Preparation method for standardized extracts of eucommia ulmoides pinoresinol diglucoside
A technology of pinoresinol diglucoside and extract, which is applied in the field of extracting active ingredients of plants, can solve problems such as unstable quality and efficacy, and difficulty in controlling the content of active ingredients, so as to achieve reduced usage, lower production costs, and high extraction efficiency Effect
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Embodiment 1
[0022] Take 1000g of Eucommia bark, crush it, pass through a 20-mesh sieve, put it into a 20L ultrasonic-assisted extraction tank, add 15L of purified water, stir, heat it to 50°C, extract for 60min, and centrifugally filter; the centrifugate passes through a 0.1μm microporous filter, fine filtrate Ultrafiltration through a 50,000Da polysulfone roll-type membrane, the ultrafiltration retentate is reused as extraction process water, and the ultrafiltration dialysate is adsorbed on a macroporous resin; the adsorption column is 30×400mm, the resin model is D101, and the saturated adsorption capacity of PDG is 56.0mg / g. The volume on the column is 10.5BV (column volume, the same below), the flow rate is 2BV / h, until the point of PDG leakage occurs (the PDG content in the effluent is ≥0.1mg / ml, the same below), and the concentration is 30% and 50% Ethanol-water solution was used for gradient elution, the eluate was collected in sections, thin-layer spotting was detected, the same co...
Embodiment 2
[0024] Take 1000g of eucommia bark, crush it, pass through a 40-mesh sieve, put it into a 20L microwave-assisted extraction tank, add 12L of purified water, add an appropriate amount of sulfuric acid, and adjust the pH value to 4; add 84g of cellulase, heat to 50°C, and enzymatically hydrolyze for 2 hours. Centrifugal filtration; the centrifugate is passed through a 0.1μm microporous filter, the fine filtrate is passed through a 50,000Da polysulfone roll-type membrane ultrafiltration, the ultrafiltration retentate is reused as extraction process water, and the ultrafiltration dialysate is adsorbed on a macroporous resin; the adsorption column is 30×400mm , Resin model AB-8, PDG saturated adsorption capacity 62.29mg / g, loading volume 10.5BV, flow rate 2BV / h, until PDG leakage occurs; elute with 50% ethanol-water solution, collect the eluate in sections , thin-layer spotting detection, combining the same components; upper C 18 Reverse-phase silica gel column chromatography, flow...
Embodiment 3
[0026] Take 1000g of Eucommia bark, crush it, pass through a 60-mesh sieve, put it into a 20L microwave-assisted extraction tank, add 10L of purified water, heat it to 40°C, extract for 45 minutes, and centrifugally filter; 50000Da polysulfone roll-type membrane ultrafiltration, the ultrafiltration retentate is reused as extraction process water, and the ultrafiltration dialysate is adsorbed on a macroporous resin; the adsorption column is 30×400mm, the resin model is S-8, and the saturated adsorption capacity of PDG is 63.89mg / g , the amount on the column is 8.5BV, the flow rate is 2BV / h, until the point of PDG leakage; elute with 50% ethanol-water solution, collect the eluate in sections, detect by thin layer spotting, and combine the same components; above C 18 Reverse-phase silica gel column chromatography, flow rate 2BV / h, until the PDG breakthrough point is saturated and adsorbed; elute with 30% methanol-water solution, flow rate 0.5-1.0BV / h, collect eluate in sections, a...
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