70 results about "Foodborne pathogen" patented technology

The invention relates to a technique for fast detecting food-borne pathogens based on a loop-mediated isothermal amplification, LAMP technique. A primer for detection of salmonella can augment the specific base sequence of a target gene which is the invA-GenBank (accession no. DQ644633) of the salmonella, and the primer is complementary to a part of or a complementary chain of the nucleic acid sequence on the 382-580bp loci on the target gene. The invention provides a primer unit having specificity to a specific gene fragment of the salmonella, and through detecting whether or not the detecting specimen in a reagent box of the primer unit contains the specific gene fragment of the salmonella, determines whether the salmonella exists in the specimen or not.

The invention relates to a technique for fast detecting food-borne pathogens based on a loop-mediated isothermal amplification, LAMP technique. A primer for detection of shigella can augment the specific base sequence of a target gene which is the ipaH-GenBank (accession no. M32063) of the shigella, and the primer is complementary to a part of or a complementary chain of the nucleic acid sequence on the 1095-1299bp loci on the target gene. The invention provides a primer unit having specificity to a specific gene fragment of the shigella, and through detecting whether or not the detecting specimen in a reagent box of the primer unit contains the specific gene fragment of the shigella, determines whether the shigella exists in the specimen or not.

The invention relates a technique for fast detecting food-borne pathogens based on a loop-mediated isothermal amplification, LAMP technique. A primer for diction of vibrio parahemolyticus can augment the specific base sequence of a target gene which is the tih-GenBank (accession no. AY578148) of the vibrio parahemolyticus, and the primer is complementary to a part of or a complementary chain fragment of the nucleic acid sequence on the 946-1140 loci on the target gene. The invention provides a primer having specificity to a specific gene fragment of the vibrio parahemolyticus, and through detecting whether or not the detecting specimen in a reagent box of the primer unit contains the specific gene fragment of the vibrio parahemolyticus, determines whether the vibrio parahemolyticus exists in the specimen or not.

The invention relates to a method for identifying foodborne pathogen by surface enhanced Raman spectroscopy, which comprises the following steps: detecting foodborne pathogen by surface enhanced Raman spectroscopy by using gold or silver nano sol as an enhancing reagent, and carrying out cluster analysis on the detection result, thereby identifying the foodborne pathogen. The method provided by the invention has the advantages of high sensitivity, favorable selectivity, high detection speed and low cost, is easy to operate, can realize large-scale on-line detection of foodborne pathogen, and is widely applicable to the technical fields of food safety, environmental monitoring and the like.

The present invention provides a micro-gap electrode array immune sensor which is used for detecting food-borne causal agent. The sensor comprises a micro-gap array electrode, an electrode substrate which is silylanized, an enzyme deposit which has excellent electric conductivity and an enzyme linked immunoreaction sensor based on the electrical conducting test. The invention also provides a detecting method. The invention uses the micro-gap electrode array immune sensor for detecting salmonella and bacillus coli O157:H7. The method has the advantages of high sensitivity, easy operation, portability and low cost. The invention can provide an immune detecting technique which has the advantages of high speed, practicability, low cost, high sensitivity and high flux for detection and determination of the food-borne pathogenic bacteria. The transmission of food-borne pathogenic bacteria can be controlled and prevented in time and effectively.

The invention relates a technique for fast detecting food-borne pathogens based on a loop-mediated isothermal amplification, LAMP technique. A primer for diction of comma bacillus can augment the specific base sequence of a target gene which is the ompW-GenBank (accession no. AF001009) of the comma bacillus, and the primer is complementary to a part of or a complementary chain fragment of the nucleic acid sequence on the 14-220 loci on the target gene. The invention provides a primer having specificity to a specific gene fragment of the comma bacillus, and through detecting whether or not the detecting specimen in a reagent box of the primer unit contains the specific gene fragment of the comma bacillus, determines whether the comma bacillus exists in the specimen or not.

The invention relates a technique for fast detecting food-borne pathogens based on a loop-mediated isothermal amplification, LAMP technique. A primer for diction of colon bacillus O157:H7can augment the specific base sequence of a target gene which is the rfbE-GenBank (accession no. AE005429) of the colon bacillus O157:H7, and the primer is complementary to a part of or a complementary chain fragment of the nucleic acid sequence on the 7462-7471 loci on the target gene. The invention provides a primer having specificity to a specific gene fragment of the colon bacillus O157:H7, and through detecting whether or not the detecting specimen in a reagent box of the primer unit contains the specific gene fragment of the colon bacillus O157:H7, determines whether the colon bacillus O157:H7 exists in the specimen or not.

The present invention provides methods and compositions for preventing or inhibiting human food-borne pathogens in animals, and methods for increasing feed efficiency in animals by administering to the animal effective amounts of probiotic lactic acid producing bacteria. Further provided are feed compositions comprising probiotic lactic acid producing bacteria. A preferred probiotic lactic acid producing bacteria is Lactobacillus acidophilus strain ATCC accession number PTA-5249. This bacterial strain inhibits nalidixic acid-resistant Escherichia coli 0157:H7.

The invention relates a technique for fast detecting food-borne pathogens based on a loop-mediated isothermal amplification, LAMP technique. A primer for diction of staphylococcus aureus can augment the specific base sequence of a target gene which is the nuc-GenBank (accession no. V01281) of the staphylococcus aureus, and the primer is complementary to a part of or a complementary chain fragment of the nucleic acid sequence on the 548-795loci on the target gene. The invention provides a primer having specificity to a specific gene fragment of the staphylococcus aureus, and through detecting whether or not the detecting specimen in a reagent box of the primer unit contains the specific gene fragment of the staphylococcus aureus, determines whether the staphylococcus aureusexists in the specimen or not.

Provided are processes for the preparation of compositions enriched in phenolic compounds from a crude plant extract. One process includes a novel column purification step using a polymer resin that releasably adsorbs the phenolic compounds but does not retain polar non-phenolic compounds, wherein the resin comprises aromatic rings substituted with one or more electron-withdrawing groups. This invention also includes compositions enriched in phenolic compounds. This invention encompasses methods of using the phenolic-enriched compositions for treating warm-blooded animals, including humans, infected with paramyxovaridae such as respiratory syncytial virus, orthomyoxovaridae such as influenza A, B, and C, parainfluenza, Herpes viruses such as HSV-1 and HSV-2, and Flaviviruses such as West Nile Virus, and for treating inflammation such as caused by arthritis, stress and digestive disease. The compositions are also useful as meat additives to inhibit food-borne pathogens.

The invention discloses a broad-spectrum antibacterial Bacillus amyloliquefaciens strain and application thereof, and belongs to the technical field of microbes. The strain is named as Bacillus amyloliquefaciens JFL21, and is collected in the Guangdong Microbial Culture Collection Center on November 5, 2018, with the collection number of GDMCC NO: 60473. The Bacillus amyloliquefaciens JFL21 is cultured under a simple culture condition, is easy to preserve and strong in stress resistance, is easily produced industrially, and has good development and application prospects; a fermented supernatant thereof has broad-spectrum antibacterial activity for 19 common foodborne pathogens and aquatic pathogens, has extremely stable bacteriostatic performance for Listeria monocytogenes and Aeromonas hydrophila, can be further developed as a probiotic, a biological preservative or an antibacterial medicine, and is applied to food preservation or prevention of aquaculture diseases, so that use of antibiotics is reduced to a certain extent, and an application prospect is broad.

The invention belongs to the field of chemical analysis, in particular to a micro-droplet electrochemical sensor for detecting food-borne microorganisms and a preparation method thereof. Specific genefragments of the food-borne microorganisms are subjected to trace detection by a double-signal amplification system strategy to achieve signal amplification in the first step in the presence of a target gene fragment; a second DNA walker opens a third probe to form double-stranded DNA which is dissociated by a fourth probe modified with ferrocene at one end through two-strand displacement to release the DNA walker for use in the next cycle, thereby achieving signal amplification in the second step. According to the invention, the detection efficiency is improved by a double signal amplification technology, the signal stability is achieved, and the target escherichia coli O157:H7 specific gene fragment detection limit is reduced, the problems of the long period and complicated operation ofthe conventional detection of food-borne pathogens are solved; the detection accuracy is improved, and false positive detection is effectively avoided; and moreover, the electrochemical sensor has asimple production process and a wide application prospect.

A process for inhibiting foodborne pathogens and reducing foodborne disease involves contacting a living animal, a dressed carcass, or a cut of meat with an effective amount of a bacillus strain exhibiting antibacterial activity. Strains of antibacterial bacillus that are particularly effective for inhibiting Vibrio were discovered. These include Bacillus licheniformis OBT 712 and Bacillus amyloliquefaciens OBT 618.

The present invention relates to a fast analytical technique on foodborn pathogens based on loop-mediated isothermal amplification (LAMP), and provides a primer (or primers) which is (are) used for analysis on G3 of group A of rotavirus, and capable of amplifying the specific base sequence of the target gene, which is the VP7 gene (accession number of GenBank: D86272) of G3 of group A of rotavirus, and the primer(s) is (are) complemented with a partial sequence on the sites from 5095 to 5319 of the target gene, or with the complementary chain of the partial sequence. The present invention provides a group of primers or a primer with specificity on the specific gene segments of G3 of group A of rotavirus, with the utilization of the kit comprising the group of primers to analyze whether a specific gene segment of G3 of group A of rotavirus exists in the sample under analysis, thereby identifying whether G3 of group A of rotavirus exists in the sample.

The present invention relates to a primer of detecting a food-borne pathogen, and to a method of detection for food poisoning. In particular, the invention relates to a PCR primer which is specific to and is used for rapid and accurate detection of the Salmonella spp., Staphylococcus aureus, E. coli O-157, Listeria monocytogenes, and Vibrio parahemoliticus, respectively, and a detection method and a kit for the Food poisoning by using the PCR primer. Using the detection method according to the present invention, 100 to 10 CFU / ml of food borne pathogen can be detected, and rapid survey of food poisoning research can be performed within five (5).

The invention relates to a high-flux loop-mediated isothermal amplification foodborne pathogen identification apparatus and a detection method thereof. The apparatus comprises an isothermal structure (1), a reaction plate (2), a fluorescence excitation device (3), an image shooting mechanism (4) and a portable computer (5), wherein the reaction plate (2) is arranged at the top of the isothermal structure (1), the fluorescence excitation device (3) is arranged above the reaction plate (2), the image shooting mechanism (4) is arranged above the fluorescence excitation device (3), and the portable computer (5) is connected with the image shooting mechanism (4). The above cheap image acquiring device connected with the portable computer is used to shoot the fluorescence intensity in the loop-mediated isothermal nucleic acid amplification process, and analysis is carried out to detect the foodborne pathogenic bacteria. The high-flux loop-mediated isothermal amplification foodborne pathogen identification apparatus has the advantages of small size, realization of battery power supply, and completion of high-flux nucleic acid identification of a sample through field work under commercial power-free conditions. The detection method is simple to operate, and is convenient and practical.

The present invention relates to concentrating disease causing agents, such as foodborne pathogens, from complex media to expedite their detection. In particular, the present invention relates to a method to pre-concentrate pathogens rapidly, thereby enabling earlier detection times. Primarily, the present invention utilizes an approach that can concentrate the pathogens by flowing a sample through immuno-capturing tubes (“entrapment chamber” or “chamber”) during an early pre-enrichment period. Also, the invention relates to using binding materials to trap disease causing agent that is desired to be removed from the complex media such as the blood of a patient. It also related to using lights of specific wavelength to inactivate pathogens. The light is used to activate reactive oxygen species using a photo-sensitizer or directly kill the pathogen using light of wavelength between 100 nm and 450 nm.

The invention relates to an immunocapture PCR (Immuno-capturePCR, IC-PCR) detection kit for one of the four major food-borne pathogenic bacteria at the molecular level of Listeria monocytogenes (Listeriamonocytogenes, LM). For the routine detection of Listeria monocytogenes in food. The kit mainly includes: PCR tube coated with LM monoclonal antibody, PCRBuffer (10×) tube containing Mg2+, 2.5mMdNTP tube, 5UTaq DNA polymerase tube, 10pmol / μL primer 5′-CGGAGGTTCCGCAAAAGATG-3′ and 5′-CCTCCAGAGTGATCGATGTT 1 tube for each of -3', sterilized ddH2O tube, positive control tube, negative control tube, 1 tube of PBST washing buffer. The advantage of this kit is that it does not need to extract the DNA step, and the test result can be obtained by using the IC-PCR detection kit directly within 7 hours, and the target bacteria can be detected sensitively, specifically and quickly.

The present invention relates to growth inhibition of microorganisms by lactic acid bacteria. The present invention also relates to the reduction and / or treatment of food-borne pathogen infections and / or nosocomial infections. The present invention also relates to the inhibition of spoilage microorganisms in food products. The present invention further relates to the modulation of gut flora. The invention is also directed to the inhibitory potential of a whey obtained from lactic acid bacteria.

A heating assembly designed for reducing foodborne pathogens in bagged food products comprises a heat insulated treatment chamber, multiple high temperature natural gas fired furnaces, multiple insulated ductworks further comprising multiple sets of intake ducts and multiple sets of exhaust ducts, a cooling system that allows air to be controlled and supplied into the treatment chamber, multiple temperature sensor probes placed in the treatment chamber, the natural gas fired furnaces, and the bagged food products, a digital control system, and a computer program that monitors the temperature of each probe. A specially designed racking system with multiple levels allows multiple dry bags containing the food products to be stacked in a manner that rapidly and uniformly heat the food products.

The present invention relates to the technical field of food additives and particularly relates to a preservative composition and a use thereof. Every 1000 ml of solvent contains 0.005-0.1 g ethyl p-hydroxybenzoate and 0.005-0.1 g of ethylene diamine tetraacetic acid and is prepared by mixing the two materials. The preservative composition contains the ethyl p-hydroxybenzoate and the ethylene diamine tetraacetic acid at a mass ratio of 0.5-1 : 0.5-1. Compared with the prior art, the preservative composition can produce a strong inhibitory effect for the foodborne pathogen biofilm. The present invention provides a new use of the preservative composition in inhibiting the formation of foodborne pathogen biofilm. The preservative composition can be used for the preparations of drugs, food additives and feed additives inhibiting foodborne pathogen biofilm formation,, provides a new path for the control and clearance of biofilm in the food industry, and thereby provides a reliable protection for food safety.

Disclosed are novel primers for use in the molecular detection of food-threat agents and food-borne pathogens. The primers may be used in combination for the rapid, high-throughput screening PCR-based techniques to simultaneously detect multiple food safety biothreat agents. The multiplex-detection methods have improved sensitivity and specificity for the detection of multiple high-impact food-borne pathogens simultaneously. Real-time PCR assaying techniques using such primers include microarrays and multiplex single-tube arrays, the latter optionally simultaneously with TaqMan probes.

The invention relates to a kit for detecting shiga-toxigenic escherichia coli in food. The shiga-toxigenic escherichia coli is a novel high-pathogenicity foodborne pathogen carrying one or two kinds of prophage-encoded shiga toxin genes. In a detection method in the prior art, time and labor are consumed, and detection is inconvenient; the kit for specifically detecting the shiga-toxigenic escherichia coli contains an aptamer specifically combined with the shiga-toxigenic escherichia coli, wherein the aptamer is single-chain DNA. The kit has the advantages of being short in detection time and development cycle, stable in quality, easy to operate and the like and is widely applied to food and sanitary safety detection.

The present invention relates to a fast analytical technique on foodborn pathogens based on loop-mediated isothermal amplification (LAMP), and provides primers which are used for analysis on GII of noro-virus, and capable of amplifying the specific base sequence of the target gene, which encodes a capsid protein (accession number of GenBank: X86557) of GII of noro-virus, and the primer is complemented with a partial sequence on the sites from 5095 to 5319 of the target gene, or with the complementary chain of the partial sequence. The present invention provides a group of primers with specificity on the specific gene segments of GII of norovirus, with the utilization of the kit comprising the group of primers, to analyze whether a specific gene segment of GII of noro-virus exists in the sample under analysis, thereby identifying whether GII of norovirus exists in the sample.

The invention discloses an NMR rapid detection method for food-borne pathogenic bacteria based on a paramagnetic nanometer Fe-Ni-Co alloy probe, belonging to the technical field of rapid detection of food-safe pathogenic bacteria. The present invention relies on the established NMR detection method that can be used for pathogenic bacteria in food samples, utilizes the influence of the paramagnetic properties of the paramagnetic nanometer Fe-Ni-Co alloy probe on the relaxation time of the NMR attenuation signal, and detects Determine whether the target bacteria are present in the sample. The different specific correspondences are: the paramagnetic nano-Fe-Ni-Co alloy probe shows a linear relationship under certain conditions, that is, the content of the nano-Fe-Ni-Co alloy is large, and the spin-lattice relaxation time of the sample The smaller the value of spin-spin relaxation time and spin-spin relaxation time, the target bacteria can be quantitatively detected in a certain range. The method can be used for rapid detection of harmful pathogenic bacteria in food samples, and thus can be used as a rapid screening of a large number of samples to be tested.