A rapid NMR method for the detection of foodborne pathogens based on a paramagnetic nanofe-ni-co alloy probe

A technology of food-borne pathogenic bacteria, fe-ni-co, used in the direction of measuring devices, analysis using nuclear magnetic resonance, instruments, etc.

Active Publication Date: 2016-04-13
苏州辅测技术服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no literature reporting this method at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0020] Examine food samples to determine whether they contain the harmful pathogen Listeria monocytogenes.

[0021] 1. Preparation of nano-Fe-Ni-Co alloy immunoprobes: buy nano-Fe-Ni-Co alloy materials from the market, such as Beijing Deke Daojin Technology Co., Ltd. or Shanghai Chaowei Nano Materials Co., Ltd., 20nm, ratio 1: 1:1, each with a purity of 99.5%.

[0022] Nano-Fe-Ni-Co alloy coated with silicon dioxide: take 47.5g of sodium silicate, dissolve it in a beaker with deionized water, and adjust the pH value to 12-13 with hydrochloric acid. Take 5.0g of nano-Fe-Ni-Co alloy and add it into the beaker, and stir mechanically (with a glass rod) for 5min. The mixture was sonicated for 30 min and stirred in due course. Raise the temperature to 85°C, add hydrochloric acid dropwise to adjust the pH value to 6-7, and form a precipitate. While magnetically separating, wash the precipitate with deionized water for 3-4 times. Then, the precipitate was dispersed in 250 mL of me...

example 2

[0029] Determination of food samples for the presence of harmful pathogenic bacteria Escherichia coli O157:H7.

[0030] 1. Preparation of nano-Fe-Ni-Co alloy immunoprobe: buy nano-Fe-Ni-Co alloy materials from the market, such as Beijing Deke Daojin Technology Co., Ltd. or Shanghai Chaowei Nanomaterials Co., Ltd., 20nm, ratio 1:1: 1. The respective purity is 99.5%.

[0031] Nano-Fe-Ni-Co alloy coated with silicon dioxide: take 47.5g of sodium silicate, dissolve it in a beaker with deionized water, and adjust the pH value to 12-13 with hydrochloric acid. Take 5.0g of nano-Fe-Ni-Co alloy and add it into the beaker, and stir mechanically (with a glass rod) for 5min. The mixture was sonicated for 30 min and stirred in due course. Raise the temperature to 85°C, add hydrochloric acid dropwise to adjust the pH value to 6-7, and form a precipitate. While magnetically separating, wash the precipitate with deionized water for 3-4 times. Then, the precipitate was dispersed in 250 mL...

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Abstract

The invention discloses an NMR rapid detection method for food-borne pathogenic bacteria based on a paramagnetic nanometer Fe-Ni-Co alloy probe, belonging to the technical field of rapid detection of food-safe pathogenic bacteria. The present invention relies on the established NMR detection method that can be used for pathogenic bacteria in food samples, utilizes the influence of the paramagnetic properties of the paramagnetic nanometer Fe-Ni-Co alloy probe on the relaxation time of the NMR attenuation signal, and detects Determine whether the target bacteria are present in the sample. The different specific correspondences are: the paramagnetic nano-Fe-Ni-Co alloy probe shows a linear relationship under certain conditions, that is, the content of the nano-Fe-Ni-Co alloy is large, and the spin-lattice relaxation time of the sample The smaller the value of spin-spin relaxation time and spin-spin relaxation time, the target bacteria can be quantitatively detected in a certain range. The method can be used for rapid detection of harmful pathogenic bacteria in food samples, and thus can be used as a rapid screening of a large number of samples to be tested.

Description

technical field [0001] The invention relates to a rapid detection method for pathogenic bacteria, in particular to an NMR rapid detection method for food-borne pathogenic bacteria based on nanometer Fe-Ni-Co alloy probes. Background technique [0002] Basic principle: Monoclonal antibodies or antigen molecules are combined with enzyme molecules through covalent bonds. This combination will not change the immunological characteristics and biological activities of monoclonal antibodies, antigens and enzymes. Specific monoclonal antibodies will only interact with specificity. antigen binding. Fe-Ni-Co alloy materials are ferromagnetic, and paramagnetic properties will appear when the particle diameter is small enough to a certain extent. That is, there is no magnetism when there is no external magnetic field, but it shows certain magnetism when there is an external magnetic field, which can be used for magnetic separation. At the same time, the influence of paramagnetic subst...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N24/08G01N33/577
Inventor 张锦胜唐群赖卫华
Owner 苏州辅测技术服务有限公司
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