70 results about "Foodborne pathogen" patented technology

The invention relates a technique for fast detecting food-borne pathogens based on a loop-mediated isothermal amplification, LAMP technique. A primer for diction of vibrio parahemolyticus can augment the specific base sequence of a target gene which is the tih-GenBank (accession no. AY578148) of the vibrio parahemolyticus, and the primer is complementary to a part of or a complementary chain fragment of the nucleic acid sequence on the 946-1140 loci on the target gene. The invention provides a primer having specificity to a specific gene fragment of the vibrio parahemolyticus, and through detecting whether or not the detecting specimen in a reagent box of the primer unit contains the specific gene fragment of the vibrio parahemolyticus, determines whether the vibrio parahemolyticus exists in the specimen or not.

Devices and methods for the detection of analytes are disclosed. Devices and methods for detecting food-borne pathogens are disclosed.

The invention relates to a method for identifying foodborne pathogen by surface enhanced Raman spectroscopy, which comprises the following steps: detecting foodborne pathogen by surface enhanced Raman spectroscopy by using gold or silver nano sol as an enhancing reagent, and carrying out cluster analysis on the detection result, thereby identifying the foodborne pathogen. The method provided by the invention has the advantages of high sensitivity, favorable selectivity, high detection speed and low cost, is easy to operate, can realize large-scale on-line detection of foodborne pathogen, and is widely applicable to the technical fields of food safety, environmental monitoring and the like.

The present invention provides a micro-gap electrode array immune sensor which is used for detecting food-borne causal agent. The sensor comprises a micro-gap array electrode, an electrode substrate which is silylanized, an enzyme deposit which has excellent electric conductivity and an enzyme linked immunoreaction sensor based on the electrical conducting test. The invention also provides a detecting method. The invention uses the micro-gap electrode array immune sensor for detecting salmonella and bacillus coli O157:H7. The method has the advantages of high sensitivity, easy operation, portability and low cost. The invention can provide an immune detecting technique which has the advantages of high speed, practicability, low cost, high sensitivity and high flux for detection and determination of the food-borne pathogenic bacteria. The transmission of food-borne pathogenic bacteria can be controlled and prevented in time and effectively.

The invention relates a technique for fast detecting food-borne pathogens based on a loop-mediated isothermal amplification, LAMP technique. A primer for diction of comma bacillus can augment the specific base sequence of a target gene which is the ompW-GenBank (accession no. AF001009) of the comma bacillus, and the primer is complementary to a part of or a complementary chain fragment of the nucleic acid sequence on the 14-220 loci on the target gene. The invention provides a primer having specificity to a specific gene fragment of the comma bacillus, and through detecting whether or not the detecting specimen in a reagent box of the primer unit contains the specific gene fragment of the comma bacillus, determines whether the comma bacillus exists in the specimen or not.

The invention relates a technique for fast detecting food-borne pathogens based on a loop-mediated isothermal amplification, LAMP technique. A primer for diction of colon bacillus O157:H7can augment the specific base sequence of a target gene which is the rfbE-GenBank (accession no. AE005429) of the colon bacillus O157:H7, and the primer is complementary to a part of or a complementary chain fragment of the nucleic acid sequence on the 7462-7471 loci on the target gene. The invention provides a primer having specificity to a specific gene fragment of the colon bacillus O157:H7, and through detecting whether or not the detecting specimen in a reagent box of the primer unit contains the specific gene fragment of the colon bacillus O157:H7, determines whether the colon bacillus O157:H7 exists in the specimen or not.

The present invention provides methods and compositions for preventing or inhibiting human food-borne pathogens in animals, and methods for increasing feed efficiency in animals by administering to the animal effective amounts of probiotic lactic acid producing bacteria. Further provided are feed compositions comprising probiotic lactic acid producing bacteria. A preferred probiotic lactic acid producing bacteria is Lactobacillus acidophilus strain ATCC accession number PTA-5249. This bacterial strain inhibits nalidixic acid-resistant Escherichia coli 0157:H7.

The invention discloses a broad-spectrum antibacterial Bacillus amyloliquefaciens strain and application thereof, and belongs to the technical field of microbes. The strain is named as Bacillus amyloliquefaciens JFL21, and is collected in the Guangdong Microbial Culture Collection Center on November 5, 2018, with the collection number of GDMCC NO: 60473. The Bacillus amyloliquefaciens JFL21 is cultured under a simple culture condition, is easy to preserve and strong in stress resistance, is easily produced industrially, and has good development and application prospects; a fermented supernatant thereof has broad-spectrum antibacterial activity for 19 common foodborne pathogens and aquatic pathogens, has extremely stable bacteriostatic performance for Listeria monocytogenes and Aeromonas hydrophila, can be further developed as a probiotic, a biological preservative or an antibacterial medicine, and is applied to food preservation or prevention of aquaculture diseases, so that use of antibiotics is reduced to a certain extent, and an application prospect is broad.

The invention belongs to the field of chemical analysis, in particular to a micro-droplet electrochemical sensor for detecting food-borne microorganisms and a preparation method thereof. Specific genefragments of the food-borne microorganisms are subjected to trace detection by a double-signal amplification system strategy to achieve signal amplification in the first step in the presence of a target gene fragment; a second DNA walker opens a third probe to form double-stranded DNA which is dissociated by a fourth probe modified with ferrocene at one end through two-strand displacement to release the DNA walker for use in the next cycle, thereby achieving signal amplification in the second step. According to the invention, the detection efficiency is improved by a double signal amplification technology, the signal stability is achieved, and the target escherichia coli O157:H7 specific gene fragment detection limit is reduced, the problems of the long period and complicated operation ofthe conventional detection of food-borne pathogens are solved; the detection accuracy is improved, and false positive detection is effectively avoided; and moreover, the electrochemical sensor has asimple production process and a wide application prospect.

The present invention relates to a fast analytical technique on foodborn pathogens based on loop-mediated isothermal amplification (LAMP), and provides a primer (or primers) which is (are) used for analysis on G3 of group A of rotavirus, and capable of amplifying the specific base sequence of the target gene, which is the VP7 gene (accession number of GenBank: D86272) of G3 of group A of rotavirus, and the primer(s) is (are) complemented with a partial sequence on the sites from 5095 to 5319 of the target gene, or with the complementary chain of the partial sequence. The present invention provides a group of primers or a primer with specificity on the specific gene segments of G3 of group A of rotavirus, with the utilization of the kit comprising the group of primers to analyze whether a specific gene segment of G3 of group A of rotavirus exists in the sample under analysis, thereby identifying whether G3 of group A of rotavirus exists in the sample.

Devices and methods for the detection of antigens are disclosed. Devices and methods for detecting food-borne pathogens are disclosed.

The present invention relates to a primer of detecting a food-borne pathogen, and to a method of detection for food poisoning. In particular, the invention relates to a PCR primer which is specific to and is used for rapid and accurate detection of the Salmonella spp., Staphylococcus aureus, E. coli O-157, Listeria monocytogenes, and Vibrio parahemoliticus, respectively, and a detection method and a kit for the Food poisoning by using the PCR primer. Using the detection method according to the present invention, 100 to 10 CFU/ml of food borne pathogen can be detected, and rapid survey of food poisoning research can be performed within five (5).

The invention relates to a high-flux loop-mediated isothermal amplification foodborne pathogen identification apparatus and a detection method thereof. The apparatus comprises an isothermal structure (1), a reaction plate (2), a fluorescence excitation device (3), an image shooting mechanism (4) and a portable computer (5), wherein the reaction plate (2) is arranged at the top of the isothermal structure (1), the fluorescence excitation device (3) is arranged above the reaction plate (2), the image shooting mechanism (4) is arranged above the fluorescence excitation device (3), and the portable computer (5) is connected with the image shooting mechanism (4). The above cheap image acquiring device connected with the portable computer is used to shoot the fluorescence intensity in the loop-mediated isothermal nucleic acid amplification process, and analysis is carried out to detect the foodborne pathogenic bacteria. The high-flux loop-mediated isothermal amplification foodborne pathogen identification apparatus has the advantages of small size, realization of battery power supply, and completion of high-flux nucleic acid identification of a sample through field work under commercial power-free conditions. The detection method is simple to operate, and is convenient and practical.

The present invention relates to concentrating disease causing agents, such as foodborne pathogens, from complex media to expedite their detection. In particular, the present invention relates to a method to pre-concentrate pathogens rapidly, thereby enabling earlier detection times. Primarily, the present invention utilizes an approach that can concentrate the pathogens by flowing a sample through immuno-capturing tubes (“entrapment chamber” or “chamber”) during an early pre-enrichment period. Also, the invention relates to using binding materials to trap disease causing agent that is desired to be removed from the complex media such as the blood of a patient. It also related to using lights of specific wavelength to inactivate pathogens. The light is used to activate reactive oxygen species using a photo-sensitizer or directly kill the pathogen using light of wavelength between 100 nm and 450 nm.

The invention relates to an immunocapture PCR (Immuno-capturePCR, IC-PCR) detection kit for one of the four major food-borne pathogenic bacteria at the molecular level of Listeria monocytogenes (Listeriamonocytogenes, LM). For the routine detection of Listeria monocytogenes in food. The kit mainly includes: PCR tube coated with LM monoclonal antibody, PCRBuffer (10×) tube containing Mg2+, 2.5mMdNTP tube, 5UTaq DNA polymerase tube, 10pmol/μL primer 5′-CGGAGGTTCCGCAAAAGATG-3′ and 5′-CCTCCAGAGTGATCGATGTT 1 tube for each of -3', sterilized ddH2O tube, positive control tube, negative control tube, 1 tube of PBST washing buffer. The advantage of this kit is that it does not need to extract the DNA step, and the test result can be obtained by using the IC-PCR detection kit directly within 7 hours, and the target bacteria can be detected sensitively, specifically and quickly.

A heating assembly designed for reducing foodborne pathogens in bagged food products comprises a heat insulated treatment chamber, multiple high temperature natural gas fired furnaces, multiple insulated ductworks further comprising multiple sets of intake ducts and multiple sets of exhaust ducts, a cooling system that allows air to be controlled and supplied into the treatment chamber, multiple temperature sensor probes placed in the treatment chamber, the natural gas fired furnaces, and the bagged food products, a digital control system, and a computer program that monitors the temperature of each probe. A specially designed racking system with multiple levels allows multiple dry bags containing the food products to be stacked in a manner that rapidly and uniformly heat the food products.

The invention relates to a kit for detecting shiga-toxigenic escherichia coli in food. The shiga-toxigenic escherichia coli is a novel high-pathogenicity foodborne pathogen carrying one or two kinds of prophage-encoded shiga toxin genes. In a detection method in the prior art, time and labor are consumed, and detection is inconvenient; the kit for specifically detecting the shiga-toxigenic escherichia coli contains an aptamer specifically combined with the shiga-toxigenic escherichia coli, wherein the aptamer is single-chain DNA. The kit has the advantages of being short in detection time and development cycle, stable in quality, easy to operate and the like and is widely applied to food and sanitary safety detection.