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Test method of content of pinoresinol diglucoside in eucommia ulmoides

A technology of pinoresinol diglucoside and measurement method, which is applied in the field of chemical detection, can solve problems such as inconvenient operation, and achieve accurate measurement results

Inactive Publication Date: 2015-11-11
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Existing methods for detecting the content of pinoresinol diglucoside in Eucommia mostly use Soxhlet extractor to heat and reflux, the extraction time is as long as 12 hours, and the operation is very inconvenient

Method used

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  • Test method of content of pinoresinol diglucoside in eucommia ulmoides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] extraction step

[0025] The equipment is ASE350 fast solvent extractor from DIONEX Company of the United States.

[0026] Step 1: Take a sample of Eucommia ulmoides, cut it into pieces, crush it, pass through a No. 3 sieve, take about 1g, weigh it accurately, add diatomaceous earth in a ratio of 1:1 by weight, mix well, and put it in a 10ml extraction pool (sample loading Add an appropriate amount of diatomaceous earth to the bottom of the extraction tank before loading, and fill the extraction tank with diatomaceous earth after loading the sample);

[0027] Step 2: First use n-hexane for static extraction, discard the n-hexane extract, continue to use methanol for static extraction, recover the methanol extract into a 50ml measuring bottle, add methanol to the mark, shake well, filter, and take the subsequent filtrate, The methanol extract is obtained;

[0028] Among them, the parameters for static extraction of n-hexane are extraction temperature 100°C, static extr...

Embodiment 2

[0039] Examination of extraction temperature

[0040] Weigh 12 samples of Eucommia ulmoides from the same batch (batch number: 140601), each about 1g, accurately weighed, and put them in the extraction pool. The static extraction time is 5 minutes, the washing volume is 100%, and the number of cycles is 2 times. Under the conditions of 80°C, 100°C, 120°C, and 140°C, four program extractions were respectively set, the extract was fixed to a 50ml volumetric flask, and samples were injected according to the chromatographic conditions described in step 3 in Example 1, and the results were calculated. See Table 1.

[0041] Table 1 Extraction temperature investigation

[0042]

[0043] It can be seen from Table 1 that the extraction efficiency is basically the same when the extraction temperature is above 100 °C. Although the viscosity of the solvent decreases with the increase of the extraction temperature, thereby enhancing the ability to dissolve the target analyte, but at th...

Embodiment 3

[0045] Investigation of static extraction time

[0046] Weigh 12 samples of Eucommia ulmoides from the same batch (batch number: 140601), each about 1g, accurately weighed, and put them in the extraction pool under the conditions that the extraction temperature is 100°C, the washing volume is 100%, and the number of cycles is 2 times. Next, set static extraction time 3min, 5min, 7min, 9min four program extractions respectively, the extract solution is settled in the 50ml measuring bottle, according to the step 3 chromatographic conditions in the embodiment 1, sample injection, calculation, the results are shown in Table 2.

[0047] Table 2 static extraction time investigation

[0048]

[0049] It can be seen from Table 2 that the extraction time is basically complete when the extraction time is 5 min. Considering saving time and solvent, the extraction time is selected as 5 min.

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Abstract

The invention discloses a test method of content of pinoresinol diglucoside in eucommia ulmoides, wherein the test method comprises the steps of 1) extracting a crushed eucommia ulmoides sample through an ASE extraction method through n-hexane and then methanol, and collecting the methanol extract liquid; and 2) detecting the content of the pinoresinol diglucoside in the eucommia ulmoides through HPLC. The invention aims to provide the test method of the pinoresinol diglucoside in the eucommia ulmoides, which is rapid in extraction, is high in precision and is less in standard deviation.

Description

technical field [0001] The invention relates to the technical field of chemical detection, in particular to a method for measuring the content of pinoresinol diglucoside in Eucommia ulmoides. Background technique [0002] Existing methods for detecting the content of pinoresinol diglucoside in Eucommia mostly use a Soxhlet extractor to heat and reflux. The extraction time is as long as 12 hours, and the operation is very inconvenient. Contents of the invention [0003] The object of the present invention is to provide a method for measuring the content of pinoresinol diglucoside in Eucommia ulmoides with rapid extraction, high precision and small standard deviation. [0004] The technical solution provided by the invention is: a method for measuring the content of pinoresinol diglucoside in Eucommia ulmoides, comprising the following steps: [0005] Step 1: extracting the pulverized Eucommia ulmoides sample by using the ASE extraction method, wherein the ASE extraction me...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/14
Inventor 陈学松王丽丽
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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