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Capillary bioanalysis system, and analytical method and applications thereof

A biological analysis and capillary technology, applied in the field of capillary biological analysis system, can solve the problems of simple structure, difficult integration analysis system, single analysis function of capillary analysis system, etc.

Active Publication Date: 2013-11-27
广州市第一人民医院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The capillary analysis system also has some shortcomings, the most prominent of which is that it is difficult to realize complex analysis functions due to its simple structure.
Therefore, capillary analysis systems are often limited to a single analysis function, and it is difficult to appear in the form of an integrated analysis system

Method used

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  • Capillary bioanalysis system, and analytical method and applications thereof
  • Capillary bioanalysis system, and analytical method and applications thereof
  • Capillary bioanalysis system, and analytical method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1 Capillary bioassay system for loop-mediated isothermal amplification.

[0065] Such as image 3 As shown, a capillary bioanalysis system for loop-mediated isothermal amplification includes a three-dimensional motion sampling platform, a temperature control-laser-induced fluorescence detection module, a magnetic field control module, a fluid control unit and a capillary array. The three-dimensional motion sampling platform and the fluid control unit are respectively located at both ends of the capillary array, and the three-dimensional motion sampling platform, temperature control-laser-induced fluorescence detection module, magnetic field control module, and fluid control unit are connected in sequence.

[0066] The three-dimensional motion sampling platform is composed of a reagent-sample tray drive module 1, a reagent-sample tray 2, a lead screw and a capillary array drive module 3; the reagent-sample tray 2 is arranged on top of the reagent-sample tray driv...

Embodiment 2

[0079] Example 2 The loop-mediated isothermal amplification analysis of Mycobacterium tuberculosis was performed using the capillary bioanalysis system for loop-mediated isothermal amplification of Example 1.

[0080] The capillary fitting groove used in the system is provided with 12 parallel capillaries, which are connected to a 12-channel micro-injection pump; the material of the capillary is polytetrafluoroethylene; the inner diameter of the capillary is 1000 microns, and the length is 25 cm.

[0081] Such as Figure 4 As shown, the loop-mediated isothermal amplification analysis of Mycobacterium tuberculosis using the capillary bioanalysis system involves the following steps:

[0082] (1) Introduce 6 μL of mineral oil, 2 μL of magnetic bead suspension for nucleic acid extraction, and 30 μL of lysed sputum sample into the polytetrafluoroethylene capillary in sequence; the magnetic beads are dispersed in 5% glycerin aqueous solution with a mass volume percentage of 0.25 g / ...

Embodiment 3

[0089] Example 3 Capillary bioanalysis system for fluorescent quantitative PCR analysis.

[0090] Such as Figure 6 As shown, a capillary bioanalysis system for fluorescent quantitative PCR analysis includes a three-dimensional motion sampling platform, a temperature control module, a magnetic field control module, a temperature control-laser-induced fluorescence detection module, a fluid control unit and a capillary array. The three-dimensional motion sampling platform and the fluid control unit are respectively located at both ends of the capillary array, and the three-dimensional motion sampling platform, temperature control module, magnetic field control module, temperature control-laser-induced fluorescence detection module, and fluid control unit are connected in sequence.

[0091] The three-dimensional motion sampling platform is composed of a reagent-sample tray drive module 1, a reagent-sample tray 2, a lead screw and a capillary array drive module 3; the reagent-samp...

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Abstract

The invention discloses a capillary bioanalysis system, and an analytical method and applications thereof, and belongs to the field of medical equipment and biological detection technologies. The capillary bioanalysis system comprises a three-dimensional movement sample injecting platform, a temperature control-optical detection module, a magnetic field control module, a fluid control unit and a capillary array. The three-dimensional movement sample injecting platform and the fluid control unit are arranged on the two sides of the capillary array, and the capillary array is provided with the temperature control-optical detection module and the magnetic field control module. According to the capillary bioanalysis system, the droplet technology and the magnetic bead technology are combined, and bioanalysis processes such as loop-mediated isothermal amplification, fluorescence quantitative PCR analysis and immunochemiluminometry are integrated in capillaries. Advantages of the capillary bioanalysis system are that: volume is small, analysis speed is fast, detecting flux is large, and automation degree of operation is high. The capillary bioanalysis system is flexible in application, is suitable for analysis of single sample, batches of samples and on-site rapid detection, is capable of reducing acquisition cost and operation cost of equipment significantly, and possesses excellent economic benefits.

Description

technical field [0001] The invention belongs to the technical field of medical equipment and biological detection, and in particular relates to a capillary biological analysis system and its analysis method and application. Background technique [0002] The capillary analysis system has many advantages: (1) The capillary occupies a small area, which is convenient for parallel analysis; (2) Standard capillaries with different materials and series specifications are available, and the price is low; (3) The consumption of reagents and samples is small; (4) Fluid control is simple. Therefore, capillary tubes are widely used in various types of biological analysis, such as: nucleic acid extraction, nucleic acid amplification, electrophoretic separation, and flow cytometry analysis. [0003] The capillary analysis system also has some deficiencies, the most prominent of which is that it is difficult to realize complex analysis functions due to its simple structure. Therefore, ca...

Claims

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Application Information

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IPC IPC(8): C12M1/42C12M1/38C12Q1/68G01N33/53G01N21/64
Inventor 刘大渔
Owner 广州市第一人民医院
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