning model and is used for detecting various food-borne pathogenic bacteria at the same time. The liquid crystal biosensor provided by the invention has the advantages of simplicity, portability, rapidness, sensitivity, low cost, no mark, visualization and the like. The method has a good practical application prospect in the fields of rapid detection and the like.