Primer, detection method and detection reagent kit for detecting bacillus coli O157:H7

Abstract

The invention relates a technique for fast detecting food-borne pathogens based on a loop-mediated isothermal amplification, LAMP technique. A primer for diction of colon bacillus O157:H7can augment the specific base sequence of a target gene which is the rfbE-GenBank (accession no. AE005429) of the colon bacillus O157:H7, and the primer is complementary to a part of or a complementary chain fragment of the nucleic acid sequence on the 7462-7471 loci on the target gene. The invention provides a primer having specificity to a specific gene fragment of the colon bacillus O157:H7, and through detecting whether or not the detecting specimen in a reagent box of the primer unit contains the specific gene fragment of the colon bacillus O157:H7, determines whether the colon bacillus O157:H7 exists in the specimen or not.

Description

technical field

[0001] The invention relates to a rapid detection technology for food-borne pathogens based on a loop-mediated isothermal amplification (LAMP) technology. In particular, it relates to a primer and a primer set specific to a specific gene fragment of Escherichia coli O157:H7; it also relates to a detection method and a detection kit for detecting Escherichia coli O157:H7 by a loop-mediated isothermal amplification method using the primers and the primer set . Background technique

[0002] High incidence of foodborne illness, caused by Salmonella, Shigella, Staphylococcus aureus, Proteus, Vibrio cholerae, Vibrio parahaemolyticus and Escherichia coli O157:H7, rotavirus and norovirus Food poisoning, whose incidence rate accounts for a very high proportion in the incidence of foodborne diseases in my country, is a serious public health problem.

[0003] At present, the detection of foodborne pathogens mainly relies on pathogen isolation methods, immunological met...

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