36 results about "Foodborne pathogen" patented technology

A method for rapidly detecting and identifying food-borne pathogens by using a two-dimensional / zero-dimensional MXene / GQDs Schottky heterojunction fluorescence sensor array based on machine learning comprises the following steps: synthesizing three nitrogen-doped graphene quantum dots through a hydrothermal method, determining the optimal excitation wavelength, concentration, reaction temperature and pH value of the nitrogen-doped graphene quantum dots, and further determining the fluorescence intensity of the nitrogen-doped graphene quantum dots under the optimal condition; constructing a fluorescence sensor array, mixing the three graphene quantum dots with target heavy metal ions, incubating, then measuring fluorescence intensity to obtain fluorescence response data, and normalizing the data to obtain the fluorescence response data. And analyzing by using machine learning methods of a decision tree, a support vector machine, K nearest neighbor, linear discriminant analysis, naive Bayes and a neural network. The prepared fluorescence sensor array can rapidly identify heavy metal ions, is excellent in accuracy, sensitivity and stability, provides an economical and efficient solution for heavy metal ion detection, and has remarkable application potential in environmental monitoring.

The invention relates to the technical field of aquatic product quality safety detection, in particular to an aquatic product quality safety detection device for rapidly screening food-borne pathogenic bacteria. Comprising a workbench, a support, a suction head bin, a sample frame, a detection module, a suction head recovery box, a movement module and a pipetting module, the support is fixedly arranged at the top of the workbench, and the suction head bin, the sample frame, the detection module and the suction head recovery box are arranged between the workbench and the support and are sequentially arranged from left to right; the movement module is fixedly arranged at the inner top of the bracket, and the pipetting module is movably arranged below the movement module; an integrated, processized and automatic detection platform is constructed, the complete process from suction head installation, sample / reagent suction, liquid transfer and distribution to final suction head discarding is automatically completed, instability and fatigability of manual operation are avoided, and the overall detection efficiency and consistency are remarkably improved.

This invention discloses a strain of Salmonella choleraesuis phage SCP512 and its applications, belonging to the field of microbial technology. Salmonella choleraesuis phage SCP512 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 47021, dated February 4, 2026. It belongs to the family Myocaudae within the class Caudata. This phage SCP512 possesses multivalent lytic characteristics, excellent biological properties and environmental adaptability, and can disrupt pathogen biofilm formation. It addresses the technical problems of existing phages, such as limited lytic spectrum, insufficient coverage of common foodborne pathogens, weak biofilm removal ability, and limited application potential in practical food matrices.

This invention discloses a lactic acid bacteria bacteriocin, its preparation method, and its applications, belonging to the field of microbial technology. The invention extracts a bacteriocin from *Lactobacillus pentosus* with accession number CCTCC NO: M 2022431. This bacteriocin exhibits thermal stability, can withstand low temperatures, and maintains its activity over a wide pH range. Simultaneously, this bacteriocin can inhibit the growth and proliferation of the foodborne pathogen *Listeria monocytogenes*. Because it originates from edible Sichuan pickled vegetables, this bacteriocin is harmless to humans and can provide a new research direction for the prevention or treatment of diseases caused by foodborne pathogens, thus showing promising application prospects.

The application discloses five kinds of high-throughput detection primer groups, kits and application of LAMP method for foodborne pathogenic bacteria, and belongs to the technical field of foodborne pathogenic bacteria detection. The primer groups correspond to five kinds of bacteria, including Salmonella, Vibrio parahaemolyticus, Listeria monocytogenes, Escherichia coli O157:H7 and Staphylococcus aureus, and the nucleotide sequences of the primers are SEQ ID No. 1-30. The complete process detection can be completed at 62 DEG C constant temperature for 45 minutes, the result does not need complex instruments, the positive gray and the negative purple are judged by naked eyes, and the operation is simple. The detection sensitivity reaches 1-75 copies / reaction, the artificially contaminated aquatic product sample does not need to be subjected to bacterial enrichment, is subjected to simple lysis and enrichment, has strong specificity and no cross reaction. The anti-interference ability is outstanding, the complex matrix of aquatic products is adapted, the detection coincidence rate is high, the cost is low, only a conventional constant temperature device is needed, and the application is suitable for multiple scenes, such as detection institutions, food enterprises and on-site law enforcement on-site detection of primary food, agriculture, disease control, customs and the like, and provides an efficient and economical technical means for aquatic product safety control.

This invention discloses a quadruple Taqman qPCR reagent for detecting foodborne pathogens in aquatic products. The foodborne pathogens are Vibrio parahaemolyticus, Vibrio vulnificus, Shigella, and Staphylococcus aureus. The reagent includes specific primers and Taqman probes corresponding to the four foodborne pathogens. This invention provides a quadruple Taqman qPCR reagent with high efficiency, accuracy, sensitivity, and speed, offering an accurate, rapid, efficient, and economical method for detecting the above four foodborne pathogens in the quality and safety testing of aquatic products and their processed products. This is of great significance for ensuring the safety of aquatic products for consumption in my country.

The application relates to the technical field of detection, in particular to a general foodborne pathogenic bacteria detection method suitable for a long-distance sailing environment. UiO-66 is used to enrich methylene blue, and is combined with target bacteria aptamer to obtain UiO-66 / MB / Aptamer which simultaneously serves as a signal amplification and recognition probe. A membrane separation method is used to filter out the probes not combined with the bacteria. The electrochemical signal of methylene blue is detected by using a differential pulse voltammetry method, a corresponding linear relationship is constructed, and the concentration of the bacteria is quantitatively detected. The application not only guarantees the sensitivity, detection time, portability and other characteristics of the detection of foodborne pathogenic bacteria, but also provides a general detection method for detecting various target bacteria. Through the reproducibility and stability test under the high-temperature, high-humidity and high-salt environment, it is proved that the application can realize the rapid, sensitive and stable detection of foodborne pathogenic bacteria in a complex long-distance sailing environment.

This invention relates to an aptamer of *Cronobacter sakazakii* and a method for preparing a fluorescence-quenched biosensor thereof, belonging to the field of biosensor technology. The DNA sequence of the *Cronobacter sakazakii* aptamer is shown in SEQ ID No:1. Experiments show that the cross-reactivity of this aptamer with common foodborne pathogens such as *Staphylococcus aureus* and *Listeria monocytogenes* is less than 25%. The construction process of the fluorescence-quenched biosensor based on the *Cronobacter sakazakii* aptamer includes the mixing of activating agents, the preparation of fluorescent probes and quenching probes, and the mixing of fluorescent probes and quenching probes in a volume ratio to obtain a sensitive and specific fluorescence-quenched biosensor. The fluorescence-quenched biosensor of this invention exhibits high linearity and low detection limits; simultaneously, the screening method is simple and convenient, requiring only the use of an existing Autodoc server for rapid screening.

An example system includes a light intensity measuring apparatus couplable to a food processing apparatus and a computing system. The light intensity measuring apparatus includes a chamber configured to receive a water sample from the food processing apparatus, a light source, a detector configured to detect light that has passed through the water sample and measure multiple times intensities of wavelengths of the light to obtain multiple sets of measured intensities of wavelengths, and a communication module configured to provide the multiple sets of measured intensities of wavelengths. The computing system may receive the multiple sets of measured intensities, process the multiple sets to obtain a set of values, apply a first set of decision trees to the set of values to obtain a first result indicating a positive or negative foodborne pathogen detection, generate a notification indicating either the positive of negative detection, and provide the notification.

This invention relates to a composite material and its preparation method for adsorbing and catalytically degrading foodborne pathogens, belonging to the field of composite material technology. The composite material for adsorbing and catalytically degrading foodborne pathogens comprises a porous nanocarrier, a photocatalytically active component loaded on the porous nanocarrier, and nano-silver modified on the surface of the photocatalytically active component; wherein the porous nanocarrier is graphene oxide; the photocatalytically active component is titanium dioxide and zinc oxide in a mass ratio of 2:1 to 1:2; and the mass ratio of the photocatalytically active component to the porous nanocarrier is 1:5 to 1:1. The composite material of this invention possesses both high-efficiency, broad-spectrum antibacterial properties and long-term stability, and can be widely used in the food antibacterial industry.

This invention relates to the field of food packaging materials technology, and provides a silver-bipyridine column[5]arene coordination polymer / chitosan composite film, its preparation method, and its application. The silver-bipyridine column[5]arene coordination polymer / chitosan composite film of this invention achieves long-term inhibition of common foodborne pathogens through a dual mechanism of direct antibacterial action by silver ions and peroxidase-like catalysis enhanced by column[5]arene, combined with a stable coordination polymer network constructed from bipyridine bifunctionalized column[5]arene. It can block harmful ultraviolet rays to prevent food photo-oxidation and maintain high light transmittance for easy observation. Relying on its long-term antibacterial effect and ultraviolet blocking function, it can extend the shelf life of food, maintain its freshness and nutritional value. Using biodegradable chitosan as a matrix, it meets the development requirements of green packaging.

This invention belongs to the field of food packaging technology, specifically relating to a biomimetic breathable preservation film, its preparation method, and its application. The invention uses polylactic acid as a base material, adding two inorganic modified compounds: amino acid-functionalized cobalt-aluminum-gallium ternary layered bimetallic hydroxide nanosheets and an iron-copper bimetallic organic framework material loaded with silver nanoparticles. Plasticizers and surfactants are also added, and the film is prepared through solution blending, low-temperature ultrasonic dispersion, and casting. This preservation film possesses biomimetic breathable functionality, with oxygen and carbon dioxide permeability within the suitable range for modified atmosphere preservation of fruits and vegetables. The carbon dioxide to oxygen permeability ratio is also reasonable, exhibiting efficient ethylene removal capabilities and broad-spectrum antibacterial activity against common foodborne pathogens. Using this preservation film for fruit and vegetable packaging can significantly extend the shelf life of fresh agricultural products such as grapes and blueberries. The preparation process of this invention is simple and has broad application prospects in the field of active food packaging.

This invention provides an antimicrobial peptide that inhibits foodborne pathogens, its acquisition method, and its applications, relating to the fields of bioactive peptides and biomedical engineering technology. The amino acid sequence of the antimicrobial peptide is: Gly-Leu-Thr-Leu-Lys-His-Leu-Lys-Lys-Leu-Ile-Phe. This antimicrobial peptide can effectively inhibit the growth of foodborne pathogens such as Staphylococcus aureus, Salmonella, Escherichia coli, Pseudomonas aeruginosa, and Listeria monocytogenes. This invention overcomes the shortcomings of existing technologies by using a screening method combining gastrointestinal digestive enzyme-peptidomics technology and experiments to discover and identify antimicrobial peptides with antimicrobial activity, providing new natural antimicrobial agents for the food, pharmaceutical, and other related fields.

This invention discloses a laser-irradiated *Lactobacillus amylovorus* and its application in controlling foodborne pathogens, belonging to the field of microbial technology. The *Lactobacillus amylovorus* LY2332 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37009. This invention induces metabolic remodeling in *Lactobacillus amylovorus* through laser irradiation, effectively enhancing its environmental adaptability and the antibacterial activity of its cell-free supernatant, and demonstrating good application results in the preservation of chilled meat. This invention not only clarifies that laser irradiation can enhance the antibacterial activity of cell-free supernatant of lactic acid bacteria, but also provides a new strategy for developing natural food control agents and lays a theoretical foundation for the industrial application of lactic acid bacteria in food preservation.

This invention relates to the field of gene detection, specifically to a single-tube multiplex detection sequence combination, kit, detection method, and application for foodborne pathogens based on asymmetric ratios and melting curves. This invention provides a sequence combination for detecting foodborne pathogens, comprising a forward primer, a reverse primer, a specific probe, and a reverse partially complementary melting curve probe. This invention also provides a kit for detecting foodborne pathogens, which, through adjustment of the asymmetric ratios, solves the problems of systemic competitive inhibition and cross-matching in complex genetic backgrounds, constructing a single-tube detection method for five types of diarrheal Escherichia coli, Salmonella, Listeria monocytogenes, and Staphylococcus aureus. This method does not require additional complex hardware channels or expensive detection equipment, and has the advantages of high throughput, strong specificity, and low instrument cost, making it suitable for application and promotion in low-resource grassroots institutions, customs, and front-line food safety supervision.

The invention is suitable for the technical field of pathogen detection, and provides a portable food-borne pathogen detection device and a detection method thereof. The device comprises an integrated magnetic frame; the chemical heating module comprises a heating cylinder, a disposable heating bag, a supporting pad and a heat conducting piece, and the disposable heating bag spontaneously releases heat through iron powder oxidation reaction; the hand-cranking energy supply module comprises a hand-cranking generator and a blue light LED lamp, and the hand-cranking generator drives the blue light LED lamp to emit light; the optical detection module comprises an optical detection piece, a transparent observation window and an optical filter, exciting light emitted by the blue light LED lamp acts on the reaction system to enable the reaction system to generate a fluorescence signal, and the fluorescence signal is observed by naked eyes after stray light is filtered out through the optical filter. Under the condition that an external power source and a cold chain are not needed, rapid on-site detection with high sensitivity and good stability is achieved, and the method can be widely applied to the scenes of food safety monitoring, public health emergency prevention and control and the like and is particularly suitable for base-level detection and on-site deployment of public health emergencies.

The invention relates to a micro-fluidic chip capable of simultaneously detecting multiple food-borne pathogenic bacteria as well as a preparation method and application of the micro-fluidic chip, in particular to detection of the food-borne pathogenic bacteria, and belongs to the technical field of analysis and detection. The device comprises a functionalized glass slide, a PDMS chip layer and a copper net, the functionalized glass slide is located at the bottommost layer, and a PDMS chip layer in plasma bonding connection is arranged on the functionalized glass slide; a plurality of through holes are formed in the PDMS chip layer, and a copper net is placed in each through hole; a plurality of round holes with the same diameter are formed in the copper net. The micro-fluidic chip is prepared through preparation of a functionalized glass slide, preparation of a PDMS chip layer, preparation of a detection unit and training of a deep learning model and is used for detecting various food-borne pathogenic bacteria at the same time. The liquid crystal biosensor provided by the invention has the advantages of simplicity, portability, rapidness, sensitivity, low cost, no mark, visualization and the like. The method has a good practical application prospect in the fields of rapid detection and the like.

This invention provides a strain of Bacillus mojavensis, belonging to the field of microbial technology. The Bacillus mojavensis VT275 of this invention, with accession number CGMCC No. 29974, can effectively inhibit common skin pathogens and foodborne pathogens, such as Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Staphylococcus epidermidis, Listeria monocytogenes, Shigella, and Enterococcus faecalis. The Bacillus mojavensis VT275 of this invention can be used to prevent or treat skin diseases such as atopic dermatitis or to control foodborne infections, and has good application prospects in the fields of pharmaceuticals, cosmetics, edible microorganisms, and feed microorganisms.

This utility model relates to the technical field of rapid detection devices for foodborne pathogens, and discloses a portable rapid detection device for foodborne pathogens, including a storage box and a detector. The storage box has a rotatable lid on top, and a placement slot is provided inside the storage box. Both ends of the placement slot have fixing slots, and the detector is placed inside the placement slot. Pull rods are fixedly installed inside each of the two fixing slots, and support plates are fixedly installed at the bottom ends of the two pull rods. In this utility model, by designing a liftable support plate at the bottom of the detector, and placing the detector on top of the support plate, the detector can be removed from the storage box by pulling the fixing frame upwards to compress the spring. Then, by releasing the fixing frame, the support plate is reset by the rebound force of the springs at the outer ends of the two pull rods. This structural design allows for faster removal of the detector, saving working time and improving the efficiency of the entire detection process.

The application discloses a colorimetric detection method for foodborne pathogenic bacteria based on Fe3O4@MIL-100(Fe) magnetic separation and nanocatalysis, which mainly comprises the following steps: preparing Fe3O4@MIL-100(Fe) material through a solvothermal method and an in-situ growth method, and modifying the material with an aptamer to obtain Fe3O4@MIL-100(Fe)@aptamer, so as to realize the capture and magnetic separation of foodborne pathogenic bacteria. The nanocatalytic activity of the Fe3O4@MIL-100(Fe)@aptamer is utilized, TMB is used as a substrate to catalyze a product, and foodborne pathogenic bacteria can be rapidly detected. The method disclosed by the application has the advantages of high detection sensitivity, strong specificity, short time consumption and the like, and is suitable for rapid detection of foodborne pathogenic bacteria in food samples.

Vibrio parahaemolyticus serotype O10 is an important foodborne pathogen with a very high isolation rate in my country in recent years. This invention provides a specific oligonucleotide sequence for detecting Vibrio parahaemolyticus serotype O10 and its application. The oligonucleotides are DNA fragments selected from the IS1 and orf9 genes of the O antigen gene cluster of Vibrio parahaemolyticus serotype O10. This invention also establishes a PCR detection method for Vibrio parahaemolyticus serotype O10 using the above-mentioned oligonucleotides, establishing for the first time a technique for detecting this bacterium using molecular biology methods. This is of great significance for the identification and epidemiological monitoring of this important foodborne pathogen.

This invention discloses the application of UV-regulated Fe / Lyz@CDs nanozymes in fruit preservation. Using lysozyme and diethylenetriaminepentaacetic acid as precursors, Fe-doped lysozyme-based carbon dot (Fe / Lyz@CDs) nanozymes were prepared via a simple hydrothermal method. The Fe / Lyz@CDs nanozymes exhibit UV-responsive peroxidase (POD) and superoxide dismutase (SOD)-like catalytic properties. Fe / Lyz@CDs catalyze the generation of reactive oxygen species (ROS), which are beneficial for *Escherichia coli* (E. coli). E.coli Staphylococcus aureus ( S.aureus Fe / Lyz@CDs exhibits significant bactericidal effects against four typical foodborne pathogens: multidrug-resistant Escherichia coli (AREC) and methicillin-resistant Staphylococcus aureus (MRSA). Fe / Lyz@CDs is dispersed in a polyvinyl alcohol (PVA) matrix to prepare Fe / Lyz@CDs-PVA film. Using this film for mango preservation can maintain the quality of mangoes and extend their storage time.

This invention discloses an antimicrobial peptide, sesquimycin, and its applications. Specifically, it involves eight sesquimycin-like polypeptide compounds. Compounds 1-8 exhibit varying degrees of antimicrobial activity, with compound 2 possessing broad-spectrum antimicrobial activity. Its minimum inhibitory concentration (MIC) against *Staphylococcus aureus* ATCC 6538 and *Bacillus cereus* CMCC(B)63301 reaches 20 μg / mL, and its MIC against *Enterococcus faecalis* 160119481, *Salmonella typhi* CMCC(B)50071, and *Salmonella typhimurium* CMCC(B)50115 is 40 μg / mL. Given that *Staphylococcus*, *Bacillus*, *Enterococcus*, and *Salmonella* are common foodborne pathogens posing a serious threat to food safety, compounds 1-8 can be used to prepare novel antimicrobial peptides for application in the field of food safety preservation.

The invention discloses a method for detecting food-borne pathogenic bacteria based on Argonaute protein in combination with loop-mediated isothermal amplification and electrochemical biosensing, and belongs to the technical field of microbiological detection and biosensing. The integrated detection method based on Argonaute protein, loop-mediated isothermal amplification and electrochemical biosensing, which is high in sensitivity, strong in specificity, simple and convenient to operate and suitable for on-site rapid detection, is provided aiming at the problems that an existing food-borne pathogenic bacterium detection method is insufficient in sensitivity, depends on complex instruments, is difficult to apply on site and the like. Target nucleic acid is subjected to exponential amplification through LAMP (loop-mediated isothermal amplification), then Argonaute-mediated two-stage specific recognition and signal transduction are performed, and finally reading is performed through a high-sensitivity electrochemical platform, so that the detection limit of salmonella typhimurium as high as 1 CFU / mL is realized, and the detection range can cover 100-108 CFU / mL.

This invention relates to the field of biomedicine, specifically to an antibody for detecting Escherichia coli O45 and its application method. The antibody is a monoclonal antibody or its antigen-binding fragment, comprising the heavy chain CDR sequence shown in SEQ ID NO:1-3 and the light chain CDR sequence shown in SEQ ID NO:4-6. This invention also provides the nucleic acid molecule encoding the antibody, the expression vector, the host cell, and the preparation method thereof. Specificity identification results show that the antibody specifically binds to Escherichia coli O45 and shows no cross-reactivity with other Escherichia coli serotypes such as O157, O26, O103, O111, O121, and O145, as well as closely related serotypes of O55, and also shows no cross-reactivity with common foodborne pathogens. The indirect ELISA detection method based on this antibody has a detection limit of 1×10⁻⁶ for Escherichia coli O45. 2 The detection rate is CFU / mL, and the entire testing process can be completed within 24 hours. The monoclonal antibody provided by this invention can be used to prepare detection kits, test strips, and other products, and is suitable for rapid screening of O45 Escherichia coli in imported and exported food.

This invention discloses a strain of *Pediococcus pentosaceus* and its applications, belonging to the field of microbial technology. The strain is *Pediococcus pentosaceus* (…). Pediococcus acidilactici L27, with accession number CCTCC NO: M 20252576, has the following outstanding characteristics: (1) strong cholesterol-lowering ability in vitro; (2) excellent tolerance to the gastrointestinal environment, maintaining a high survival rate in simulated gastric juice and bile salt-containing environments; (3) able to produce broad-spectrum antibacterial substances mainly composed of organic acids, which can inhibit a variety of foodborne pathogens and have stable antibacterial properties; (4) possessing hydrophobic cell surface and co-aggregation ability with pathogens, showing potential for intestinal colonization competition; and (5) having no hemolytic activity and good safety. The L27 strain of this invention can be used to prepare drugs, fermented dairy products, probiotic preparations or feed additives with cholesterol-lowering and / or intestinal flora regulation functions, providing a core strain resource for the development of multifunctional, efficient and stable new probiotic products.

This invention proposes a droplet digital PCR method for simultaneously detecting the quantity of four foodborne pathogens. The steps include: 1. Preparing the DNA sample to be tested; 2. Preparing primers and probes for the four bacteria to be tested; the four bacteria to be tested are Staphylococcus aureus, Listeria monocytogenes, Salmonella typhi, and Bacillus cereus; 3. Using a ddPCR amplification reaction system, which includes the primers and probes for the four bacteria to be tested in step 2, amplifying the DNA sample to be tested in step 1, detecting the amplification products, and obtaining the quantitative results of the four bacteria. This invention can simultaneously detect four pathogenic microorganisms: Staphylococcus aureus, Listeria monocytogenes, Salmonella typhi, and Bacillus cereus. It establishes a precise, rapid, and absolute quantitative detection system for the simultaneous detection of these four pathogens using multiplex ddPCR technology, exhibiting high specificity, high precision, and strong repeatability.

The invention provides a bionic colorimetric sensing platform for indicating food freshness. The bionic colorimetric sensing platform comprises a substrate and N preset gel columns arranged on the substrate, each gel column is divided into an adsorption layer and a sensing layer; the adsorption layer is used for adsorbing a target detection object so as to realize enrichment and diffusion of the target detection object; one surface of the sensing layer is directly combined with the substrate, and the other surface is combined with one surface, close to the substrate, of the adsorption layer; the sensing layer is used for receiving the target detection object diffused by the adsorption layer, the sensing layer is loaded with a dyeing substance, the dyeing substance and the target detection object are subjected to colorimetric reaction, and the existence and content of the target detection object are reflected through the optical signal change of the dyeing substance. According to the application, high-sensitivity detection of characteristic decay gas and food-borne pathogens is realized, the early decay of food can be effectively evaluated, and the application is simple and convenient.

The invention relates to the technical field of detection, in particular to a portable food-borne pathogenic bacterium detection platform and a detection method. The invention provides a portable food-borne pathogen detection platform. The portable food-borne pathogen detection platform comprises the following modules: a power supply module, a heating module, a centrifugal module and a 3D printing shell, the power supply module comprises an electromagnetic valve and an air switch; the heating module comprises a heating table, a microchip, an LED display screen, a temperature sensor, a setting button and a heater; and the centrifugal module comprises a rotary sample holder and a battery box. The portable food-borne pathogen detection platform disclosed by the invention can realize on-site rapid detection of staphylococcus aureus of which the LOD is 10 CFU / mL within 1 hour. And a basis is provided for rapid detection of staphylococcus aureus.

The present application belongs to the technical field of gene detection, and aims at the fact that most of the existing methods focus on the specific report and multiplex detection capacity of pAgos, but often ignore the key problem of amplicon contamination. The present application provides a nucleic acid detection composition, kit and method for foodborne pathogens based on LAMP-PfAgo, which utilizes primers to perform LAMP amplification on foodborne pathogens, and after the reaction is completed, a probe and PfAgo are added, the PfAgo performs the first cutting on the LAMP amplification product to obtain a cutting product, and then the PfAgo performs the second cutting on the probe under the action of the cutting product to produce specific fluorescence. The primer probe combination of the present application can efficiently, specifically and sensitively detect multiple foodborne pathogens at the same time, and can effectively eliminate the problem of LAMP amplicon contamination.