Preservative composition and use thereof

A technology of preservatives and compositions, applied in the field of food additives, can solve the problems of affecting food preservatives and quality, failing to achieve antiseptic effects, and not having inhibition, so as to inhibit the biofilm of food-borne pathogenic bacteria and inhibit food Pathogen-derived biofilm formation, low-cost effects

Active Publication Date: 2016-02-24
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing preservatives only play a role in inhibiting the reproduction of microorganisms in food, and do not have the effect of inhibiting the biofilm activity of food-borne pathogenic bacteria, and preservatives must be added in strict accordance with China's "Hygienic Standards for the Use of Food Additives" , can not exceed the use of
There are many problems in the practical application of preservatives, such as: not reaching the antiseptic effect, affecting the flavor and quality of food, etc.; for example, when tea polyphenols are used as preservatives, the concentration is too high to make people feel bitter and Discoloration of food due to oxidation
However, there is no report about the ability of food additives to inhibit the biofilm formation of food-borne pathogenic bacteria, especially the biofilm formation of Bacillus cereus with strong resistance

Method used

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  • Preservative composition and use thereof
  • Preservative composition and use thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0050] 2.1 Preparation of bacterial suspension

[0051] After activation of Bacillus cereus, it is inoculated into a sterile broth medium and cultured at 37°C for 12 hours until the number of viable bacteria reaches 10 8 CFU / mL, spare.

[0052] 2.2 Preparation of biofilm

[0053] (1) Cleaning of the carrier sheet: Rinse the carrier sheet with natural water and place it in acetone ultrasonic for 15 minutes (ultrasonic frequency 40kHz, ultrasonic power 250W) to remove the surface oil; then, soak it in 75% ethanol for 30 minutes, then Wash with detergent, finally rinse with distilled water, dry and sterilize for later use.

[0054] (2) Cultivation of biofilm: Add carrier sheet and 10mL broth medium to the test tube, after sterilization, insert 1% of the above bacterial suspension (10 8 CFU / mL), incubate at 37°C.

[0055] 2.3 Determination of the number of viable bacteria in the biofilm

[0056] The number of biofilm bacteria is calculated according to the following formula:

[0057]

[0058...

Embodiment 1

[0060] Weigh 0.05g ethyl p-hydroxybenzoate and 0.05g disodium ethylenediaminetetraacetate, add them to 1000mL ethanol solution with a concentration of 30%. After fully dissolving, the preservative with a concentration of 0.1g / L is obtained. combination.

Embodiment 2

[0062] Weigh out 0.03g of ethyl p-hydroxybenzoate and 0.02g of disodium ethylenediaminetetraacetic acid, and add them to 1000mL of 30% ethanol solution. After fully dissolved, the preservative with a concentration of 0.05g / L is obtained. combination.

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Abstract

The present invention relates to the technical field of food additives and particularly relates to a preservative composition and a use thereof. Every 1000 ml of solvent contains 0.005-0.1 g ethyl p-hydroxybenzoate and 0.005-0.1 g of ethylene diamine tetraacetic acid and is prepared by mixing the two materials. The preservative composition contains the ethyl p-hydroxybenzoate and the ethylene diamine tetraacetic acid at a mass ratio of 0.5-1 : 0.5-1. Compared with the prior art, the preservative composition can produce a strong inhibitory effect for the foodborne pathogen biofilm. The present invention provides a new use of the preservative composition in inhibiting the formation of foodborne pathogen biofilm. The preservative composition can be used for the preparations of drugs, food additives and feed additives inhibiting foodborne pathogen biofilm formation,, provides a new path for the control and clearance of biofilm in the food industry, and thereby provides a reliable protection for food safety.

Description

technical field [0001] The invention relates to the technical field of food additives, in particular to a preservative composition and its application. Background technique [0002] With the development of society and the improvement of people's living standards, people pay more and more attention to food safety. However, whether in developing or developed countries, human health is threatened by foodborne diseases. According to statistics, about one-third of the world's people suffer from food-borne diseases every year. [0003] Foodborne pathogenic bacteria are the main factors leading to food spoilage and foodborne diseases, and they are also a long-term problem facing food safety. At present, research work at home and abroad mainly focuses on the free pathogenic spoilage microorganisms in food. However, more and more studies have shown that problems such as bacterial biofilm, sublethal cell repair, and virus mutation are important hidden hazards to food safety and are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L3/3526A61K47/14A61K47/18
CPCA23L3/3526A61K47/14A61K47/183
Inventor 李南薇刘功良高苏娟刘锐
Owner ZHONGKAI UNIV OF AGRI & ENG
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